全文获取类型
收费全文 | 94篇 |
免费 | 19篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2012年 | 9篇 |
2011年 | 6篇 |
2010年 | 1篇 |
2009年 | 5篇 |
2008年 | 4篇 |
2007年 | 2篇 |
2006年 | 7篇 |
2005年 | 2篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有113条查询结果,搜索用时 31 毫秒
11.
Bandyopadhyay S Gama F Molina-Navarro MM Gualberto JM Claxton R Naik SG Huynh BH Herrero E Jacquot JP Johnson MK Rouhier N 《The EMBO journal》2008,27(7):1122-1133
Glutaredoxins (Grxs) are small oxidoreductases that reduce disulphide bonds or protein-glutathione mixed disulphides. More than 30 distinct grx genes are expressed in higher plants, but little is currently known concerning their functional diversity. This study presents biochemical and spectroscopic evidence for incorporation of a [2Fe-2S] cluster in two heterologously expressed chloroplastic Grxs, GrxS14 and GrxS16, and in vitro cysteine desulphurase-mediated assembly of an identical [2Fe-2S] cluster in apo-GrxS14. These Grxs possess the same monothiol CGFS active site as yeast Grx5 and both were able to complement a yeast grx5 mutant defective in Fe-S cluster assembly. In vitro kinetic studies monitored by CD spectroscopy indicate that [2Fe-2S] clusters on GrxS14 are rapidly and quantitatively transferred to apo chloroplast ferredoxin. These data demonstrate that chloroplast CGFS Grxs have the potential to function as scaffold proteins for the assembly of [2Fe-2S] clusters that can be transferred intact to physiologically relevant acceptor proteins. Alternatively, they may function in the storage and/or delivery of preformed Fe-S clusters or in the regulation of the chloroplastic Fe-S cluster assembly machinery. 相似文献
12.
V. Morawala-Patell J. M. Gualberto L. Lamattina J.-M. Grienenberger G. Bonnard 《Molecular & general genetics : MGG》1998,258(5):503-511
In wheat mitochondria, the gene coding for subunit 2 of the NADH-ubiquinone oxidoreductase (nad2) is divided into five exons located in two distant genomic regions. The first two exons of the gene, a and b, lie 22 kb downstream
of exons c, d, and e, on the same DNA strand. All introns of nad2 are group II introns. A trans-splicing event is required to join exons b and c. It involves base pairing of the two precursor
RNAs in the stem of domain IV of the intron. A gene coding for tRNATyr is located upstream of exon c. In addition to splicing processes, mRNA editing is also required for the correct expression
of nad2. The mature mRNA is edited at 36 positions, distributed over its five exons, resulting in 28 codon modifications. Editing
increases protein hydrophobicity and conservation.
Received: 11 August 1997 / Accepted: 2 February 1998 相似文献
13.
Gualberto Pacheco‐Sierra Zachariah Gompert Jerónimo Domínguez‐Laso Ella Vázquez‐Domínguez 《Molecular ecology》2016,25(14):3484-3498
Hybrid zones represent natural laboratories to study gene flow, divergence and the nature of species boundaries between closely related taxa. We evaluated the level and extent of hybridization between Crocodylus moreletii and Crocodylus acutus using genetic and morphological data on 300 crocodiles from 65 localities. To our knowledge, this is the first genetic study that includes the entire historic range and sympatric zone of the two species. Contrary to expectations, Bayesian admixture proportions and maximum‐likelihood estimates of hybrid indexes revealed that most sampled crocodiles were admixed and that the hybrid zone is geographically extensive, extending well beyond their historical region of sympatry. We identified a few geographically isolated, nonadmixed populations of both parental species. Hybrids do not appear to be F1s or recent backcrosses, but rather are more likely later‐generation hybrids, suggesting that hybridization has been going on for several to many generations and is mostly the result of natural processes. Crocodylus moreletii is not the sister species of C. acutus, suggesting that the hybrid zone formed from secondary contact rather than primary divergence. Nonadmixed individuals from the two species were distinguishable based on morphological characters, whereas hybrids had a complex mosaic of morphological characters that hinders identification in the wild. Very few nonadmixed C. acutus and C. moreletii populations exist in the wild. Consequently, the last nonadmixed C. moreletii populations have become critically endangered. Indeed, not only the parental species but also the naturally occurring hybrids should be considered for their potential conservation value. 相似文献
14.
Pablo?Ramon?Gualberto?Cardoso Emerson?Vasconcelos?de?Andrade Lima Mariana?Modesto?de?Andrade Lima Moacyr?Jesus?Barreto?de?Melo Rêgo Claudia?Diniz?Lopes?Marques Ivan?da?Rocha Pitta Angela?Luzia?Branco?Pinto?Duarte Maira?Galdino?da?Rocha PittaEmail author 《European cytokine network》2016,27(1):1-5
Objective and Design
Psoriasis is a common, enigmatic, and recurrent disease. The precise etiology and pathogenesis of psoriasis are still unclear. Psoriasis has been treated as an inflammatory disorder related to an underlying Th1/Th17-dominated immune response. Interleukins are involved in the development of psoriasis lesions through Th-17-associated inflammation. Th1 and Th17 cytokines are found in skin lesions and in the peripheral blood of psoriasis patients.We sought to analyze serum levels of IL-1-β, IL-8, IL-9, IL-27, IL-29, IL-35, IFN-γ, TNF and TGF-β in patients with psoriasis and healthy control volunteers.Material
Blood samples were collected from fifty-three patients with psoriasis and thirty-five healthy controls.Methods
Serum cytokines concentrations were determined using an enzyme-linked immunosorbent assay.Results
Serum IL-8, IL-9, IL-27, IL-29 and TNF levels were statistically significant in psoriasis patients. Detectable serum IL-9 levels were found in 47 patients of the 53 in the psoriasis group.Conclusions
Interleukins-8, 27, 29 and TNF levels measured in the serum of psoriasis patients were slightly elevated as compared to healthy controls in a weakly significant way. On the other hand, there were highly significant differences in IL-9 levels between the two groups.15.
16.
Miguel del Corral JM Castro MA Oliveira AB Gualberto SA Cuevas C San Feliciano A 《Bioorganic & medicinal chemistry》2006,14(21):7231-7240
A series of new furoterpenyl-1,4-naphtho(anthra)quinones have been prepared via oxidative cyclization of the corresponding 2-hydroxy-3-butenyl-1,4-naphtho(anthra)quinones. Depending on the reaction conditions the 1,2-quinones or the 1,4-quinones were obtained. Several new furo-1,4-anthraquinones were also obtained by condensation of 2,3-dichloroquinones with 1,3-dicarbonyls. The compounds synthesized have been evaluated for their cytotoxicity against neoplastic cell lines, some of them being effective below the micromolar level. 相似文献
17.
Johannes A Hofberger Beifei Zhou Haibao Tang Jonathan DG Jones M Eric Schranz 《BMC genomics》2014,15(1)
Background
Recent advances in DNA sequencing techniques resulted in more than forty sequenced plant genomes representing a diverse set of taxa of agricultural, energy, medicinal and ecological importance. However, gene family curation is often only inferred from DNA sequence homology and lacks insights into evolutionary processes contributing to gene family dynamics. In a comparative genomics framework, we integrated multiple lines of evidence provided by gene synteny, sequence homology and protein-based Hidden Markov Modelling to extract homologous super-clusters composed of multi-domain resistance (R)-proteins of the NB-LRR type (for NUCLEOTIDE BINDING/LEUCINE-RICH REPEATS), that are involved in plant innate immunity.Results
To assess the diversity of R-proteins within and between species, we screened twelve eudicot plant genomes including six major crops and found a total of 2,363 NB-LRR genes. Our curated R-proteins set shows a 50% average for tandem duplicates and a 22% fraction of gene copies retained from ancient polyploidy events (ohnologs). We provide evidence for strong positive selection and show significant differences in molecular evolution rates (Ka/Ks-ratio) among tandem- (mean = 1.59), ohnolog (mean = 1.36) and singleton (mean = 1.22) R-gene duplicates. To foster the process of gene-edited plant breeding, we report species-specific presence/absence of all 140 NB-LRR genes present in the model plant Arabidopsis and describe four distinct clusters of NB-LRR “gatekeeper” loci sharing syntenic orthologs across all analyzed genomes.Conclusion
By curating a near-complete set of multi-domain R-protein clusters in an eudicot-wide scale, our analysis offers significant insight into evolutionary dynamics underlying diversification of the plant innate immune system. Furthermore, our methods provide a blueprint for future efforts to identify and more rapidly clone functional NB-LRR genes from any plant species.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-966) contains supplementary material, which is available to authorized users. 相似文献18.
Alison M Clargo Ashley R Hudson Welcome Ndlovu Rebecca J Wootton Louise A Cremin Victoria L O'Dowd Carla R Nowosad Dale O Starkie Sophie P Shaw Joanne E Compson Dominic P White Brendon MacKenzie James R Snowden Laura E Newnham Michael Wright Paul E Stephens Meryn R Griffiths Alastair DG Lawson Daniel J Lightwood 《MABS-AUSTIN》2014,6(1):143-159
19.
A Gualberto D LePage G Pons S L Mader K Park M L Atchison K Walsh 《Molecular and cellular biology》1992,12(9):4209-4214
20.
RNA editing in plant mitochondria and chloroplasts 总被引:17,自引:0,他引:17