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991.
Yang Ou Shang-Jui Wang Le Jiang Bin Zheng Wei Gu 《The Journal of biological chemistry》2015,290(1):457-466
Although p53 is frequently mutated in human cancers, about 80% of human melanomas retain wild-type p53. Here we report that PHGDH, the key metabolic enzyme that catalyzes the rate-limiting step of the serine biosynthesis pathway, is a target of p53 in human melanoma cells. p53 suppresses PHGDH expression and inhibits de novo serine biosynthesis. Notably, upon serine starvation, p53-mediated cell death is enhanced dramatically in response to Nutlin-3 treatment. Moreover, PHGDH has been found recently to be amplified frequently in human melanomas. We found that PHGDH overexpression significantly suppresses the apoptotic response, whereas RNAi-mediated knockdown of endogenous PHGDH promotes apoptosis under the same treatment. These results demonstrate an important role of p53 in regulating the serine biosynthesis pathway through suppressing PHGDH expression and reveal serine deprivation as a novel approach to sensitize p53-mediated apoptotic responses in human melanoma cells. 相似文献
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Qing-Yin Wang Hongping Dong Bin Zou Ratna Karuna Kah Fei Wan Jing Zou Agatha Susila Andy Yip Chao Shan Kim Long Yeo Haoying Xu Mei Ding Wai Ling Chan Feng Gu Peck Gee Seah Wei Liu Suresh B. Lakshminarayana CongBao Kang Julien Lescar Francesca Blasco Paul W. Smith Pei-Yong Shi 《Journal of virology》2015,89(16):8233-8244
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Jing Guo Hongjuan He Qi Liu Fengwei Zhang Jie Lv Tiebo Zeng Ning Gu Qiong Wu 《Molecules and cells》2015,38(10):859-865
Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5–E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct. 相似文献
998.
Yi-Ting Huang Jia-Teh Liao Li-Chen Yen Yung-Kun Chang Yi-Ling Lin Ching-Len Liao 《Journal of biomedical science》2015,22(1)
Background
To construct safer recombinant flavivirus vaccine, we exploited Japanese encephalitis virus (JEV) replicon-based platform to generate single-round infectious particles (SRIPs) that expressed heterologous neutralizing epitope SP70 derived from enterovirus-71 (EV71). Such pseudo-infectious virus particles, named SRIP-SP70, although are not genuine viable viruses, closely mimic live virus infection to elicit immune responses within one round of viral life cycle.Results
We found that, besides gaining of full protection to thwart JEV lethal challenge, female outbred ICR mice, when were immunized with SRIP-SP70 by prime-boost protocol, could not only induce SP70-specific and IgG2a predominant antibodies but also provide their newborns certain degree of protection against EV71 lethal challenge.Conclusions
Our results therefore exemplify that this vaccination strategy could indeed confer an immunized host a dual protective immunity against subsequent lethal challenge from JEV or EV71. 相似文献999.
Androgen receptor binding to an androgen‐responsive element in the promoter of the Srsf4 gene inhibits its expression in mouse Sertoli cells
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