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861.
Saedi TA Moeini H Tan WS Yusoff K Daud HM Chu KB Tan SG Bhassu S 《Molecular biology reports》2012,39(5):5785-5790
White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M. rosenbergii cultured in Malaysia. In the present study, RT-PCR assay was used to detect the causative agents of WTD, M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples. Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from
the other isolates. Histopathological studies revealed myofiber degeneration of the tail muscles and liquefactive myopathy
in the infected prawns. This was the first report on the occurrence of MrNV in the Malaysian freshwater prawn. 相似文献
862.
High-grade gliomas are difficult to treat. We examined the therapeutic effect of intratumoral administration of human amniotic
membrane derived-mesenchymal stem cells (hAMCs) on the growth of gliomas. Tumor volume of the control group was 1632 ± 316 mm3 on day 30, and the group treated with a single intratumoral dose of hAMCs had a tumor volume of 1128 ± 269 mm3 (P < 0.05). Thus, administration of hAMCs significantly reduced tumor size. In rat glioma tissues treated with single and multiple
dosages of hAMCs, there was a reduction in tumor volume of approximately 30.9 and 49.5%, respectively. We further evaluated
the glioma tissue using Western blotting analysis and observed that the expression of Bax, caspase-8 and caspase-3 were greatly
increased and the expression of Bcl-2 was greatly decreased in tumors treated with hAMCs. Sections of nude mice treated with
hAMCs clearly showed the presence of an increase in apoptotic cells. The data collected herein confirms for the first time
that hAMCs can inhibit C6 glioma growth and induce apoptosis of C6 gliomas in vivo. This demonstrates that hAMCs are a potential
new therapeutic agent for the treatment of gliomas. 相似文献
863.
Bao Zhang Yi Kun Guo Shengbin Li Liang Zhi Zhang Xian Yong Lan Shen Rong Hu Hong Chen 《Molecular biology reports》2012,39(12):10475-10479
Zinc-a2-glycoprotien (AZGP1) involved in lipid metabolism and associated with adipose tissue atrophy in cachexia. And it also related to sperm motility and in turn fertilization. To ascertain whether there were mutations in the bovine AZGP1 gene, this study investigated variation of the AZGP1 gene through PCR-SSCP and sequencing. Four missense mutations were identified in 649 cattle from six independent populations. Haplotype frequencies and linkage disequilibrium (LD) coefficients of these SNPs in three Chinese indigenous cattle breeds were analyzed. One LD block was found in three cattle breeds. The statistical analyses indicated that AC genotype of Z4 locus was associated with the high body weight, body length and chest girth in Jiaxian cattle breed (P?<?0.05). Our results provided evidence that polymorphisms in the AZGP1 gene were associated with growth traits, and may be used for marker-assisted selection and management in cattle breeding program. 相似文献
864.
865.
Gap junction provides intercellular communications that play a critical role in invasion of metastatic cancer cells. However,
the effects of inhibiting this pathway in breast cancer cell migration have not been investigated. Here, we present data demonstrating
that functional blockade of gap junctions during the formation of monolayer decreased the levels of aligned fibers of actin
between neighboring breast cancer cells. Furthermore, gap junction inhibitors attenuated the invasion ability of highly metastatic
MDA-MB-231 cells, but had no significant effects on less invasive MCF-7 cells, which caused by shRANKL. Our work is the first
to demonstrate the inhibitory effect of gap junction channel inhibitors on the migration of highly invasive breast cancer
cells. 相似文献
866.
Li JM Yao ZF Zou YZ Ge JB Guan AL Wu J Mi SL Liang YY Ma Z 《Molecular biology reports》2012,39(1):5-12
In animal models of clinical entities causative of severe right and left ventricular (LV) pressure overload hypertrophy, increased
density of the cellular microtubule network, through viscous loading of active myofilaments, causes contractile dysfunction
that is normalized by microtubule depolymerization. In this study, 86 male mice were divided into seven groups. The transverse
ascending aorta constriction (TAC) in six groups were performed in order to make heart failure model. Mice in each group were
injected with G-CSF or/and telmisartan subcutaneously at different time respectively. Results showed that reduction in left
ventricular volume and improved function persisted at 2 week, but recurrent dilatation at 4 weeks was associated with a loss
of functional improvement. Compared with PBS group, the expression of VEGF protein and HIF-1 mRNA were significantly higher
in mice injected with G-CSF or/and telmisartan (P < 0.05). The expression of p53 mRNA, myocardial fibrosis and mortality were significantly lower in mice injected with G-CSF
or/and telmisartan (P < 0.05). It could be concluded that G-CSF can delay the progression of pressure overload induced ventricular reconstruction
and heart failure in mice. 相似文献
867.
Tsai MS Yang YL Wang AH Wang LS Lu DC Liou CH Hsieh LY Wu CJ Cheng MF Shi ZY Lo HJ 《Mycopathologia》2012,174(2):121-130
A total of 35 Trichosporon isolates were collected from the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) project from 1999 to 2006, and their identifications as well as drug susceptibilities were determined. The most frequently isolated species was T. asahii (62.9%), and the most common clinical sample that yielded Trichosporon isolates was urine (37.1%). The etiology of all seven invasive trichosporonosis was T. asahii. For the 22 T. asahii isolates, the MIC(50) and MIC(90) for amphotericin B were 0.25 and 1 μg/mL, respectively. Those for fluconazole were 2 and 4 μg/mL, respectively, and for voriconazole 0.031 and 0.063 μg/mL, respectively. When the intraclass correlation coefficients (ICCs) and agreements were calculated, we found that the MICs of fluconazole obtained from different methods were similar and the inter-method discrepancies were low. Nevertheless, no unanimous MIC of amphotericin B and voriconazole was obtained among different methods. 相似文献
868.
869.
870.
The pH-modified citrus pectin (MCP) has been demonstrated to inhibit galectin-3 in cancer progression. The components and structures of MCP related to this inhibition remained unknown. In this paper, we fractionated MCP on DEAE-cellulose column into a homogenous neutral fraction MCP-N (about 20?kDa) and a pectin mixture fraction MCP-A (wide molecular distribution on Sepharose CL-6B chromatography). Both MCP-N and MCP-A inhibited hemagglutination mediated by galectin-3 with minimum inhibition concentration (MIC) 625 and 0.5?μg/ml, respectively. MCP-N was identified to be a type I arabinogalactan (AG-I) with a main chain of β-1→4-galactan. MCP-N was digested by α-L-arabinofuranosidase to give its main chain structure fraction (M-galactan, around 18?kDa), which was more active than the original molecule, MIC 50?μg/ml. The acidic degradation of M-galactan increased the inhibitory activity, MIC about 5 times lower than M-galactan. These results above showed that the functional motif of the β-1→4-galactan fragment might lie in the terminal residues rather than in the internal region of the chain. Therefore, MCP-N and its degraded products might be developed to new potential galectin-3 inhibitors. This is the first report concerning the fractionation of MCP and its components on galectin-3 inhibition. The information provided in this paper is valuable for screening more active galectin-3 inhibitors from natural polysaccharides. 相似文献