Immunological study of the cellular components of Pseudomonas aeruginosa. VI. The toxicity and protective properties of its mucus and its fractions]

Capsule-like mucus was obtained from two newly isolated mucoid strains of P. aeruginosa and then treated with ethanol. The mucus was fractionated by the method of differential centrifugation (at 15, 000 g for 1 h, at 105, 000 or 170, 000 g for 3 h) and gel chromatography in columns packed with Sepharose 4B. The sediment fractions contained 30--80% of high molecular polysaccharide protein (peptide) mucus components which were toxic for mice and protected 25--77% of rats against experimental P. aeruginosa infection. The supernatant fluid fractions contained 60--80% of predominantly protein components with molecular mass between 20,000 and 60,000 daltons. These mucus components were slightly toxic for mice and rats and protected 80--100% of rats against P. aeruginosa infection.     

Immunologic study of the cellular components of bacillus pyocyaneus. III. Immunochemical analysis, toxicity and protective properties of water-soluble antigenic complexes]

Aqueous extracts of two Ps. aeruginosa strains killed with acetone were subjected to fractionation by preparative ultracentrifugation and gel-chromatography. Toxic activity of the extract was found to be connected with the high-molecular, possibly glycoprotein components reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 30--40% of rats against the generalized infection with Pseudomonas aeruginosa. This protective activity is apparently connected with the protein components (molecular weight--20000--60000 dalton), nontoxic for mice, not reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 60 to 80% of rats against Ps. aeruginosa infecsion. Thus, as a result of preparative ultracentrifugation and gel-chromatography it was postible to divide the toxic and the nontoxic protective components of Ps. aeruginosa.     

A study of conformational states of substrates of isoform 3A4 of cytochrome P450

A multiconformational study of substrates for isoform 3A4 of cytochrome P450 with the use of the BiS/MC algorithm has been carried out. The use of this approach made it possible to determine the pseudo-atomic model of this cytochrome and to find the substrate conformers responsible for binding to the cytochrome. It was found that in most cases, the geometry of the conformer, which is bound to the isoform, substantially differs from the geometry of the global minimum conformer. It was shown that, as a rule, the mirror antipodes (enantioconformers) are characterized by different Michaelis constants. The quantitative relationship of the Michaelis constants with the parameters of interaction in the model complexes between the isoform 3A4 and substrates was determined. This dependence describes an experimental value of the Michaelis constant with a squared cross-validation correlation coefficient of 0.88, which was determined by leave-one-out cross-validation technique.     

Immunologic study of artificial complex antigens obtained from P. aeruginosa lipopolysaccharides]

Artificial antigens were obtained on the basis of the polysaccharide component of P. aeruginosa complexed with an indifferent protein. Immunological study indicated that the specific polysaccharide of P. aeruginosa lipopolysaccharide contained two structures, high molecular and low molecular, having qualitative and quantitative differences in their hydrocarbon composition. Artificial complex antigens possessed serological and immunogenic properties, the low molecular polysaccharide fraction complexed with protein having less pronounced serological and immunogenic activity than polysaccharide and the high molecular fraction complexed with protein. Antificial complex antigens exerted no protective effect in generalized P. aeruginosa infection in rats.     

Spatial and typological organization of the oribatid mite population in the Northeastern Altai

Major features of the spatial heterogeneity of the oribatid mite population in the Northeastern Altai are revealed from the data obtained in the field seasons of 2002 and 2006. The impact of environmental factors on the population appearance is evaluated.     

Hypertrophy of the adrenal cortex

The human adrenal cortex in essential hypertension and in the salt-losing form of the adrenogenital syndrome and the adrenal cortex in spontaneously hypertensive rats were studied by morphometry. Under long-term functional loading hypertrophy of adrenocortical cells is the common way of increasing the mass of the cortex. The correlation between the morphological parameters of the adrenocortical structures increases in this condition. Hyperplasia along with hypertrophy has been found in man at an early age. The differences in the degree of hypertrophy of the nuclei and nucleoli may be connected with different intensity of adrenocortical function in different pathological conditions.