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991.
Gamma-ray-induced numerical and structural chromosome anomalies in mouse immature oocytes 总被引:1,自引:0,他引:1
Young female mice were given 1, 2 or 3 Gy of chronic gamma-irradiation. Metaphase II oocytes from these mice were sampled 8 weeks after the end of the treatment and screened for numerical and structural chromosome anomalies. The proportions of hyperhaploid (n + 1) metaphase II oocytes increased after 1 and 2 Gy (significantly after the latter) but remained at the control level after 3 Gy of gamma-rays. Structural chromosome anomalies were significantly increased above control levels at all doses and also showed an increase with dose to 2 Gy and a decline at 3 Gy. The cause of this unusual dose-response pattern for induced chromosome damage is uncertain. These results show that significant chromosome damage can be induced by irradiation of immature oocytes, a cell stage previously suggested to be resistant to induced genetic damage. 相似文献
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Robin D. Rogers Andrew H. Bond Cary B. Bauer Jianhua Zhang Scott T. Griffin 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1996,680(1-2):221-229
Solvent extraction, utilizing an oil-water mixture (e.g, chloroform-water) and a suitable complexant, is a proven technology for the selective removal and recovery of metal ions from aqueous solutions. Aqueous biphasic systems (ABS), formed by mixing certain inorganic salts and water-soluble polymers, or by mixing two dissimilar water-soluble polymers, have been studied for more than 40 years for the gentle, non-denaturing separation of fragile biomolecules, yet ABS have been virtually ignored as a possible extraction technology for metal ions. In this report we review our metal ion partitioning work and discuss the three major types of partitioning: (1) those rare instances that the metal ion species present in a given solution partitions to the PEG-rich phase without an extractant; (2) the use of halide salts which produce a metal anion complex that partitions to the PEG-rich phase; and (3) the use of a water-soluble extractant which distributes to the PEG-rich phase. In addition, we correlate the partitioning behavior we observed with available thermodynamic data for metal ions and their complexes. 相似文献
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NADPH, nicotinamide adenine dinucleotide phosphate (reduced form); EPR, electron paramagnetic resonance; MNP, 2-methyl-2-nitrosopropanePreliminary accounts of this work were presented at the American Chemical Society Southeast—Southwest Regional Meeting, October 29–31, 1975, Memphis, TN and at the American Chemical Society Southwest Regional Meeting, December 5–7, 1977, Little Rock, AR 相似文献
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