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Myogenin, MyoD, and myosin expression after pharmacologically and surgically induced hypertrophy 总被引:2,自引:0,他引:2
The relationshipbetween myogenin or MyoD expression and hypertrophy of the rat soleusproduced either by clenbuterol and 3,3',5-triiodo-L-thyronine(CT) treatment or by surgical overload was examined. Mature female ratswere subjected to surgical overload of the right soleus with the leftsoleus serving as a control. Another group received the same surgicaltreatment but were administered CT. Soleus muscles were harvested 4 wkafter surgical overload and weighed. Myosin heavy chain isoforms wereseparated by using polyacrylamide gel electrophoresis while myogeninand MyoD expression were evaluated by Northern analysis.CT and functional overload increased soleus muscle weight. CT treatmentinduced the appearance of the fast type IIX myosin heavy chain isoform,depressed myogenin expression, and induced MyoD expression. However,functional overload did not alter myogenin or MyoD expression inCT-treated or non-CT-treated rats. Thus pharmacologically andsurgically induced hypertrophy have differing effects on myogenin andMyoD expression, because their levels were associated with changes inmyosin heavy chain composition (especially type IIX) rather thanchanges in muscle mass. 相似文献
114.
Ierecê ML Rosa Rômulo RN Alves Kallyne M Bonifácio José S Mourão Frederico M Osório Tacyana PR Oliveira Mara C Nottingham 《Journal of ethnobiology and ethnomedicine》2005,1(1):1-15
From a conservationist perspective, seahorses are threatened fishes. Concomitantly, from a socioeconomic perspective, they represent a source of income to many fishing communities in developing countries. An integration between these two views requires, among other things, the recognition that seahorse fishers have knowledge and abilities that can assist the implementation of conservation strategies and of management plans for seahorses and their habitats. This paper documents the knowledge held by Brazilian fishers on the biology and ecology of the longsnout seahorse Hippocampus reidi. Its aims were to explore collaborative approaches to seahorse conservation and management in Brazil; to assess fishers' perception of seahorse biology and ecology, in the context evaluating potential management options; to increase fishers' involvement with seahorse conservation in Brazil. Data were obtained through questionnaires and interviews made during field surveys conducted in fishing villages located in the States of Piauí, Ceará, Paraíba, Maranhão, Pernambuco and Pará. We consider the following aspects as positive for the conservation of seahorses and their habitats in Brazil: fishers were willing to dialogue with researchers; although captures and/or trade of brooding seahorses occurred, most interviewees recognized the importance of reproduction to the maintenance of seahorses in the wild (and therefore of their source of income), and expressed concern over population declines; fishers associated the presence of a ventral pouch with reproduction in seahorses (regardless of them knowing which sex bears the pouch), and this may facilitate the construction of collaborative management options designed to eliminate captures of brooding specimens; fishers recognized microhabitats of importance to the maintenance of seahorse wild populations; fishers who kept seahorses in captivity tended to recognize the condtions as poor, and as being a cause of seahorse mortality. 相似文献
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Andrew ML Lever 《Retrovirology》2006,3(1):1-4
The 2006 M Jeang Retrovirology Prize for HIV research has been awarded to Dr Joe Sodroski 相似文献
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α2-Tropomyosin crystals can be distinguished from those obtained from unfractionated tropomyosin by phase contrast microscopy and by their electron microscopic appearance after thin sectioning and negative staining. The overlapping of two conventional kite-shaped tropomyosin nets gives projections that are similar to those obtained from the new α2-tropomyosin crystals. Both symmetrical and unsymmetrical overlap patterns were observed. These crystals may prove to be useful for X-ray diffraction studies because of their presumably higher protein densities. 相似文献