排序方式: 共有30条查询结果,搜索用时 15 毫秒
21.
A self-review of long-standing studies of induced androgenesis in fish. The data are provided on the induction of diploid androgenesis in teleost fish via blocking the first cleavage division and the development of the method of dispermic androgenesis in sturgeon fish. Androgenetic nucleocytoplasmic hybrids were obtained using the method of dispermic androgenesis and eggs of previously produced true interspecific hybrids. Specific morphological features of androgenetic hybrids were described and dispermic androgenesis was realized using cryopreserved sperm. The mechanism of sex determination in sturgeon fish is discussed with reference to possible us of androgenesis for restoration of rare and disappearing species. 相似文献
22.
It has been shown that the effects of stimulation of germination of wheat seeds by electromagnetic field depend on the degree of membrane tension during imbibition of seeds in sucrose solutions. This provides further confirmation of the influence of electromagnetic fields on the release of proteins from the bound state on the membranes. The prolonged treatment with electromagnetic fields during the imbibition of seeds leads not only to the inhibition of germination of sprouts but also to a decrease in their germinability, which can be as strong as twofold for seeds with the initial low germinability. This is related to the desynchronization of germination processes, caused by the stimulation of the release of proteins and inhibition of another stage during the cell division, the assembly of complex structures. It is noted that the activation of the release of proteins and inhibition of their binding by the action of electromagnetic fields must elevate the cell excitability. The presumably, the excitability of cells determines the effects of magnetic storms and high solar activity on the physiological state of organisms. 相似文献
23.
A. S. Grunina A. V. Rekoubratsky L. I. Tsvetkova A. E. Barmintseva E. D. Vasil’eva K. V. Kovalev O. G. Poluektova 《Russian Journal of Developmental Biology》2011,42(2):108-119
Dispermic androgenesis was used to produce, for the first time, an androgenetic progeny of the Siberian sturgeon (Acipenser baerii) and the androgenetic nucleo-cytoplasmic hybrids (Siberian sturgeon, A. baerii × Russian sturgeon, A. gueldenstaedtii) using cryopreserved sperm. Microsatellite DNA analysis confirmed exclusively paternal inheritance in the androgenetic progeny
of Siberian sturgeon. Heterozygotes for certain microsatellite loci were detected among the androgenetic hybrids, thereby
confirming a dispermic nature of androgenesis. According to the data of comparative morphological analysis, the obtained androgenetic
hybrid, by the age of 15 months old, was completely identical to the paternal species. Both a female and a male were detected
in the androgenetic sturgeon progenies, which is of interest for producing bisexual progenies via androgenesis. The data of
this study confirm the feasibility of dispermic androgenesis using cryopreserved sperm to preserve and recover the gene pools
of endangered sturgeon species. 相似文献
24.
25.
RNase has been isolated from the homogenate of the Aspergillus clavatus mycelium by gel filtration through Sephadex G-75, chromatography on CM-cellulose and DEAE-cellulose. By gel filtration and electrophoresis in polyacrylamide gel the preparation has been shown to be homogeneous. The enzyme is acid protein with the isoelectric point at pH 4.4 and molecular weight of 27,000. RNase has pH optimum at 6.0--6.2 and temperature optimum 60 degrees for RNA action. The enzyme splits RNA completely in the absence of metal ions. Ions Zn2+, Cu+2, Ag+1 and Ni+2 at a concentration of 10(-4) M are strong inhibitors of RNase activity. 相似文献
26.
A self-review of long-standing studies of induced androgenesis in fish. The data are provided on the induction of diploid androgenesis in teleost fish via blocking the first cleavage division and the development of the method of dispermic androgenesis in sturgeon fishes. Androgenetic nucleocytoplasmic hybrids were obtained using the method of dispermic androgenesis and eggs of previously produced true interspecific hybrids. Specific morphological features of androgenetic hybrids were described and dispermic androgenesis was realized using cryopreserved sperm. The mechanism of sex determination in sturgeon fishes is discussed with reference to possible use of androgenesis for restoration of rare and disappearing species.__________Translated from Ontogenez, Vol. 36, No. 4, 2005, pp. 254–264.Original Russian Text Copyright © 2005 by Grunina, Recoubratsky. 相似文献
27.
Karyagina Anna S. Gromov Alexander V. Grunina Tatyana M. Lyaschuk Alexander M. Grishin Alexander V. Strukova Natalia V. Generalova Maria S. Galushkina Zoya M. Soboleva Lyubov’ A. Dobrinina Olga Yu. Bolshakova Tatyana N. Subbotina Marina E. Romanovskaya-Romanko Ekaterina A. Krasilnikov Igor V. Polyakov Nikita B. Solovyev Andrey I. Grumov Daniil A. Zhukhovitsky Vladimir G. Ryabova Ekaterina I. Prokofiev Vladimir V. Lunin Vladimir G. 《Biochemistry. Biokhimii?a》2021,86(10):1275-1287
Biochemistry (Moscow) - A new platform for creating anti-coronavirus epitope vaccines has been developed. Two loop-like epitopes with lengths of 22 and 42 amino acid residues were selected from the... 相似文献
28.
29.
E. O. Osidak M. S. Osidak D. E. Sivogrivov T. S. Portnaya T. M. Grunina L. A. Soboleva V. G. Lunin A. S. Karyagina S. P. Domogatskii 《Applied Biochemistry and Microbiology》2014,50(2):200-205
The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collagen hydrogel in the presence of human blood plasma have been studied. The expulsion of rhBMP-2 from the collagen-BMP-2 complex by the competitive adhesion of collagen-binding proteins penetrating from plasma was firstly recognized. It was experimentally proven that that blood plasma fibronectin is the main collagen-binding protein, which is responsible for the controlled release of rhBMP-2. As a result, a new collagen hydrogel with the incorporation of fibronectin was created which retained rhBMP-2 for a twice longer period as compared to the ordinary collagen hydrogel. A distinctive feature of this new collagen-fibronectin matrix is the slow release of rhBMP-2 in the first three days which allows for the avoiding of adverse effects in clinics caused by the rapid release of large amounts of rhBMP-2 from collagen hydrogel. 相似文献
30.
Stimulation of wheat seed germination by brief exposure in a 50-Hz electromagnetic field (EMF) is shown to depend on the extent of membrane stretching upon seed swelling in sucrose solutions. This again confirms the effect of low-frequency EMF on the release of peripheral proteins from membranes. Prolonged exposure, on the contrary, decreases the germination rate and retards seedling growth. 相似文献