Isolation,culture, and identification of ceruminous gland cells

Journal of Molecular Histology - External auditory canal (EAC) stenosis or atresia usually requires a skin graft to repair, but due to the lack of a graft containing functional glands,...     

中国隐胫瓢虫亚科Aspidimerinae的新种及新记录：（鞘翅目：瓢虫科）

本文记述了中国隐胫瓢虫亚科Ａｓｐｉｄｉｍｅｒｉｎａｅ３属３４种，其中包括１亲记录属：拟隐胫瓢虫属Ｐｓｅｕｄａｓｐｉｄｉｍｅｒｕｓ；１新记录种：棕环拟隐胫瓢虫Ｐｓｅｕｄａｓｐｉｄｉｍｅｒｕｓ ｌｏｍｂａｔｕｓ Ｈｏａｎｇ，１９８３和３新种：舌端隐势瓢虫Ｃｒｙｐｔｏｇｏｎｕｓ ｌｉｎｇｕｉｌａｔｕｓ，曲斑隐势瓢虫Ｃｒｙｐｔｏｇｏｎｕｓ ｆｒａｃｔｅｍａｃｕｌａｔｕｓ及短叶隐势瓢虫Ｃｒｙｐｔｏｇｏｎｕｓ     

The role of extracellular vesicles in podocyte autophagy in kidney disease

Podocytes are the key cells involved in protein filtration in the glomerulus. Once proteins appear in the urine when podocytes fail, patients will end with renal failure due to the progression of glomerular damage if no proper treatment is applied. The injury and loss of podocytes can be attributed to diverse factors, such as genetic, immunologic, toxic, or metabolic disorders. Recently, autophagy has emerged as a key mechanism to eliminate the unwanted cytoplasmic materials and to prolong the lifespan of podocytes by alleviating cell damage and stress. Typically, the fundamental function of extracellular vesicles (EVs) is to mediate the intercellular communication. Recent studies have suggested that, EVs, especially exosomes, play a certain role in information transfer by communicating proteins, mRNAs, and microRNAs with recipient cells. Under physiological and pathological conditions, EVs assist in the bioinformation interchange between kidneys and other organs. It is suggested that EVs are related to the pathogenesis of acute kidney injury and chronic kidney disease, including glomerular disease, diabetic nephropathy, renal fibrosis and end-stage renal disease. However, the role of EVs in podocyte autophagy remains unclear so far. Here, this study integrated the existing information about the relevancy, diagnostic value and therapeutic potential of EVs in a variety of podocytes-related diseases. The accumulating evidence highlighted that autophagy played a critical role in the homeostasis of podocytes in glomerular disease.     

Hydrogen sulfide and metal interaction: the pathophysiological implications

Hydrogen sulfide (H₂S), previously recognized as a toxic gas, has emerged as an important gaseous signaling molecule along with nitric oxide, carbon monoxide and also hydrogen. H₂S can be endogenously produced in the mammalian body at a very low level for various pathophysiological processes. Notably, H₂S can interact with several essential metals in the body such as iron, copper, nickel, and zinc to carry out specific functions. The interactions of H₂S with metal-binding proteins have been shown to aid in its signal transduction and cellular metabolism. In addition, H₂S is capable of providing a cytoprotective role against metal toxicity. As the research in the field of H₂S signaling in biology and medicine increases, much progresses have been developed for detecting H₂S via interaction with metals. In this review, the interaction of H₂S with metals, specifically in regard to metal-driven metabolism of H₂S, the protection against metal toxicity by H₂S and the detection of H₂S using metals will be discussed. Discovering the interactions of this gasotransmitter with metals is important for determining the mechanisms underlying the cellular functions of H₂S as well as developing novel therapeutic avenues.

     

Detection of infectious dengue virus by selective real-time quantitative polymerase chain reaction

Dear Editor,The dengue virus(DENV)is a single-stranded positivesense RNA virus that belongs to the family Flaviviridae(Gubler,2002),and has four serotypes,DENV1–DENV4,which are transmitted via Aedes aegypti and Aedes albopictus(Rodriguez-Roche and Gould,2013).It has been reported that more than 50 million dengue infections occur each year(Guzman et al.,2010),and a serious outbreak occurred in the Southern Provinces of     

H2S is involved in ABA-mediated stomatal movement through MPK4 to alleviate drought stress in Arabidopsis thaliana

Plant and Soil - Hydrogen sulfide (H2S) is a gaseous signaling molecule that participates in multiple physiological processes in both animals and plants. Mitogen-activated protein kinase (MAPK) is...