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41.
Effect of coexposure to 50 Hz magnetic fields and an aneugen on human lymphocytes,determined by the cytokinesis block micronucleus assay 总被引:3,自引:0,他引:3
Interference of 50 Hz extremely low frequency magnetic fields (ELF-MF) with the known aneugen vinblastine (VBL) on micronucleus formation was tested with the in vitro cytokinesis block micronucleus assay in human lymphocyte cultures. Isolated lymphocyte cultures were prepared from 18 individuals. Three groups of quadruplicate cultures from six unrelated individuals were exposed to 50 Hz ELF-MF of background (bkg), 80 and 800 microT, respectively, during the complete incubation period (72 h). Twenty-four hours after culture initiation, one replicate culture from each individual within each ELF-MF group was exposed to 0, 5, 10, or 15 ng/ml VBL. The isolated lymphocyte cultures were scored for the presence of micronuclei, the nuclear division index (NDI), and apoptosis. As expected, increased VBL concentration resulted in an increased micronucleus and apoptosis frequency and in a decreased NDI. In the presence of VBL, there was a systematic tendency for increased micronucleus and apoptosis frequency in the ELF-MF exposed groups compared to the bkg group. In the absence of VBL, we observed no statistically significant effect of ELF-MF on micronucleus induction or apoptosis frequency, but the NDI was significantly higher in the 800 microT group compared to the other groups, suggesting an effect of ELF-MF on cell proliferation. An interaction between ELF-MF and VBL on NDI was observed. This interaction reflected the drastic decrease in NDI due to coexposure to VBL. 相似文献
42.
43.
We assessed the distribution and prevalence of growth anomalies (GAs) in Acropora from French Frigate Shoals (Hawaii, USA), Johnston Atoll and Tutuila (American Samoa), developed a nomenclature for gross morphology, characterized GAs at the cellular level and obtained preliminary indices of their spatial patterns and progression within coral colonies. Acropora GAs were found in all 3 regions, but the distribution, variety and prevalence of Acropora GAs was highest in American Samoa. GAs were grouped into 7 gross morphologies (exophytic, bosselated, crateriform, nodular, vermiform, fimbriate or annular). On histology, GAs consisted of hyperplastic basal body wall (calicodermis, mesoglea and gastrodermis apposed to skeleton) with 3 distinct patterns of necrosis. There was no evidence of anaplasia or mitotic figures (common but not necessarily required morphologic indicators of neoplasia). Compared to normal tissues, GAs had significantly fewer polyps, zooxanthellae within the gastrodermis of the coenenchyme, mesenterial filaments and gonads but significantly more necrosis. On 2 colonies with GAs monitored at 2 points over 11 mo, numbers of GAs per colony increased from 0.9 to 3 times the original number seen, and significant clustering of GAs occurred within colonies. The evidence of GAs being true neoplasias (tumors) is mixed, so a cautionary approach is urged in use of morphologic terminology. 相似文献
44.
45.
Introduction
Obesity is linked to type 2 diabetes and risk factors associated to the metabolic syndrome. Consumption of dietary fibres has been shown to have positive metabolic health effects, such as by increasing satiety, lowering blood glucose and cholesterol levels. These effects may be associated with short-chain fatty acids (SCFAs), particularly propionic and butyric acids, formed by microbial degradation of dietary fibres in colon, and by their capacity to reduce low-grade inflammation.Objective
To investigate whether dietary fibres, giving rise to different SCFAs, would affect metabolic risk markers in low-fat and high-fat diets using a model with conventional rats for 2, 4 and 6 weeks.Material and Methods
Conventional rats were administered low-fat or high-fat diets, for 2, 4 or 6 weeks, supplemented with fermentable dietary fibres, giving rise to different SCFA patterns (pectin – acetic acid; guar gum – propionic acid; or a mixture – butyric acid). At the end of each experimental period, liver fat, cholesterol and triglycerides, serum and caecal SCFAs, plasma cholesterol, and inflammatory cytokines were analysed. The caecal microbiota was analysed after 6 weeks.Results and Discussion
Fermentable dietary fibre decreased weight gain, liver fat, cholesterol and triglyceride content, and changed the formation of SCFAs. The high-fat diet primarily reduced formation of SCFAs but, after a longer experimental period, the formation of propionic and acetic acids recovered. The concentration of succinic acid in the rats increased in high-fat diets with time, indicating harmful effect of high-fat consumption. The dietary fibre partly counteracted these harmful effects and reduced inflammation. Furthermore, the number of Bacteroides was higher with guar gum, while noticeably that of Akkermansia was highest with the fibre-free diet. 相似文献46.
Variation in parent–offspring kinship in socially monogamous systems with extra‐pair reproduction and inbreeding 下载免费PDF全文
Jane M. Reid Greta Bocedi Pirmin Nietlisbach A. Bradley Duthie Matthew E. Wolak Elizabeth A. Gow Peter Arcese 《Evolution; international journal of organic evolution》2016,70(7):1512-1529
Female extra‐pair reproduction in socially monogamous systems is predicted to cause cuckolded socially‐paired males to conditionally reduce paternal care, causing selection against extra‐pair reproduction and underlying polyandry. However, existing models and empirical studies have not explicitly considered that cuckolded males might be related to their socially‐paired female and/or to her extra‐pair mate, and therefore be related to extra‐pair offspring that they did not sire but could rear. Selection against paternal care, and hence against extra‐pair reproduction, might then be weakened. We derive metrics that quantify allele‐sharing between within‐pair and extra‐pair offspring and their mother and her socially‐paired male in terms of coefficients of kinship and inbreeding. We use song sparrow (Melospiza melodia) paternity and pedigree data to quantify these metrics, and thereby quantify the joint effects of extra‐pair reproduction and inbreeding on a brood's total allelic value to its socially‐paired parents. Cuckolded male song sparrows were almost always detectably related to extra‐pair offspring they reared. Consequently, although brood allelic value decreased substantially following female extra‐pair reproduction, this decrease was reduced by within‐pair and extra‐pair reproduction among relatives. Such complex variation in kinship within nuclear families should be incorporated into models considering coevolutionary dynamics of extra‐pair reproduction, parental care, and inbreeding. 相似文献
47.
Lander Baeten Margot Vanhellemont Pieter De Frenne An De Schrijver Martin Hermy Kris Verheyen 《Oecologia》2010,163(4):1021-1032
The differential ability of forest herbs to colonize secondary forests on former agricultural land is generally attributed
to different rates of dispersal. After propagule arrival, however, establishing individuals still have to cope with abiotic
soil legacies from former agricultural land use. We focused on the plastic responses of forest herbs to increased phosphorus
availability, as phosphorus is commonly found to be persistently bioavailable in post-agricultural forest soils. In a pot
experiment performed under field conditions, we applied three P levels to four forest herbs with contrasting colonization
capacities: Anemone nemorosa, Primula elatior, Circaea lutetiana and Geum urbanum. To test interactions with light availability, half of the replicas were covered with shade cloths. After two growing seasons,
we measured aboveground P uptake as well as vegetative and regenerative performance. We hypothesized that fast-colonizing
species respond the most opportunistically to increased P availability, and that a low light availability can mask the effects
of P on performance. All species showed a significant increase in P uptake in the aboveground biomass. The addition of P had
a positive effect on the vegetative performances of two of the species, although this was unrelated to their colonization
capacities. The regenerative performance was affected by light availability (not by P addition) and was related to the species’
phenology. Forest herbs can obviously benefit from the increased availability of P in post-agricultural forests, but not all
species respond in the same way. Such differential patterns of plasticity may be important in community dynamics, as they
affect the interactions among species. 相似文献
48.
Sybryn L. Maes Michael P. Perring Margot Vanhellemont Leen Depauw Jan Van den Bulcke Guntis Brmelis Jrg Brunet Guillaume Decocq Jan den Ouden Werner Hrdtle Radim Hdl Thilo Heinken Steffi Heinrichs Bogdan Jaroszewicz Martin Kopecký Frantiek Mli Monika Wulf Kris Verheyen 《Global Change Biology》2019,25(1):201-217
Forecasting the growth of tree species to future environmental changes requires a better understanding of its determinants. Tree growth is known to respond to global‐change drivers such as climate change or atmospheric deposition, as well as to local land‐use drivers such as forest management. Yet, large geographical scale studies examining interactive growth responses to multiple global‐change drivers are relatively scarce and rarely consider management effects. Here, we assessed the interactive effects of three global‐change drivers (temperature, precipitation and nitrogen deposition) on individual tree growth of three study species (Quercus robur/petraea, Fagus sylvatica and Fraxinus excelsior). We sampled trees along spatial environmental gradients across Europe and accounted for the effects of management for Quercus. We collected increment cores from 267 trees distributed over 151 plots in 19 forest regions and characterized their neighbouring environment to take into account potentially confounding factors such as tree size, competition, soil conditions and elevation. We demonstrate that growth responds interactively to global‐change drivers, with species‐specific sensitivities to the combined factors. Simultaneously high levels of precipitation and deposition benefited Fraxinus, but negatively affected Quercus’ growth, highlighting species‐specific interactive tree growth responses to combined drivers. For Fagus, a stronger growth response to higher temperatures was found when precipitation was also higher, illustrating the potential negative effects of drought stress under warming for this species. Furthermore, we show that past forest management can modulate the effects of changing temperatures on Quercus’ growth; individuals in plots with a coppicing history showed stronger growth responses to higher temperatures. Overall, our findings highlight how tree growth can be interactively determined by global‐change drivers, and how these growth responses might be modulated by past forest management. By showing future growth changes for scenarios of environmental change, we stress the importance of considering multiple drivers, including past management and their interactions, when predicting tree growth. 相似文献
49.
Verstraeten VL Renes J Ramaekers FC Kamps M Kuijpers HJ Verheyen F Wabitsch M Steijlen PM van Steensel MA Broers JL 《Histochemistry and cell biology》2011,135(3):251-261
A thorough understanding of fat cell biology is necessary to counter the epidemic of obesity. Although molecular pathways
governing adipogenesis are well delineated, the structure of the nuclear lamina and nuclear-cytoskeleton junction in this
process are not. The identification of the ‘linker of nucleus and cytoskeleton’ (LINC) complex made us consider a role for
the nuclear lamina in adipose conversion. We herein focused on the structure of the nuclear lamina and its coupling to the
vimentin network, which forms a cage-like structure surrounding individual lipid droplets in mature adipocytes. Analysis of
a mouse and human model system for fat cell differentiation showed fragmentation of the nuclear lamina and subsequent loss
of lamins A, C, B1 and emerin at the nuclear rim, which coincides with reorganization of the nesprin-3/plectin/vimentin complex
into a network lining lipid droplets. Upon 18 days of fat cell differentiation, the fraction of adipocytes expressing lamins
A, C and B1 at the nuclear rim increased, though overall lamin A/C protein levels were low. Lamin B2 remained at the nuclear
rim throughout fat cell differentiation. Light and electron microscopy of a subcutaneous adipose tissue specimen showed striking
indentations of the nucleus by lipid droplets, suggestive for an increased plasticity of the nucleus due to profound reorganization
of the cellular infrastructure. This dynamic reorganization of the nuclear lamina in adipogenesis is an important finding
that may open up new venues for research in and treatment of obesity and nuclear lamina-associated lipodystrophy. 相似文献
50.
Anne McHugh Paulette Bierzychudek Christina Greever Tessa Marzulla Richard Van Buskirk Greta Binford 《Journal of Insect Conservation》2013,17(6):1237-1253
The genetic structure of lineages can provide important information for delineating “evolutionarily significant units” (ESUs) for conservation, and for planning actions to protect and restore taxa threatened with extinction. Speyeria zerene hippolyta, the Oregon silverspot butterfly, is a U.S.A. federally threatened subspecies that is the focus of considerable conservation effort, but whose evolutionary relationships with other Speyeria taxa are not well-understood. We conducted a genetic analysis of nine Speyeria species and 25 subspecies from western U.S.A., using both mitochondrial and nuclear markers. Our goal was to determine whether such data supported (a) S. z. hippolyta’s designation as an ESU, and (b) the current morphologically-based taxonomy of Speyeria spp. Our data for S. z. hippolyta were equivocal; while nuclear markers resolved all these individuals into a single clade, mtDNA data suggested the existence of two clades. Aside from S. cybele, which was consistently supported as monophyletic, our data provided little support for most of the species currently recognized for western U.S. Speyeria, including S. zerene, and even less for the many subspecies designations. These genetic findings stand in contrast to the morphological differences recognized by experts, and suggest a relatively recent origin for many of these taxa. Two of 66 individuals screened for Wolbachia infection tested positive for this symbiont. Our results provide no persuasive evidence that S. z. hippolyta should lose its status as an ESU, but they have important implications for ongoing management actions such as population augmentation. 相似文献