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41.
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Degradation of unpretreated and thermally pretreated polypropylene by soil consortia 总被引:1,自引:0,他引:1
Ambika Arkatkar J. Arutchelvi Sumit Bhaduri Parasu Veera Uppara Mukesh Doble 《International biodeterioration & biodegradation》2009,63(1):106-111
Unpretreated (PP-UT) and thermally pretreated (at 80 °C for 10 days) polypropylene (PP-TT) films of 0.05 mm thickness were subjected to in vitro biodegradation in minimal medium with mixed soil culture for 12 months. In this period 10.7 and 0.4% weight loss was observed with PP-TT and PP-UT, respectively. The tensile strength decreased by 51.8 and 28.3%, the crystallinity increased by 28 and 33% and isotacticity increased by 3 and 9%, respectively, over the same time period. The ester carbonyl index in PP-TT increased up to 9 months and later decreased indicating abiotic followed by biotic process. No such changes were observed with PP-UT. Methyl group index decreased in both the cases indicating oxidation at the primary carbon. Increase in surface energy indicated that the polymer became hydrophilic. Surface changes were observed by SEM and AFM. A single culture was isolated at the end of 12 months and it was identified as Bacillus flexus. The morphology of the organism was rods in a chain and it was present in the form of an endospore. 相似文献
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Kainulainen V Loimaranta V Pekkala A Edelman S Antikainen J Kylväjä R Laaksonen M Laakkonen L Finne J Korhonen TK 《Journal of bacteriology》2012,194(10):2509-2519
Glutamine synthetase (GS) and glucose-6-phosphate isomerase (GPI) were identified as novel adhesive moonlighting proteins of Lactobacillus crispatus ST1. Both proteins were bound onto the bacterial surface at acidic pHs, whereas a suspension of the cells to pH 8 caused their release into the buffer, a pattern previously observed with surface-bound enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of L. crispatus. The pH shift was associated with a rapid and transient increase in cell wall permeability, as measured by cell staining with propidium iodide. A gradual increase in the release of the four moonlighting proteins was also observed after the treatment of L. crispatus ST1 cells with increasing concentrations of the antimicrobial cationic peptide LL-37, which kills bacteria by disturbing membrane integrity and was here observed to increase the cell wall permeability of L. crispatus ST1. At pH 4, the fusion proteins His(6)-GS, His(6)-GPI, His(6)-enolase, and His(6)-GAPDH showed localized binding to cell division septa and poles of L. crispatus ST1 cells, whereas no binding to Lactobacillus rhamnosus GG was detected. Strain ST1 showed a pH-dependent adherence to the basement membrane preparation Matrigel. Purified His(6)-GS and His(6)-GPI proteins bound to type I collagen, and His(6)-GS also bound to laminin, and their level of binding was higher at pH 5.5 than at pH 6.5. His(6)-GS also expressed a plasminogen receptor function. The results show the strain-dependent surface association of moonlighting proteins in lactobacilli and that these proteins are released from the L. crispatus surface after cell trauma, under conditions of alkaline stress, or in the presence of the antimicrobial peptide LL-37 produced by human cells. 相似文献
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David Wheeler Edward Knapp Veera V. R. Bandaru Yue Wang† David Knorr‡ Christophe Poirier§ Mark P. Mattson†¶ Jonathan D. Geiger‡ Norman J. Haughey 《Journal of neurochemistry》2009,109(5):1237-1249
The insertion and removal of NMDA receptors from the synapse are critical events that modulate synaptic plasticity. While a great deal of progress has been made on understanding the mechanisms that modulate trafficking of NMDA receptors, we do not currently understand the molecular events required for the fusion of receptor containing vesicles with the plasma membrane. Here, we show that sphingomyelin phosphodiesterase 3 (also known as neutral sphingomyelinase-2) is critical for tumor necrosis factor (TNF) α-induced trafficking of NMDA receptors and synaptic plasticity. TNFα initiated a rapid increase in ceramide that was associated with increased surface localization of NMDA receptor NR1 subunits and a specific clustering of NR1 phosphorylated on serines 896 and 897 into lipid rafts. Brief applications of TNFα increased the rate and amplitude of NMDA-evoked calcium bursts and enhanced excitatory post-synaptic currents. Pharmacological inhibition or genetic mutation of neutral sphingomyelinase-2 prevented TNFα-induced generation of ceramide, phosphorylation of NR1 subunits, clustering of NR1, enhancement of NMDA-evoked calcium flux and excitatory post-synaptic currents. 相似文献
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Manickaraj AshokKumar Navaneethan Gnana Veera Subhashini Ramineni SaiBabu Arabandi Ramesh Kotturathu Mammen Cherian Cyril Emmanuel 《Molecular biology reports》2010,37(1):521-527
Apolipoprotein C3 and apolipoprotien A5 are proteins coded from the APOA1/C3/A4/A5 gene cluster. Sst I polymorphism on apolipoprotein
C3 and −1131C polymorphism of apolipoprotien A5 are key variants involved in triglyceride metabolism and cause a significant
cardio-metabolic risk. Here, we have evaluated these two variants for their roles in coronary artery disease in patients of
the Indian population. The apolipoprotein gene cluster variants were analysed in 416 angiographically determined coronary
artery disease patients and matched 416 controls using polymerase chain reaction—restriction fragment length polymorphism.
The characteristics of the study subjects were analyzed statistically for their association with the polymorphisms. The alleles
were combined as haplotypes and their combined risks were evaluated. The minor allele genotypes of both apolipoprotein C3
(S2) and apolipoprotien A5 (C) had a significant risk for coronary artery disease. The S2 allele genotyped patients had a
significantly increased triglyceride level (P < 0.001) and increased triglycerides were observed among both patient and control CC genotype carriers. We identified the
haplotype S2/C with a significant increased risk (P < 0.001) to coronary artery disease with increased levels of circulating triglycerides compared to other haplotypes in patients.
We conclude that the variants on apolipoprotein C3 and apolipoprotien A5 modulate serum triglyceride levels and increase the
risk of coronary artery disease. 相似文献
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The physicochemical characteristics of the green fluorescent protein (GFP), including the thermodynamic properties (entropy, enthalpy, Gibbs' free energy, heat capacity), normal mode vibrations, and atomic fluctuations, were investigated. The Gaussian 03 computational chemistry program was employed for normal mode analysis using the AMBER force field. The thermodynamic parameters and atomic fluctuations were then calculated from the vibrational eigenvalues (frequencies) and eigenvectors. The regions of highest rigidity were shown to be the beta-sheet barrel with the central alpha-helix, which bears the chromophore. The most flexible parts of the GFP molecule were the outlying loops that cover the top and bottom of the beta-barrel. This way, the balance between rigidity and flexibility is maintained, which is the optimal relationship for protein stability in terms of Gibbs' free energy. This dual-schemed structure satisfies the requirements for GFP function. In this sense, the structure of GFP resembles a nanoscale drum: a stiff cylinder with flexible vibrating end(s). 相似文献