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991.
Lettnin Aline Portantiolo Wagner Eduardo Felipe Carrett-Dias Michele dos Santos Machado Karina Werhli Adriano Cañedo Andrés Delgado Trindade Gilma Santos de Souza Votto Ana Paula 《Molecular biology reports》2019,46(2):1873-1884
Molecular Biology Reports - Cancer stem cells show epigenetic plasticity and intrinsic resistance to anti-cancer therapy, rendering capable of initiating cancer relapse and progression.... 相似文献
992.
María de Lourdes Ramírez‐Ahuja Mayra A. Gmez‐Govea Angel Lugo‐Trampe Gissela Borrego‐Soto Ivn Delgado‐Enciso Gustavo Ponce‐Garcia Margarita L. Martínez‐Fierro Eda G. Ramírez‐Valles Víctor Trevio Adriana E. Flores‐Suarez Iram P. Rodríguez‐Sanchez 《Journal of Applied Entomology》2019,143(8):834-841
The microbiota of Hymenoptera is limited to certain bacterial species that vary according to the habitat and diet; however, the bacterial populations are not known in Telenomus tridentatus Johnson & Bin, an egg parasitoid of agricultural importance. We determined the microbiota composition of adults of T. tridentatus using next‐generation sequencing technologies. We found the presence of the Phylums Proteobacteria, in greater proportion followed by Actinobacteria and Firmicutes, respectively. The most abundant species were Cutibacterium acnes, Aquabacterium sp. and Massilia sp. The results of this study could lead to investigating the importance of symbiotic bacteria in T. tridentatus and its relation with agricultural plantations. 相似文献
993.
de la Cruz JO García-Soto J Uriostegui C Carranza L Novoa G Reyna G Martínez-Cadena G 《Canadian journal of microbiology》2007,53(2):168-176
Evidence has been obtained that indicates the presence of small 22 kDa GTP-binding Rho proteins through ADP-ribosylation by Clostridium botulinum C3 exotoxin in Mucor circinelloides. Rho protein was detected at all stages of growth studied. During polarized growth, both under aerobic conditions and during the yeast-mycelia transition, the radiolabeling of the [32P]ADP-ribosylated protein increased when tube formation occurred and decreased as the hyphae branched. However, when Mucor grew isotropically, the Rho protein band was thick and its intensity did not vary significantly even after bud formation and separation of daughter cells. Crude extracts of yeast and mycelial cells exhibited a broad 22 kDa band of the [32P]ADP-ribosylated Rho protein that was resolved into a protein with a pI of 6.0, after two-dimensional electrophoresis, corresponding to the Rho1p homolog. Furthermore, [32P]ADP-ribosylated Rho protein from soluble and particulate extracts of multipolarized mycelial cells obtained from the yeast-mycelia transition was separated into two proteins with pI of 6.0 and 6.4, respectively, after two-dimensional electrophoresis. These correspond to the Rho1p and Rho3p homologs, respectively. Therefore, our results show that an increase in Rho accumulation is associated with polarized growth. 相似文献
994.
995.
To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes. The permeability to water (microm/min/atm) of immature oocytes at 25 degrees C (0.37) was significantly higher than that of mature oocytes (0.10). The permeability (x10(-3)cm/min) of immature oocytes to ethylene glycol, propylene glycol, and Me(2)SO (1.49-3.03) at 25 degrees C was substantially higher than that of mature oocytes approximately 0. The permeability of immature oocytes to glycerol was also high (1.75), although the permeability could not be measured in mature oocytes. Immature oocytes would be more suitable than mature oocytes for conservation of the zebrafish. 相似文献
996.
Arranz N Haza AI García A Delgado E Rafter J Morales P 《Chemico-biological interactions》2007,169(1):63-71
The aim of this study was to evaluate the effects of organosulfurs, isothiocyanates and vitamin C towards hydrogen peroxide-induced DNA damage (DNA strand breaks and oxidized purines/pyrimidines) in human hepatoma cells (HepG2), using the Comet assay. Treatment with hydrogen peroxide (H(2)O(2)) increased the levels of DNA strand breaks and oxidized purine and pyrimidine bases, in a concentration and time dependent manner. Organosulfur compounds (OSCs) reduced DNA strand breaks induced by H(2)O(2). In addition, OSCs also decreased the levels of oxidized pyrimidines. However, none of the OSCs tested reduced the levels of oxidized purines. Isothiocyanates compounds (ITCs) and vitamin C showed protective effects towards H(2)O(2)-induced DNA strand breaks and oxidized purine and pyrimidine bases. The results indicate that removal of oxidized purine and pyrimidine bases by ITCs was more efficient than by OSCs and vitamin C. Our findings suggest that OSCs, ITCs and vitamin C could exert their protective effects towards H(2)O(2)-induced DNA strand breaks and oxidative DNA damage by the free radical-scavenging efficiency of these compounds. 相似文献
997.
Fasciola hepatica: identification of CD4+ T-helper epitopes from the 11.5 kDa saposin-like protein SAP-2 using synthetic peptides 总被引:1,自引:0,他引:1
Espino AM Torres D Morales A Delgado B Quetel J Osuna A 《Experimental parasitology》2007,117(1):65-73
Fasciola hepatica saposin-like protein (FhSAP-2) is a novel antigen expressed at an early stage of infection and has been shown to induce in rabbits a significant protection to infection with F. hepatica. There are no studies to identify the immunologically relevant regions of FhSAP-2. In this work the amino acid sequence of FhSAP-2 was analyzed to identify potential T-cell epitopes. A predictive algorithm identified four possible sites. Experimental determination of the T-cell epitopes was achieved using a panel of overlapping peptides spanning the entire sequence of FhSAP-2, which was evaluated for their ability to induce lymphoproliferative responses of spleen cells from 8 immunized BALB/c (H-2d) mice. Five different epitopes were identified. There was minimal agreement between theoretical and experimental approaches. It was found that peptides containing amino acid residues AVTFA and IDIDLCDICT as part of their structure induce high levels of IL-2 and IFNgammain vitro and was classified as Th1 epitopes. Peptides that contain the residues ADQTV, CIEFVQQEVD and YIIDHVDQHN induced significant amount of IL-4 and IL-2 were considered as containers of Th0 epitopes. Identification of prominent T-cell epitopes from FhSAP-2 offers the possibility of understanding how the CD4+ T-cell response is involved in protection against fasciolosis and how it is implicated in susceptibility to infection. 相似文献
998.
Fernández-Velasco M Ruiz-Hurtado G Hurtado O Moro MA Delgado C 《American journal of physiology. Heart and circulatory physiology》2007,293(1):H238-H245
Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that has been implicated in the pathogenesis of heart failure. Prolongation of the action potential duration and downregulation of several K(+) currents might participate in the genesis of arrhythmias associated with chronic heart failure. Little information is available related to the mechanism by which TNF-alpha modulates cardiac K(+) channels. The present study analyzes the effect of TNF-alpha on the transient outward K(+) current (I(to)) in rat ventricular myocytes, using the whole cell patch-clamp technique. We found that TNF-alpha is able to induce a significant reduction of I(to) density, modifies its inactivation, and downregulates the Kv4.2 protein expression, while calcium current density is not affected. We have also demonstrated that the reduction of I(to) density induced by TNF-alpha was prevented by the selective inducible nitric oxide synthase (iNOS) inhibitor 1400-W, the protein synthesis inhibitor cycloheximide, the antioxidant tocopherol, and the superoxide dismutase mimetic manganese(III) tetrakis (4-benzoic acid) porphyrin. In addition, a reduced I(to) density was recorded in ventricular myocytes exposed to peroxynitrite, supporting a possible participation of this oxidant in the effects of TNF-alpha on I(to). We conclude that TNF-alpha exposure, through iNOS induction and generation of oxidant species, promotes electrophysiological changes (decreased I(to) and action potential duration prolongation) in rat ventricular myocytes, providing new insights into how cytokines modulate K(+) channels in the heart. 相似文献
999.
Ramírez-Sotelo G López-Bayghen E Hernández-Kelly LC Arias-Montaño JA Bernabé A Ortega A 《Neurochemical research》2007,32(1):73-80
Appropriate removal of l-glutamate from the synaptic cleft is important for prevention of the excitotoxic effects of this neurotransmitter. The Na+-dependent glutamate/aspartate transporter GLAST is regulated in the short term, by a transporter-dependent decrease in uptake
activity while in the long term, a receptor’s-dependent decrease in GLAST protein levels leads to a severe reduction in glutamate
uptake. The promoter region of the mouse glast gene harbors an Activator Protein-1 site (AP-1). To gain insight into the molecular mechanisms triggered by Glu-receptors
activation involved in GLAST regulation, we took advantage of the neonatal mouse cerebellar prisms model. We characterized
the glutamate uptake activity; the glutamate-dependent effect on GLAST protein levels and over the interaction of nuclear
proteins with a mouse glast promoter AP-1 probe. A time and dose dependent decrease in transporter activity matching with a decrease in GLAST levels
was recorded upon glutamate treatment. Moreover, a significant increase in glast AP-1 DNA binding was found. Pharmacological experiments established that both effects are mediated through α-amino-3-hydroxy-5-methyl-4-isoxazole
propionate receptors, favoring the notion of the critical involvement of glutamate in the regulation of its binding partners:
receptors and transporters. 相似文献
1000.
Romero-Avila M de Dios-Bravo G Mendez-Stivalet JM Rodríguez-Sotres R Iglesias-Arteaga MA 《Steroids》2007,72(14):955-959
Two furostanic analogues of brassinosteroids bearing the 5alpha-hydroxy-6-oxo moiety were synthesized and their biological activity studied using the bean second internode elongation test. One of the compounds produced significant stimulation at doses of 2.5 and 5ng/plant. 相似文献