全文获取类型
收费全文 | 227篇 |
免费 | 18篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2019年 | 2篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 9篇 |
2013年 | 8篇 |
2012年 | 12篇 |
2011年 | 8篇 |
2010年 | 7篇 |
2009年 | 3篇 |
2008年 | 9篇 |
2007年 | 12篇 |
2006年 | 10篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 8篇 |
2002年 | 5篇 |
2001年 | 9篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 6篇 |
1989年 | 1篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 5篇 |
1975年 | 8篇 |
1974年 | 8篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 6篇 |
排序方式: 共有245条查询结果,搜索用时 15 毫秒
81.
Oxygen Consumption Rates and Oxygen Concentration in Molt-4 Cells and Their mtDNA Depleted (ρ0) Mutants
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jiangang Shen Nadeem Khan Lionel D. Lewis Ray Armand Oleg Grinberg Eugene Demidenko Harold Swartz 《Biophysical journal》2003,84(2):1291-1298
Respiratory deficient cell lines are being increasingly used to elucidate the role of mitochondria and to understand the pathophysiology of mitochondrial genetic disease. We have investigated the oxygen consumption rates and oxygen concentration in wild-type (WT) and mitochondrial DNA (mtDNA) depleted (ρ0) Molt-4 cells. Wild-type Molt-4 cells have moderate oxygen consumption rates, which were significantly reduced in the ρ0 cells. PCMB (p-chloromercurobenzoate) inhibited the oxygen consumption rates in both WT and ρ0 cells, whereas potassium cyanide decreased the oxygen consumption rates only in WT Molt-4 cells. Menadione sodium bisulfite (MSB) increased the oxygen consumption rates in both cell lines, whereas CCCP (carbonyl cyanide m-chlorophenylhydrazone) stimulated the oxygen consumption rates only in WT Molt-4 cells. Superoxide radical adducts were observed in both WT and ρ0 cells when stimulated with MSB. The formation of this adduct was inhibited by PCMB but not by potassium cyanide. These results suggest that the reactive oxygen species (ROS) induced by MSB were at least in part produced via a mitochondrial independent pathway. An oxygen gradient between the extra- and intracellular compartments was observed in WT Molt-4 cells, which further increased when cells were stimulated by CCCP and MSB. The results are consistent with our earlier findings suggesting that such oxygen gradients may be a general phenomenon found in most or all cell systems under appropriate conditions. 相似文献
82.
V N Vasil'chenko S M Grinberg S V Samuleva T V Stoliarova E S Skobel'tsyna 《Radiobiologiia》1974,14(5):715-719
83.
The steady-state viscosity (η*) of the liquid two-phase disperse system water-casein-sodium alginate of varying composition, with a disperse particle diameter of about 30 μm, has been investigated in the shear rate range
. The flow curves obtained are similar in shape. They are invariant in composition and can be fitted to the flow curve equation
where η*0 is the Newtonian viscosity obtained from data at low shear stresses and k is an empirical parameter. Both η*0 and k depend on the composition of the two-phase system.The experimental dependence of the viscosity on the composition follows a logarithmic additivity law at high shear stresses, but at low shear stresses the observed viscosities are lower than would be predicted by this law. The experimental dependence of the ratio
(the mean activation volume) on the composition is also not in agreement with the additivity law. These special features of the steady-state flow of the investigated disperse system are explained by the presence of a low-concentration interphase layer having a lower viscosity and less pronounced non-Newtonian behaviour than the disperse and continuous phases. This interphase layer exists in other two-phase systems which consist of a solvent and two incompatible polymers. For this reason, the rheological behaviour of the system investigated may be expected to be common for two-phase systems of this type, particularly protein-polysaccharide mixtures in water. 相似文献
84.
Summary Autoradiographic investigation of the cell cycle of 12 diploid and 12 abnormal human fibroblast strains was carried out. Two cell strains (trisomy 7 and monosomy 21) derived from spontaneous abortuses showed prolongation of the G2 period accompanied by the shortening of the S period. Other cytogenetically abnormal embryonic strains (trisomy 9, 14 and triploidy) did not deviate from the diploid pattern. Three cell strains (LHC-1-70, LHC-6-70, LHC-411) derived from the patients with karyotypes 47,XXX, 47,XY,+18 and 46,XX,5p—respectivly had embryonic types of proliferation with a short G2 period. In two other strains from the patients with Down's syndrom the G2 period was prolonged. There was no statistically significant difference in the parameters of the cell cycle between the control and the strains derived from a patient with Klinefelter syndrom and from a male patient with karyotype 46,XX. The modificatory effect of the chromosomal abnormalities on the parameters of cell cycle is discussed.
Zusammenfassung Der Zellcyclus von 12 normal diploiden und 12 abnormen menschlichen Fibroblasten-Zellinien wurde untersucht. Zwei Zellinien (Trisomie 7 und Monosomie 21) zeigten eine Verlängerung der G2-Phase und eine Verkürzung der S-Phase. Zellinien (Trisomie 9, 14 und Triploidie) wichen nicht von der Norm ab. Drei Zellinien (47,XXX; 47,XY,+18; 46,XX,5p-) zeigten eine Verkürzung von G2. G2 war dagegen verlängert bei zwei Zellinien von Down-Syndrom. Keine Abweichung fand sich bei einem Klinefelter-Patienten und einem männlichen Probanden mit 46,XX.相似文献
85.
Offen D Gilgun-Sherki Y Barhum Y Benhar M Grinberg L Reich R Melamed E Atlas D 《Journal of neurochemistry》2004,89(5):1241-1251
Increasing evidence suggests that enhanced production of reactive oxygen species (ROS) activates the MAP kinases, c-Jun N-terminal protein kinase (JNK) and mitogen-activated protein kinase MAPK (p38). These phosphorylated intermediates at the stress-activated pathway induce expression of matrix metalloproteinases (MMPs), leading to inflammatory responses and pathological damages involved in the etiology of multiple sclerosis (MS). Here we report that N-acetylcysteine amide (AD4) crosses the blood-brain barrier (BBB), chelates Cu(2+), which catalyzes free radical formation, and prevents ROS-induced activation of JNK, p38 and MMP-9. In the myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, oral administration of AD4 drastically reduced the clinical signs, inflammation, MMP-9 activity, and protected axons from demylination damages. In agreement with the in vitro studies, we propose that ROS scavenging by AD4 in MOG-treated animals prevented MMP's induction and subsequent damages through inhibition of MAPK pathway. The low toxicity of AD4 coupled with BBB penetration makes this compound an excellent potential candidate for the therapy of MS and other neurodegenerative disorders. 相似文献
86.
Genetic ablations of iron regulatory proteins 1 and 2 reveal why iron regulatory protein 2 dominates iron homeostasis 总被引:15,自引:0,他引:15
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
87.
Diaz-Font A Cormand B Santamaria R Vilageliu L Grinberg D Chabás A 《Human genetics》2005,117(2-3):275-277
Only two Gaucher disease (GD) patients bearing mutations in the prosaposin gene (PSAP), and not in the glucocerebrosidase gene (GBA), have been reported. In both cases, one mutant allele remained unidentified. We report here the identification of the second mutation in one of these patients, being the first complete genotype described so far in a SAP-C-deficient GD patient. This mutation, p.Q430X, is the first one reported in the saposin D domain and probably produces a null allele by nonsense mediated mRNA decay. 相似文献
88.
Diana Valverde Teresa Solans Daniel Grinberg Susana Balcells Lluisa Vilageliu Mónica Bayés Pilar Chivelet Claude Besmond Michel Goossens Roser González-Duarte Montserrat Baiget 《Human genetics》1996,97(1):35-38
We report the molecular analysis of the subunit of the rod phosphodiesterase (PDEB) gene in a consanguineous autosomal recessive retinitis pigmentosa family that shows homozygosity for polymorphisms in the genomic region comprising this gene, and positive linkage between a PDEB marker and the diesease. The two affected sisters are homozygous for a T to G transversion in codon 699 of the PDEB gene, leading to the substitution of a leucine by an arginine residue. This change, enclosed in the catalytic domain of the PDEB, could result in a modification of the protein structure preventing the physiological hydrolysis of cGMP. 相似文献
89.
A V Grinberg N M Gevondyan N V Grinberg V Y Grinberg 《European journal of biochemistry》2001,268(19):5027-5036
The thermal unfolding and domain structure of Na+/K+-ATPase from pig kidney were studied by high-sensitivity differential scanning calorimetry (HS-DSC). The excess heat capacity function of Na+/K+-ATPase displays the unfolding of three cooperative domains with midpoint transition temperatures (Td) of 320.6, 327.5, 331.5 K, respectively. The domain with Td = 327.5 K was identified as corresponding to the beta subunit, while two other domains belong to the alpha subunit. The thermal unfolding of the low-temperature domain leads to large changes in the amplitude of the short-circuit current, but has no effect on the ATP hydrolysing activity. Furthermore, dithiothreitol or 2-mercaptoethanol treatment causes destruction of this domain, accompanied by significant disruption of the ion transporting function and a 25% loss of ATPase activity. The observed total unfolding enthalpy of the protein is rather low (approximately 12 J.g-1), suggesting that thermal denaturation of Na+/K+-ATPase does not lead to complete unfolding of the entire molecule. Presumably, transmembrane segments retain most of their secondary structure upon thermal denaturation. The binding of physiological ligands results in a pronounced increase in the conformational stability of both enzyme subunits. 相似文献
90.
Leonid N. Grinberg Oded Shalev Ada Goldfarb Eliezer A. Rachmilewitz 《生物化学与生物物理学报:疾病的分子基础》1992,1139(3):248-250
Primaquine, a prooxidant antimalarial drug, incubated with human red blood cells (RBC) induced marked superoxide generation in the cells as detected by exogenous cytochrome c reduction. In the presence of primaquine, β-thalassemic RBC produced significantly more superoxide than normal RBC, thus reflecting the vulnerability of β-thalassemic cells to oxidative stress. 相似文献