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61.
Molecular evolution modeled as a fractal Poisson process in agreement with mammalian sequence comparisons 总被引:1,自引:1,他引:0
The fractal doubly stochastic Poisson process (FDSPP) model of molecular
evolution, like other doubly stochastic Poisson models, agrees with the
high estimates for the index of dispersion found from sequence comparisons.
Unlike certain previous models, the FDSPP also predicts a positive
geometric correlation between the index of dispersion and the mean number
of substitutions. Such a relationship is statistically proven herein using
comparisons between 49 mammalian genes. There is no characteristic rate
associated with molecular evolution according to this model, but there is a
scaling relationship in rates according to a fractal dimension of
evolution. The FDSPP is a suitable replacement for the homogeneous Poisson
process in tests of the lineage dependence of rates and in estimating
confidence intervals for divergence times. As opposed to other fractal
models, this model can be interpreted in terms of Darwinian selection and
drift.
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62.
We describe the upper portion of the bill sheath (rhinotheca) of the kākāpō (Strigops habroptilus) from three adult female specimens. The external buccal surface of the rhinotheca is deeply concave with a prominent palatal stop and hardened chevrons creating a ‘milling apparatus’ that the kākāpō uses to grind food. The palatal stop presents a working face of 40–50?mm2. The internal surface of the rhinotheca mirrors the overlying premaxilla and provides a distinct thickened abutment consistent with resistance against the increased workload of the mandibles (gnathotheca) due to the kākāpō’s fibrous diet and chewing style. Along the midline, the rhinotheca at the abutment is up to 5.6?mm thick, compared with as thin as 2.1?mm elsewhere on the midline. The closely related Nestor parrots have less developed palatal stops, chevrons and abutments on their rhinothecas consistent with their lower preference for fibrous plant material. The form of the rhinotheca agrees with the kākāpō’s feeding ecology as a generalist herbivore that grinds locally available fibrous material to assist digestion. 相似文献
63.
From a panel of 24 alleged antioxidants the most suitable antioxidants (AO) for use with chemiluminescence (CL) experiments were determined. Superoxide dismutase (SOD), using luminol as the chemiluminescence probe (Lum-CL), was inhibitory only towards O2*- and not HO* or (1)O2. SOD was thus a suitable antioxidant for O2*-, as was tiron. Tiron had advantages, however, since SOD acted as a pro-oxidant in the presence of H2O2 or H2O2/HO* generators. The two most suitable antioxidants for (1)O2 were diphenylisobenzofuran (DBF) and tryptophan, for both Lum and Lucigenin-CL (Luc-CL). Desferrioxamine, with both Lum and Luc-CL, was a very effective scavenger for HO*, but appeared to be an even more effective scavenger for (1)O2. Cysteamine showed the best discrimination between IC50s when the two (1)O2 generators NaOCl/H2O2 and NDPO2 were compared. Cysteamine was, therefore, the only scavenger that was appropriate for studies with hypochlorite. Melatonin, with Lum-CL, was found to be the most suitable scavenger for HO*. Mannitol, the classical AO for HO*, was not suitable when used with CL since it acted as a pro-oxidant. Some of the AOs revealed either calyx- or bell-shaped CL inhibition profiles and presumably, therefore, may act as both pro- or antioxidants at different concentrations. Antioxidants showing these kinds of dual activities should be used with caution in CL studies. 相似文献
64.
WS Watkins R Thara BJ Mowry Y Zhang DJ Witherspoon W Tolpinrud MJ Bamshad S Tirupati R Padmavati H Smith D Nancarrow C Filippich LB Jorde 《BMC genetics》2008,9(1):1-17
Background
Major population movements, social structure, and caste endogamy have influenced the genetic structure of Indian populations. An understanding of these influences is increasingly important as gene mapping and case-control studies are initiated in South Indian populations.Results
We report new data on 155 individuals from four Tamil caste populations of South India and perform comparative analyses with caste populations from the neighboring state of Andhra Pradesh. Genetic differentiation among Tamil castes is low (RST = 0.96% for 45 autosomal short tandem repeat (STR) markers), reflecting a largely common origin. Nonetheless, caste- and continent-specific patterns are evident. For 32 lineage-defining Y-chromosome SNPs, Tamil castes show higher affinity to Europeans than to eastern Asians, and genetic distance estimates to the Europeans are ordered by caste rank. For 32 lineage-defining mitochondrial SNPs and hypervariable sequence (HVS) 1, Tamil castes have higher affinity to eastern Asians than to Europeans. For 45 autosomal STRs, upper and middle rank castes show higher affinity to Europeans than do lower rank castes from either Tamil Nadu or Andhra Pradesh. Local between-caste variation (Tamil Nadu RST = 0.96%, Andhra Pradesh RST = 0.77%) exceeds the estimate of variation between these geographically separated groups (RST = 0.12%). Low, but statistically significant, correlations between caste rank distance and genetic distance are demonstrated for Tamil castes using Y-chromosome, mtDNA, and autosomal data.Conclusion
Genetic data from Y-chromosome, mtDNA, and autosomal STRs are in accord with historical accounts of northwest to southeast population movements in India. The influence of ancient and historical population movements and caste social structure can be detected and replicated in South Indian caste populations from two different geographic regions. 相似文献65.
AG. van Ginkel BJ. Sorgdrager M. A. de Graaf I. Karalis N. Ajmone Marsan 《Netherlands heart journal》2014,22(2):77-79
We report a case of an allergic reaction after the administration of an echocardiographic contrast agent which resulted in ST-segment elevation. Hypersensitivity and allergic reactions are known causes of acute cardiovascular events. However, only limited reports are available which suggest the exact mechanism of the occurrence of angina or myocardial infarction during severe allergic reactions. In our case, through invasive imaging (coronary angiography and IVUS) we have shown for the first time a transient coronary spasm in the absence of intra-coronary thrombus and only minimal neointimal hyperplasia. 相似文献
66.
PEKKA VANHALA KRISTIINA KARHU MIKKO TUOMI KATARINA BJÖRKLÖF HANNU FRITZE HASSE HYVÄRINEN JARI LISKI 《Global Change Biology》2011,17(1):538-550
Changes in soil carbon, the largest terrestrial carbon pool, are critical for the global carbon cycle, atmospheric CO2 levels and climate. Climate warming is predicted to be most pronounced in the northern regions and therefore the large soil carbon pool residing in boreal forests will be subject to larger global warming impact than soil carbon pools in the temperate or the tropical forest. A major uncertainty in current estimates of the terrestrial carbon balance is related to decomposition of soil organic matter (SOM). We hypothesized that when soils are exposed to warmer climate the structure of the ground vegetation will change much more rapidly than the dominant tree species. This change will alter the quality and amount of litter input to the soil and induce changes in microbial communities, thus possibly altering the temperature sensitivity of SOM decomposition. We transferred organic surface soil sections from the northern borders of the boreal forest zone to corresponding forest sites in the southern borders of the boreal forest zone and studied the effects of warmer climate after an adaptation period of 2 years. The results showed that initially ground vegetation and soil microbial community structure and community functions were different in northern and southern forest sites and that 2 years of exposure to warmer climate was long enough to cause changes in these ecological indicators. The rate of SOM decomposition was approximately equally sensitive to temperature irrespective of changes in vegetation or microbial communities in the studied forest sites. However, as temperature sensitivity of the decomposition increases with decreasing temperature regime, the proportional increase in the decomposition rate in northern latitudes could lead to significant carbon losses from the soils. 相似文献
67.
Lissette M. Ruberté Raghu N. Natarajan Gunnar BJ. Andersson 《Journal of biomechanics》2009,42(3):341-348
The current study investigated mechanical predictors for the development of adjacent disc degeneration. A 3-D finite element model of a lumbar spine was modified to simulate two grades of degeneration at the L4–L5 disc. Degeneration was modeled by changes in geometry and material properties. All models were subjected to follower preloads of 800 N and moment loads in the three principal directions of motion using a hybrid protocol. Degeneration caused changes in the loading and motion patterns of the segments above and below the degenerated disc. At the level (L3–L4) above the degenerated disc, the motion increased due to moderate degeneration by 21% under lateral bending, 26% under axial rotation and 28% under flexion/extension. At the level (L5-S1) below the degenerated disc, motion increased only during lateral bending by 20% due to moderate degeneration. Both the L3–L4 and L5-S1 segment showed a monotonic increase in both the maximum von Mises stress and shear stress in the annulus as degeneration progressed for all loading directions, expect extension at L3–L4. The most significant increase in stress was observed at the L5-S1 level during axial rotation with nearly a ten-fold increase in the maximum shear stress and 103% increase in the maximum von Mises stress. The L5-S1 segment also showed a progressive increase in facet contact force for all loading directions with degeneration. Nucleus pressure did not increase significantly for any loading direction at either the caudal or cephalic adjacent segment. Results suggest that single-level degeneration can increase the risk for injury at the adjacent levels. 相似文献
68.
Twenty-four Boer goat does were used to compare three superovulation protocols, with 8 does allocated per treatment during the natural breeding season. In Group 1 (Day 0 protocol), the oestrous cycles of does were synchronised for 7 days with CIDR's and injected PGF2α at CIDR insertion. Does were then superovulated with pFSH in 7 dosages at 12 h intervals, starting 88 h following CIDR removal. Concurrently with the 6th dosage, does were injected PGF2α. Cervical inseminations were performed 24 h and 36 h following the last superovulatory treatment. For Groups 2 and 3, the oestrous cycles of the does were also synchronised for 17 days using CIDR's. On day 14 of CIDR insertion, Group 2 does were injected with PGF2α. A superovulation treatment similar to Group 1 was administered in Groups 2 and 3, starting 48 h before CIDR removal. All does in these groups were also cervically inseminated with fresh undiluted Boer goat semen 24 h and 36 h following CIDR withdrawal. Embryos from all 3 treatment groups were flushed on day 6 following AI. Does in Group 1 responded to the short oestrous synchronisation protocol before the administration of a superovulation treatment (71.4% response), with time to onset of oestrus of 37.2 ± 0.7 h and duration of an induced oestrous period of 36.4 ± 0.5 h being recorded. Following superovulation only two does exhibited signs of oestrus in Group 1, while Groups 2 and 3 exhibited a 100% oestrous response. Groups 1 and 2 recorded similar intervals to the onset and durations of the induced oestrous period. The number of ovulations per donor was significantly lower in Group 1 (4.0 ± 0.7), compared to Groups 2 and 3 (14.5 ± 0.6 and 16.5 ± 0.8, respectively), with no significant difference between Groups 2 and 3. The Day 0 protocol (Group 1) also resulted into a significantly lower total number of structures recovered, compared to Group 3. Groups 2 and 3 recorded a relatively similar number of structures recovered. The number of embryos recovered was significantly lower (P < 0.01) in Group 1 (0.2 ± 0.1) than in Group 2 (13.2 ± 0.5) and Group 3 (11.5 ± 1.1), with the mean number of unfertilised ova and degenerated embryos being similar for all 3 treatment groups. Groups 2 and 3 also produced a similar number of transferable embryos. The blood progesterone concentrations followed a similar trend in the 3 treated groups, from CIDR insertion to embryo flushing. However, the mean serum progesterone concentration was significantly lower on day 4 in the Day 0 group, compared to Groups 2 and 3. The inclusion of PGF2α treatment in the superovulation protocol for Boer goats had no beneficial effect, while the Day 0 protocol engaged in this trial, resulted in a lower superovulation response. Further research is warranted, focusing on synchronisation, time when initiating superovulatory treatment and AI to improve the embryo yield in goats. 相似文献
69.
The oligomerization of a family of four genetically clustered human gastrointestinal mucins 总被引:1,自引:0,他引:1
Mucins are synthesized and secreted by many epithelia. They are complex
glycoproteins that offer cytoprotection. In their functional configuration,
mucins form oligomers by a biosynthetic process that is poorly understood.
A family of four human gastrointestinal mucin genes (MUC2, MUC5AC, MUC5B,
and MUC6) is clustered to chromosome 11p15.5. To study oligomerization of
these related mucins, we performed metabolic labeling experiments with
[35S]amino acids in LS174T cells, and isolated mucin precursors by specific
immunoprecipitations that were analyzed on SDS-PAGE. Each of the precursors
of MUC2, MUC5AC, MUC5B, and MUC6 formed a single species of
disulfide-linked homo-oligomer within 1 h after pulse labeling. Based on
apparent molecular masses, these oligomeric precursors were most likely
dimers. Inhibition of vesicular RER-to-Golgi transport, with brefeldin A
and CCCP, did not affect the dimerization of MUC2 precursors, localizing
dimerization to the RER. O-Glycosylation of MUC2 followed dimerization.
Inhibition of N- glycosylation by tunicamycin retarded, but did not
inhibit, dimerization, indicating that N-glycans play a role in efficient
dimerization of MUC2 precursors. Based on sequence homology, the ability of
MUC2, MUC5AC, MUC5B and MUC6 to dimerize most likely resides in their
C-terminal domains. Thus, the RER-localized dimerization of secretory
mucins likely proceeds by similar mechanisms, which is an essential step in
the formation of the human gastrointestinal mucus- gels.
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70.
1. Considerable evidence suggests that the diversity within plant communities may strongly affect the strength of species interactions, but the majority of studies only considered interspecific diversity. 2. This paper examines the effect of intraspecific genetic diversity within Brassica fields on two Brassica specialists, cabbage root fly, and diamondback moth, and on a parasitoid attacking diamondback moths. Genetic diversity was manipulated both in a replacement and an additive design. 3. Both herbivore densities and parasitism rates were higher in smaller plots, with limited responses to increased within‐plot diversity. All species showed variable densities across genotypes, and preference hierarchies were species specific. 4. Responses to plot size in root flies scaled with the diameter‐to‐area ratio, suggesting that patch detectability affected local density, whereas responses by diamondback moths and parasitoids deviated from this ratio. These species differences could be traced to differences in the residence time within patches, where diamondback moths typically spend longer and more variable time periods in patches than root flies. 5. The lack of response to genetic diversity by both herbivores suggests that egg‐laying rates are affected by decisions on the plant and not by attraction from a distance, neither to the plant itself nor the patch. Patterns of differential attack may then be due to different acceptability for studied genotypes. 6. Future theories on insect responses to spatial heterogeneity should focus on species traits and how traits interact with information landscapes in the field. 相似文献