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51.
Varyukhina S Freitas M Bardin S Robillard E Tavan E Sapin C Grill JP Trugnan G 《Microbes and infection / Institut Pasteur》2012,14(3):273-278
Rotaviruses attach to intestinal cells in a process that requires glycan recognition. Some bacteria from the gut microflora have been shown to modify cell-surface glycans. In this study, human intestinal cultured cells were incubated with bacteria-derived soluble factors and infected with rotavirus. Results show that only bacterial soluble factors that increase cell-surface galactose namely, those of Bacteroides thetaiotaomicron and Lactobacillus casei were able to efficiently block rotavirus infections. Increasing cell-surface galactose using galactosyltransferase resulted in a similar blockage of rotavirus infections. These results indicate that manipulation of cell-surface intestinal glycans by bacterial soluble factors can prevent rotavirus infection in a species-specific manner, and should now be considered a potential therapeutic approach against rotavirus infection. 相似文献
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Electrical stimulation of the central nervous system creates both orthodromically propagating action potentials, by stimulation of local cells and passing axons, and antidromically propagating action potentials, by stimulation of presynaptic axons and terminals. Our aim was to understand how antidromic action potentials navigate through complex arborizations, such as those of thalamic and basal ganglia afferents-sites of electrical activation during deep brain stimulation. We developed computational models to study the propagation of antidromic action potentials past the bifurcation in branched axons. In both unmyelinated and myelinated branched axons, when the diameters of each axon branch remained under a specific threshold (set by the antidromic geometric ratio), antidromic propagation occurred robustly; action potentials traveled both antidromically into the primary segment as well as "re-orthodromically" into the terminal secondary segment. Propagation occurred across a broad range of stimulation frequencies, axon segment geometries, and concentrations of extracellular potassium, but was strongly dependent on the geometry of the node of Ranvier at the axonal bifurcation. Thus, antidromic activation of axon terminals can, through axon collaterals, lead to widespread activation or inhibition of targets remote from the site of stimulation. These effects should be included when interpreting the results of functional imaging or evoked potential studies on the mechanisms of action of DBS. 相似文献
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Butterfly response to severe ENSO-induced forest fires in Borneo 总被引:1,自引:0,他引:1
Abstract. 1. Little is known about animal community response to severe El Niño Southern Oscillation (ENSO)-induced fire events. Here the response of butterflies to the 1997/98 ENSO-induced fire event in East Kalimantan (Indonesian Borneo) is assessed. In addition to the community-wide study, a detailed assessment of the lycaenid Jamides celeno is made.
2. Species richness declined significantly from 211 species pre-ENSO to 39 species post-ENSO and community composition changed significantly. Along with the decline in species richness there was a marked increase in dominance. Jamides celeno , for example, increased from 3% of the pre-ENSO assemblage to 58% of the post-ENSO assemblage. Like J. celeno , most of the species in the post-ENSO assemblage were generalists; most of the specialist species having disappeared from pre- to post-ENSO.
3. The major host plant used by J. celeno was the abundant resprouting Fordia splendidissima . Furthermore, significantly more eggs were laid on plants with the crazy ant, Anoplolepis gracilipes , present than on plants with other ants or no ant attendance. Caterpillar presence was significantly higher on plants tended by ants than on untended plants.
4. The median distance moved by J. celeno was 30 m with a maximum recorded distance of 290 m.
5. The abundance of J. celeno and other generalists in the post-ENSO assemblage at Wanariset was probably related to their ability to utilise the few available resources after the fire (e.g. F. splendidissima resprouts), their presence in degraded habitats surrounding the Wanariset forest, and their ability to disperse successfully by either being strong fliers (e.g. Euploea spp.) or being able to attain very high population sizes and thereby produce a surplus of dispersers (e.g. J. celeno ). 相似文献
2. Species richness declined significantly from 211 species pre-ENSO to 39 species post-ENSO and community composition changed significantly. Along with the decline in species richness there was a marked increase in dominance. Jamides celeno , for example, increased from 3% of the pre-ENSO assemblage to 58% of the post-ENSO assemblage. Like J. celeno , most of the species in the post-ENSO assemblage were generalists; most of the specialist species having disappeared from pre- to post-ENSO.
3. The major host plant used by J. celeno was the abundant resprouting Fordia splendidissima . Furthermore, significantly more eggs were laid on plants with the crazy ant, Anoplolepis gracilipes , present than on plants with other ants or no ant attendance. Caterpillar presence was significantly higher on plants tended by ants than on untended plants.
4. The median distance moved by J. celeno was 30 m with a maximum recorded distance of 290 m.
5. The abundance of J. celeno and other generalists in the post-ENSO assemblage at Wanariset was probably related to their ability to utilise the few available resources after the fire (e.g. F. splendidissima resprouts), their presence in degraded habitats surrounding the Wanariset forest, and their ability to disperse successfully by either being strong fliers (e.g. Euploea spp.) or being able to attain very high population sizes and thereby produce a surplus of dispersers (e.g. J. celeno ). 相似文献
55.
Hydrogen peroxide is a regulator of ABI1, a protein phosphatase 2C from Arabidopsis. 总被引:8,自引:0,他引:8
Protein phosphatases 2C (PP2Cs) exhibit diverse regulatory functions in signalling pathways of animals, yeast and plants. ABI1 is a PP2C of Arabidopsis that exerts negative control on signalling of the phytohormone abscissic acid (ABA). Characterisation of the redox sensitivity of ABI1 revealed a strong enzymatic inactivation by hydrogen peroxide (H2O2) which has recently been implicated as a secondary messenger of ABA signalling. H2O2 reversibly inhibited ABI1 activity in vitro with an IC(50) of approximately 140 microM in the presence of physiological concentrations of glutathione. In addition, ABI1 was highly susceptible to inactivation by phenylarsine oxide (IC(50)=3-4 microM) indicative for the facile oxidation of vicinal cysteine residues. Thus, H2O2 generated during ABA signalling seems to inactivate the negative regulator of the ABA response. 相似文献
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Sulphate uptake and xylem loading was analysed in young pea (Pisum sativum) seedlings. The rate of sulphate uptake into intact 8-days-old pea seedlings (determined by a 1 h exposure to radiolabelled sulphate in the nutrient solution) was 585 nmol sulphate g–1 root fresh weight h–1. When the cotyledons were removed on day 6 the 8-days-old seedlings took up only 7% of the controls. Interruption of the phloem transport by steam girdling of the stem or the root (1 h before incubation with radiolabelled sulphate) diminished sulphate uptake by approximately 50%. The addition of sucrose to the nutrient solution during incubation did not restore sulphate uptake rates indicating that the decrease was not due to a lack of energy. Apparently, a signal from the shoot and/or the cotyledons is necessary to stimulate sulphate uptake into the roots of pea seedlings. Glutathione fed to the roots for 3 h prior to incubation with radiolabelled sulphate diminished sulphate uptake by approximately 50%. The relative proportion of the sulphate taken up that was loaded into the xylem remained unchanged (between 7 and 9% of total uptake), even when the stem was girdled above the cotyledons or when the seedlings were pre-exposed to glutathione. Only removal of the cotyledons or girdling of the root below the cotyledons increased the proportion of sulphate loaded into the xylem to 13–15% of total uptake upon exposure to glutathione. Apparently, a signal from the cotyledons represses xylem loading to some extent. 相似文献
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