Suppression of the immune response to ovalbumin in vivo using anti-idiotypic antibodies

The effect of anti-idiotype antibodies (a-IdpI) on primary immune response to ovalbumin (OVA) was studied. A-IdpI against OVA antibodies were obtained with pI 6.2-6.7 from BALB/c mice. About 30-40% of IgG plaque cell formation (PCF) was inhibited if a-IdpI antiserum was added in situ, which served as a test for the evaluation of idiotype-positive (Id+) PCF. Up to 70% of common PCF and 70-80% of Id+ PCF were suppressed in mice that were injected with a-IdpI antibodies prior to immunization. This suppression was antigen- and allo-specific and depended upon the time of a-IdpI injection. If a-IdpI antibodies were disaggregated (DA) the Id+ suppression increased. A-IdpI antibodies also decreased IgE response to OVA, the suppression being most pronounced with the use of DA samples.     

Neuroimmunoendocrine interaction in the pathogenesis of chronic heart failure

Chronic heart failure (CHF) is a common complication of patients with heart dysfunction and of those suffering from various chronic illnesses. Although recently developed therapies revolutionized treatment of CHF, life expectancy of the survivors is still significantly reduced. Proposed neurohoromonal approach in CHF treatment is on the whole rather unsatisfactory. Recently the interest of clinical investigators has been focused on immune mechanisms in the pathogenesis of CHF. In this review we tried to summarize data concerning contribution of inflammatory cytokines to pathogenesis of CHF. The source and site of action of pro- and antiinflammatory cytokines and number of interactions among cytokines and neuroendocrine systems under CHF are considered. Finally we discuss novel therapies managing correction of both immune and neurohormonal status of the patient.     

Localization of Mechanisms of Amplitude–Frequency Modulation of Pulse Blood Perfusion of the Soft Tissue Microvasculature. Pilot Study

Doklady Biochemistry and Biophysics - Peripheral blood flow analysis is used for noninvasive assessment of the cardiovascular system. The aim was to investigate the possibility of applying...     

Atemonate and Imuteran: novel Russian monoclonal antibody-based therapeutic agents

The N. Blokhin National Cancer Research Center is one of the few Russian scientific institutions in which hybridoma technology of monoclonal antibody (mAb) production has been successfully established. Using this technology, several dozens of mAbs to various antigens of human leukocytes have been elaborated. These mAbs are widely used for immune status evaluation and for differential diagnostics of leukemias. Two mAbs were used to develop therapeutic drugs. Imuteran is a pharmaceutical form of mAb ICO-25 against a mucin-like antigen of human milk fat globules and proposed for treatment of epithelial cell-originating cancers (breast, intestinal, ovarian, lung cancer, etc.). ThePhase II clinical study of this agent is now nearly completed, and preliminary results suggest Imuteran to be a promising anticancer agent with tumor-stabilizing activity, but patients should be carefully monitored for signs of allergic reactions. mAb ICO-90 against the CD3 antigen of human T lymphocytes was used to develop the therapeutic agent Atemonate proposed for treatment of acute transplant rejection. At present, the Phase II clinical study of this agent is over, and the results confirm the drug safety and efficacy for this indication. The drug is being registered at the Ministry of Healthcare and Social Development, and transfer to serial production is expected shortly.     

The Impact of Stochastic Perturbations upon the Hydrodynamic Relationship between the Activity of Human Cardiac Ventricles and Low-Frequency Blood Flow Oscillations in the Microcirculatory Bed

Biophysics - Abstract—The experimentally revealed high phase coherence between low-frequency oscillations in the cutaneous blood perfusion signal at the contralateral skin sites indicates the...     

Slow Ca2'-binding Mg2+- EDTA buffer for intracellular perfusion

In studies of currents in perfused cells or their membrane fragments containing potential-dependent Ca channels and Ca2+-activated channels, a buffer slowly binding Ca2+ appears useful in some cases. A buffer with EDTA in excess of Mg2+ has been proposed, and its kinetic characteristics have been calculated. It has been shown that this buffer, depending on its component proportions, may provide Ca2+ binding with a characteristic time of up to tens of milliseconds. For comparison, in the cytoplasm, this time does not exceed 1 ms even with a large calcium signal, as follows from calculations.     

The status of the endocrine function of the thymus in acute radiation sickness resulting from the accident at the Chernobyl Nuclear Power Station

Data are presented on the role of inhibition of endocrine function of the thymus in the pathogenesis of acute radiation sickness resulted from the direct and indirect (via the increased glucocorticoid production) effects of ionizing radiation. The complex treatment, including nonspecific active immunotherapy, permitted to normalize the thymic hormone level and certain parameters of the immune system.