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991.
Effects of defoliation intensity on soil food-web properties in an experimental grassland community 总被引:1,自引:0,他引:1
Juha Mikola Gregor W. Yeates Gary M. Barker David A. Wardle Karen I. Bonner 《Oikos》2001,92(2):333-343
We established a greenhouse experiment based on replicated mini‐ecosystems to evaluate the effects of defoliation intensity on soil food‐web properties in grasslands. Plant communities, composed of white clover (Trifolium repens), perennial ryegrass (Lolium perenne) and plantain (Plantago lanceolata) with well‐established root and shoot systems, were subjected to five defoliation intensity treatments: no trimming (defoliation intensity 0, or DI 0), and trimming of all plant material to 35 cm (DI 1), 25 cm (DI 2), 15 cm (DI 3) and 10 cm (DI 4) above soil surface every second week for 14 weeks. Intensification of defoliation reduced shoot production and standing shoot and root mass of plant communities but increased their root to shoot ratio. Soil microbial activity and biomass decreased with intensification of defoliation. Concentrations of NO3–N in soil steadily increased with intensifying defoliation, whereas NH4–N concentrations did not vary between treatments. Numbers of microbi‐detritivorous enchytraeids, bacterial‐feeding rotifers and bacterial‐feeding nematodes steadily increased with intensifying defoliation, while the abundance of fungal‐feeding nematodes was significantly enhanced only in DI 3 and DI 4 relative to DI 0. The abundance of herbivorous nematodes per unit soil mass was lower in DI 3 and DI 4 than in DI 0, DI 1 and DI 2, but when calculated per unit root mass, their abundance tended to increase with defoliation intensity. The abundance of omnivorous and predatory nematodes appeared to be highest in the most intensely defoliated systems. The ratio of abundance of fungal‐feeding nematodes to that of bacterial‐feeding nematodes was not significantly affected by defoliation intensity. The results infer that defoliation intensity may significantly alter the structure of soil food webs in grasslands, and that defoliation per se is able to induce patterns observed in grazing studies in the field. The results did not support hypotheses that defoliation per se would cause a shift between the bacterial‐based and fungal‐based energy channels in the decomposer food web, or that herbivore and detritivore densities in soil would be highest under intermediate defoliation. Furthermore, our data for microbes and microbial feeders implies that the effects of defoliation intensity on soil food‐web structure may depend on the duration of defoliation and are therefore likely to be dynamic rather than constant in nature. 相似文献
992.
Abiotic stresses increasingly threaten existing ecological and agricultural systems across the globe. Plant roots perceive these stresses in the soil and adapt their architecture accordingly. This review provides insights into recent discoveries showing the importance of root system architecture (RSA) and plasticity for the survival and development of plants under heat, cold, drought, salt, and flooding stress. In addition, we review the molecular regulation and hormonal pathways involved in controlling RSA plasticity, main root growth, branching and lateral root growth, root hair development, and formation of adventitious roots. Several stresses affect root anatomy by causing aerenchyma formation, lignin and suberin deposition, and Casparian strip modulation. Roots can also actively grow toward favorable soil conditions and avoid environments detrimental to their development. Recent advances in understanding the cellular mechanisms behind these different root tropisms are discussed. Understanding root plasticity will be instrumental for the development of crops that are resilient in the face of abiotic stress.Recent discoveries show the importance of root system architecture plasticity for the survival and growth of plants under several abiotic stresses. 相似文献
993.
Naomi D. de Bruijne Kedir Urgesa Abraham Aseffa Kidist Bobosha Anne Schoenmakers Robin van Wijk Thomas Hambridge Mitzi M. Waltz Christa Kasang Liesbeth Mieras 《PLoS neglected tropical diseases》2022,16(1)
BackgroundDelay in case detection is a risk factor for developing leprosy-related impairments, leading to disability and stigma. The objective of this study was to develop a questionnaire to determine the leprosy case detection delay, defined as the period between the first signs of the disease and the moment of diagnosis, calculated in total number of months. The instrument was developed as part of the PEP4LEP project, a large-scale intervention study which determines the most effective way to implement integrated skin screening and leprosy post-exposure prophylaxis with a single-dose of rifampicin (SDR-PEP) administration in Ethiopia, Mozambique and Tanzania.Methodology/Principal findingsA literature review was conducted and leprosy experts were consulted. The first draft of the questionnaire was developed in Ethiopia by exploring conceptual understanding, item relevance and operational suitability. Then, the first draft of the tool was piloted in Ethiopia, Mozambique and Tanzania. The outcome is a questionnaire comprising nine questions to determine the case detection delay and two annexes for ease of administration: a local calendar to translate the patient’s indication of time to number of months and a set of pictures of the signs of leprosy. In addition, a body map was included to locate the signs. A ‘Question-by-Question Guide’ was added to the package, to provide support in the administration of the questionnaire. The materials will be made available in English, Oromiffa (Afaan Oromo), Portuguese and Swahili via https://www.infolep.org.Conclusions/SignificanceIt was concluded that the developed case detection delay questionnaire can be administered quickly and easily by health workers, while not inconveniencing the patient. The instrument has promising potential for use in future leprosy research. It is recommended that the tool is further validated, also in other regions or countries, to ensure cultural validity and to examine psychometric properties like test-retest reliability and interrater reliability. 相似文献
994.
Sebastian Persson Niek Welkenhuysen Sviatlana Shashkova Samuel Wiqvist Patrick Reith Gregor W. Schmidt Umberto Picchini Marija Cvijovic 《PLoS computational biology》2022,18(5)
Understanding the inherited nature of how biological processes dynamically change over time and exhibit intra- and inter-individual variability, due to the different responses to environmental stimuli and when interacting with other processes, has been a major focus of systems biology. The rise of single-cell fluorescent microscopy has enabled the study of those phenomena. The analysis of single-cell data with mechanistic models offers an invaluable tool to describe dynamic cellular processes and to rationalise cell-to-cell variability within the population. However, extracting mechanistic information from single-cell data has proven difficult. This requires statistical methods to infer unknown model parameters from dynamic, multi-individual data accounting for heterogeneity caused by both intrinsic (e.g. variations in chemical reactions) and extrinsic (e.g. variability in protein concentrations) noise. Although several inference methods exist, the availability of efficient, general and accessible methods that facilitate modelling of single-cell data, remains lacking. Here we present a scalable and flexible framework for Bayesian inference in state-space mixed-effects single-cell models with stochastic dynamic. Our approach infers model parameters when intrinsic noise is modelled by either exact or approximate stochastic simulators, and when extrinsic noise is modelled by either time-varying, or time-constant parameters that vary between cells. We demonstrate the relevance of our approach by studying how cell-to-cell variation in carbon source utilisation affects heterogeneity in the budding yeast Saccharomyces cerevisiae SNF1 nutrient sensing pathway. We identify hexokinase activity as a source of extrinsic noise and deduce that sugar availability dictates cell-to-cell variability. 相似文献
995.
The negative chemical ionization (NCI) mass spectra of a series of bis(2,2,6,6-tetramethylheptane-3,5-dionato)metal(II) complexes [metal = Co, Ni, Cu, Zn] obtained using the halogenated reagent gases, NF3, CF2Cl2 and CF3Br, are presented. Thermalized electrons are captured by the Co, Ni and Cu complexes to produce molecular ions in which the oxidation state of the metal has been reduced. Lewis acid-base addition of halide ions to the metal to produce adduct ions occurs with each of the metal complexes. Reactions between radicals and the complexes also occur to produce adduct molecules which may be ionized by thermalized electrons or halide ions in the same manner as the parent complexes. Halogen radicals reacts by ligand displacement and homolytic substitution of the ligand while halogenated methyl radicals undergo oxidative-addition to the metal. Clustering reactions to produce ions containing two metal atoms also occur. 相似文献
996.
997.
By learning to discriminate among visual stimuli, human observers can become experts at specific visual tasks. The same is true for Rhesus monkeys, the major animal model of human visual perception. Here, we systematically compare how humans and monkeys solve a simple visual task. We trained humans and monkeys to discriminate between the members of small natural-image sets. We employed the "Bubbles" procedure to determine the stimulus features used by the observers. On average, monkeys used image features drawn from a diagnostic region covering about 7% +/- 2% of the images. Humans were able to use image features drawn from a much larger diagnostic region covering on average 51% +/- 4% of the images. Similarly for the two species, however, about 2% of the image needed to be visible within the diagnostic region on any individual trial for correct performance. We characterize the low-level image properties of the diagnostic regions and discuss individual differences among the monkeys. Our results reveal that monkeys base their behavior on confined image patches and essentially ignore a large fraction of the visual input, whereas humans are able to gather visual information with greater flexibility from large image regions. 相似文献
998.
The neuropeptides oxytocin (OXT) and arginine vasopressin (AVP) are evolutionarily highly conserved mediators in the regulation of complex social cognition and behaviour. Recent studies have investigated the effects of OXT and AVP on human social interaction, the genetic mechanisms of inter-individual variation in social neuropeptide signalling and the actions of OXT and AVP in the human brain as revealed by neuroimaging. These data have advanced our understanding of the mechanisms by which these neuropeptides contribute to human social behaviour. OXT and AVP are emerging as targets for novel treatment approaches--particularly in synergistic combination with psychotherapy--for mental disorders characterized by social dysfunction, such as autism, social anxiety disorder, borderline personality disorder and schizophrenia. 相似文献
999.
Antibody to prekeratin : Decoration of tonofilament-like arrays in various cells of epithelial character 总被引:66,自引:0,他引:66
Werner W. Franke Klaus Weber Mary Osborn Erika Schmid Christa Freudenstein 《Experimental cell research》1978,116(2):429-445
Certain epithelial cells in culture such as the established rat kangaroo cell lines PtK1 and PtK2, mammary gland epithelial cells from cow udder, and human kidney epithelial cells are characterized by a system of wavy, branching and aggregating arrays of filaments of diameters 6–11 nm which are often desmosome-associated. Antibodies raised in guinea pigs against purified bovine prekeratin specifically decorate this system of tonofilament bundles in indirect immunofluorescence microscopy. In agreement with these results we show that preparations of these filaments isolated from such epithelial cells contain some proteins similar in polypeptide size and behaviour to components of bovine hoof prekeratin and to bovine muzzle tonofilaments. We therefore conclude that several epithelial cells which are capable of continuous division in culture continuously produce large, balanced amounts of prekeratin-like material which is assembled in tonofilament-like structures. 相似文献
1000.
Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent 总被引:52,自引:0,他引:52
The apoprotein of yeast cytochrome c1 is made outside the mitochondria as a larger precursor which is then processed in at least two steps. In the first step, it is transported across both mitochondrial membranes and converted by a matrix-localized protease to an intermediate form whose molecular weight is between that of the precursor and the mature form. The intermediate form is bound to the outer face of the inner membrane. This first step requires an energized mitochondrial inner membrane, but no heme. In the second step, the intermediate form is converted to the mature cytochrome. This second step requires heme; it is blocked in a heme-deficient mutant or in wild type cells treated with an inhibitor of heme synthesis. Import of cytochrome c1 into mitochondria thus proceeds via two distinct heme-free precursors and at least two maturation steps, one of them dependent on heme. 相似文献