全文获取类型
收费全文 | 545篇 |
免费 | 27篇 |
国内免费 | 9篇 |
出版年
2022年 | 5篇 |
2020年 | 5篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2015年 | 11篇 |
2014年 | 8篇 |
2013年 | 16篇 |
2012年 | 15篇 |
2011年 | 20篇 |
2010年 | 20篇 |
2009年 | 22篇 |
2008年 | 18篇 |
2007年 | 15篇 |
2006年 | 17篇 |
2005年 | 9篇 |
2004年 | 16篇 |
2003年 | 9篇 |
2002年 | 6篇 |
2001年 | 8篇 |
1999年 | 12篇 |
1998年 | 12篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1983年 | 6篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1978年 | 5篇 |
1974年 | 5篇 |
1972年 | 5篇 |
1970年 | 4篇 |
1969年 | 5篇 |
1959年 | 8篇 |
1958年 | 24篇 |
1957年 | 26篇 |
1956年 | 26篇 |
1955年 | 22篇 |
1954年 | 22篇 |
1953年 | 13篇 |
1952年 | 13篇 |
1951年 | 10篇 |
1950年 | 9篇 |
排序方式: 共有581条查询结果,搜索用时 281 毫秒
101.
Staphylococcus aureus is a leading cause of septicaemia and infective endocarditis. The overall incidence of staphylococcal bacteraemia is increasing, contributing to 16% of all hospital-acquired bacteraemias. The use of cardiac pacemakers has revolutionized the management of rhythm disturbances, yet this has also resulted in a group of patients at risk of pacemaker lead endocarditis and seeding in the range of 1% to 7%. We describe a 26-year-old man with transposition of the great arteries who had a pacemaker implanted and presented with S. aureus septicaemia 2 years postpacemaker implantation and went on to develop pacemaker lead endocarditis. This report illustrates the risk of endocarditis in the population with congenital heart disease and an intracardiac device. 相似文献
102.
103.
研究抗肿瘤药阿霉素对Wnt通路抑制因子FrpHE(frizzled-related protein)和DKK-1(Dickkopf-1)表达的作用.将抗肿瘤药阿霉素加入到人肝癌HepG2(HepG2,含野生型p53;Hep3B,p53缺失)、人大肠癌(Lovo,含野生型p53)和人神经胶质瘤细胞(U251,p53突变)细胞株中.以RT-PCR技术检测阿霉素对Wnt通路抑制因子FrpHE和DKK-1的表达调节作用,以流式细胞术检测在肿瘤细胞中Wnt通路的关键调节因子β-catenin的表达.在加入阿霉素24h后FrpHEmRNA表达水平在人肝癌细胞(HepG2,含野生型p53;Hep3B,p53缺失)中与对照组相比表达水平显著增加.在人大肠癌细胞(Lovo,含野生型p53)和人神经胶质瘤细胞(U251,p53突变型)细胞中,未见FrpHE mRNA表达.DKK-1mRNA表达水平在人肝癌细胞(HepG2,含野生型p53;Hep3B,p53缺失)、人大肠癌细胞(Lovo,含野生型p53)和人神经胶质瘤细胞(U251,p53突变型)中与对照组相比表达水平显著增加.β-catenin的阳性细胞百分比强度和平均荧光量强度与对照组相比,表达水平降低.提示化疗药阿霉素能明显诱导抑制剂FrpHEmRNA和DKK-1mRNA的表达. 相似文献
104.
New epidemiological features on animal trypanosomiasis by molecular analysis in the pastoral zone of Sideradougou, Burkina Faso 总被引:2,自引:0,他引:2
T. LEFRANÇOIS P. SOLANO S. DE LA ROCQUE† Z. BENGALY J. M. REIFENBERG I. KABORE & D. CUISANCE 《Molecular ecology》1998,7(7):897-904
A multidisciplinary work was undertaken in the agropastoral zone of Sidéradougou, Burkina Faso to try to elucidate the key factors determining the presence of tsetse flies. In this study the PCR was used to characterize trypanosomes infecting the vector ( Glossina tachinoides and Glossina palpalis gambiensis ) and the host, i.e. cattle. A 2-year survey involved dissecting 2211 tsetse of the two Glossina species. A total of 298 parasitologically infected tsetse were analysed by PCR. Trypanosoma vivax was the most frequently identified trypanosome followed by the savannah type of T. congolense and, to a lesser extent, the riverine forest type of T. congolense , and by T. brucei . No cases of T. simiae were found. From the 107 identified infections in cattle, the taxa were the same, but T. congolense savannah type was more frequent, whereas T. vivax and T. congolense riverine forest types were found less frequently. A correlation was found between midgut infection rates of tsetse, nonidentified infections and reptile bloodmeals. These rates were higher in G.p. gambiensis , and in the western part of the study area. T. vivax infections were related to cattle bloodmeals, and were more frequent in G. tachinoides and in the eastern study area. The PCR results combined with bloodmeal analysis helped us to establish the relationships between the vector and the host, to assess the trypanosome challenge in the two parts of the area, to elucidate the differences between the two types of T. congolense , and to suspect that most midgut infections were originating from reptilian trypanosomes. 相似文献
105.
106.
107.
A. G. DE LA FILIA S. ANDREWES J. M. CLARK L. ROSS 《Medical and veterinary entomology》2018,32(2):226-234
Insect reproduction is extremely variable, but the implications of alternative genetic systems are often overlooked in studies on the evolution of insecticide resistance. Both ecotypes of Pediculus humanus (Phthiraptera: Pediculidae), the human head and body lice, are human ectoparasites, the control of which is challenged by the recent spread of resistance alleles. The present study conclusively establishes for the first time that both head and body lice reproduce through paternal genome elimination (PGE), an unusual genetic system in which males transmit only their maternally derived chromosomes. Here, we investigate inheritance patterns of parental genomes using a genotyping approach across families of both ecotypes and show that heterozygous males exclusively or preferentially pass on one allele only, whereas females transmit both in a Mendelian fashion. We do however observe occasional transmission of paternal chromosomes through males, representing the first known case of PGE in which whole‐genome meiotic drive is incomplete. Finally, we discuss the potential implications of this finding for the evolution of resistance and invite the development of new theoretical models of how this knowledge might contribute to increasing the success of pediculicide‐based management schemes. 相似文献
108.
Fruit morphology in Tragopogon L. (Compositae: Lactuceae) from the Iberian Peninsula 总被引:2,自引:0,他引:2
G. BLANCA C. DÍAZ DE LA GUARDIA 《Botanical journal of the Linnean Society. Linnean Society of London》1997,125(4):319-329
Achenes of all the Tragopogon species from the Iberian Peninsula were examined by means of scanning-electron microscopy and stereomicroscopy. The achenes of the eight species are described, illustrated and compared. The results are contrasted with the systematics of this genus. The isolated position of T. lamottei with regard to the other seven species is noted. A key is provided to enable the different species to be distinguished. 相似文献
109.
First record in America of Aedes albopictus naturally infected with dengue virus during the 1995 outbreak at Reynosa, Mexico 总被引:2,自引:0,他引:2
SERGIO IBÁÑEZ-BERNAL BALTASAR BRISEÑO JOHN PAUL MUTEBI ENID ARGOT GUADALUPE RODRÍGUEZ CARMEN MARTINEZ-CAMPOS RAFAEL PAZ PEDRO DE LA FUENTE-SAN ROMÁN† ROBERTO TAPIA-CONYER‡ ANA FLISSER 《Medical and veterinary entomology》1997,11(4):305-309
Abstract Mosquito collections were conducted during a dengue outbreak in Reynosa, Tamaulipas, Mexico, July-December 1995. A total of 6694 adult mosquitoes (four genera and nine species) were captured, of which 2986 (78.3% females and 21.7% males) were Aedes albopictus and 2339 (39.7% females and 60.3% males) were Ae.aegypti. These two species comprised 84.2% of the total collection. Specimens were grouped into pools, nearly 50% of them processed for detection of virus by cythopathic effect in C6-36 and VERO cell cultures and by haemagglutination test. Five pools gave positive haemagglutin-ation reactions and were examined by immunofluorescence using monoclonal antibodies to flavivirus and to dengue virus. One pool of ten Ae.albopictus males was positive for dengue virus: serotypes 2 and 3 were identified by serotype-specific monoclonal antibodies arid confirmed by RT-PCR. This is the first report of Ae.albopictus naturally infected with dengue virus in America. Also, it is the very first time Ae.albopictus males have been found infected with dengue virus in the wild. 相似文献
110.