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41.
目的:研究卡托普利对早期高血压大鼠动脉血管平滑肌细胞内质网相关因子葡萄糖调节蛋白78(glucose-regulated protein of 78kd,GRPT8)和C/EBP同源蛋白(CAAT/enhancer binding protein homologous protein,CHOP)表达的影响.方法:将18只成年雄性Sprague-Dawley(SD)大鼠随机分为对照组、模型组和卡托普利组(n=6),模型组和卡托普利组均采用大鼠腹主动脉结扎建立高血压大鼠模型.4周后测量血压,应用免疫组化方法检测主动脉血管平滑肌细胞GRP78和CHOP的表达;缺口末端标记法(TUNEL)检测细胞凋亡.结果:(1)模型组平均动脉压(mean arterial blood pressure,MAP)明显增加,卡托普利组血压低于模型组,高于对照组,差异具有统计学意义(P<0.05);(2)模型组内质网因子GRP78、CHOP表达均增加,卡托普利组GRP78、CHOP表达低于模型组,高于对照组,差异具有统计学意义(P<0.01);(3)模型组血管平滑肌细胞凋亡率减少,卡托普利组血管平滑肌细胞凋亡率高于模型组,低于对照组,差异具有统计学意义(P<0.01).结论:卡托普利可降低高血压大鼠动脉血管平滑肌细胞GRP78和CHOP的表达,增加细胞凋亡.可能与其减弱高血压所致内质网反应,维持血管平滑肌细胞的增殖/凋亡平衡有关. 相似文献
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Teplova VV Tonshin AA Grigoriev PA Saris NE Salkinoja-Salonen MS 《Journal of bioenergetics and biomembranes》2007,39(4):321-329
Novel activities of bafilomycin A1, a macrolide antibiotic known as an inhibitor of V-ATPases, were discovered. Bafilomycin
A1 induced uptake of potassium ions by energized mitochondria and caused mitochondrial swelling, loss of membrane potential,
uncoupling of oxidative phosphorylation, inhibition of the maximal respiration rates, and induced pyridine nucleotide oxidation.
The mitochondrial effects provoked by nanomolar concentrations of bafilomycin A1 were connected to its activity as a potent,
K+-specific ionophore. The K+ ionophoric activity of bafilomycin A1 was observed also in black lipid membranes, indicating that it was an inherent property
of the bafilomycin A1 molecule. It was found that bafilomycin A1 is a K+ carrier but not a channel former. Bafilomycin A1 is the first and currently unique macrolide antibiotic with K+ ionophoric properties. The novel properties of bafilomycin A1 may explain some of the biological effects of this plecomacrolide
antibiotic, independent of V-ATPase inhibition. 相似文献
45.
大黄鱼对几种饲料蛋白原料消化率的研究 总被引:12,自引:0,他引:12
以白鱼粉为主要蛋白源制成参考饲料,以0.1%的Cr2O3为外源性指示剂,按参考饲料和实验原料70%∶30%的比例配制成实验饲料,测定了大黄鱼(Pseudosciaena crocea)对白鱼粉、红鱼粉、肉骨粉、豆粕、花生粕、菜籽粕和棉籽粕的表观消化率。实验大黄鱼(平均初始体重15.0±1.6g)养殖于海水浮式网箱(1.5m×1.5m×2.0m)中,分别以各实验饲料投喂实验鱼5周,然后采用挤压法收集粪便。实验结果表明各原料表观干物质消化率在43.6%至70.0%之间,能量消化率在42.9%到82.6%的范围内。实验原料的蛋白质表观消化率在70.7%到92.4%之间,其中白鱼粉和红鱼粉的蛋白消化率最高(分别为92.4%和89.3%),而棉籽粕则最低(70.7%)。脂肪的表观消化率在61.3%到90.5%之间,其中棉籽粕最低(61.3%)。磷的表观消化率相对较低,仅白鱼粉和红鱼粉在53.2%以上,其余皆低于37.5%。总之,大黄鱼对这几种原料的表观消化率存在较大差异,但蛋白消化率均较高,因此,可作为大黄鱼的饲料蛋白源在实际饲料配制中使用。 相似文献
46.
鹿蹄草植物的研究与应用 总被引:1,自引:0,他引:1
对鹿蹄草植物的自然资源分布、化学成分、药理作用、及其应用的相关研究做了综述。该植物在世界各地广泛分布、含有鹿蹄草素等多种化学成分,药理研究证明其具有抗菌、抗病毒、消炎、镇痛作用、对心脑血管系统等作用,主要应用在医药工业、食品工业和园林绿化面。最后对该属植物的研究前景做了展望。 相似文献
47.
Belyaev IY Grigoriev YG 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2007,47(6):727-732
Since pioneering investigations published in the beginning of 1970th, various biological responses to non-thermal (NT) microwaves (MW), including adverse health effects, have been described by many research groups all over the world. There is strong evidence that the NT MW biological effects depend on several physical parameters and biological variables, which must be controlled in replication studies. Apart from the fundamental importance, the development of comprehensive mechanisms for the NT MW effects is socially important. The effects of MW of mobile communications are of major concern because of the increased exposure in many countries. It has been shown that adverse effects of NT MW from GSM/UMTS mobile phones on human lymphocytes from healthy and hypersensitive to EMF persons depend on carrier frequency and modulation. Further investigations with human primary cells, animals and volunteers are needed to elucidate possible adverse effects of MW signals that are used in wireless communication. Identification of those types and frequency channels/bands for mobile communication, which do not affect human cells, is urgently needed as the high priority task for the development of safe mobile communication. Numerous data on the NT MW effects clearly indicate that the SAR-concept alone cannot underlie the safety guidelines for chronic exposures to MW from mobile communication and other approaches are needed. However, there is not enough research information to set exposure MW standards. Various genetic and epigenetic effects of signals used in mobile communication should be studied. It has been shown that NT MW affect cells of various types including stem cells and reproductive organs. Stem cells represent especially important cellular model because recent data suggest that different cancer types, including leukemia, have a fundamentally common basis that is grounded on epigenetic changes in stem cells. 相似文献
48.
本文旨在通过观察过氧化氢酶(catalase,CAT)抑制剂氨基三唑(aminotriazole,ATZ)对酒精诱导的急性肝损伤的影响,初步探讨CAT在酒精性肝损伤中的可能作用。以雄性Sprague Dawley(SD)大鼠为实验对象,采用酒精腹腔注射诱导急性肝损伤,在造模前30 min腹腔注射不同剂量ATZ(100~400 mg/kg)或相同体积溶剂(对照)。造模24 h后,检测大鼠血浆天冬氨酸转氨酶(aspartate transaminase,AST)、丙氨酸转氨酶(alanine transaminase,ALT)及乳酸脱氢酶(lactate dehydrogenase,LDH)水平,用HE染色法观察肝组织病理改变程度,用试剂盒检测肝组织内CAT活性、过氧化氢(hydrogen peroxide,H2O2)水平及丙二醛(malondialdehyde,MDA)含量,ELISA法检测血浆中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白介素-6(interleukin-6,IL-6)水平。结果显示,ATZ处理可剂量依赖性降低酒精暴露大鼠血浆中ALT、AST及LDH水平,并减轻酒精诱导的肝组织病理损伤;ATZ可抑制酒精暴露大鼠肝组织内CAT活性、降低H2O2水平及MDA含量;ATZ也可下调酒精暴露大鼠血浆中TNF-α和IL-6水平。以上结果表明,ATZ可减轻酒精诱导的大鼠急性肝损伤,提示CAT可能在酒精性肝损伤中发挥了重要的致病作用。 相似文献
49.
Genetic isolation between two recently diverged populations of a symbiotic fungus 总被引:1,自引:0,他引:1 下载免费PDF全文
Sara Branco Pierre Gladieux Christopher E. Ellison Alan Kuo Kurt LaButti Anna Lipzen Igor V. Grigoriev Hui‐Ling Liao Rytas Vilgalys Kabir G. Peay John W. Taylor Thomas D. Bruns 《Molecular ecology》2015,24(11):2747-2758
Fungi are an omnipresent and highly diverse group of organisms, making up a significant part of eukaryotic diversity. Little is currently known about the drivers of fungal population differentiation and subsequent divergence of species, particularly in symbiotic, mycorrhizal fungi. Here, we investigate the population structure and environmental adaptation in Suillus brevipes (Peck) Kuntze, a wind‐dispersed soil fungus that is symbiotic with pine trees. We assembled and annotated the reference genome for Su. brevipes and resequenced the whole genomes of 28 individuals from coastal and montane sites in California. We detected two clearly delineated coast and mountain populations with very low divergence. Genomic divergence was restricted to few regions, including a region of extreme divergence containing a gene encoding for a membrane Na+/H+ exchanger known for enhancing salt tolerance in plants and yeast. Our results are consistent with a very recent split between the montane and coastal Su. brevipes populations, with few small genomic regions under positive selection and a pattern of dispersal and/or establishment limitation. Furthermore, we identify a putatively adaptive gene that motivates further functional analyses to link genotypes and phenotypes and shed light on the genetic basis of adaptive traits. 相似文献
50.
利用氮离子注入对链霉菌的诱变效应,筛选高产恩拉霉素的变异菌株。利用不同剂量的氮离子对杀真菌放线菌S.fungicidicus NL629-3菌株进行诱变处理,研究低能氮离子注入对其存活率及产恩拉霉素能力的影响。低能氮离子注入剂量在60×1013ions/cm2时对链霉菌的诱变效应显著,试验得到了5株恩拉霉素产量较高的突变菌株,其中N3-643菌株经连续传代4次,遗传稳定性较好,其摇瓶发酵水平较对照提高了41%,放大发酵生产后平均发酵水平提高25.8%。离子注入诱变是获得高产恩拉霉素突变菌株的有效方法。 相似文献