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221.
Grazia Fazio Carles Gaston‐Massuet Laura Rachele Bettini Federica Graziola Valeria Scagliotti Anna Cereda Luca Ferrari Mara Mazzola Gianni Cazzaniga Antonio Giordano Franco Cotelli Gianfranco Bellipanni Andrea Biondi Angelo Selicorni Anna Pistocchi Valentina Massa 《Journal of cellular physiology》2016,231(3):613-622
222.
Valeria De Arcangelis Georgios Strimpakos Francesca Gabanella Nicoletta Corbi Siro Luvisetto Armando Magrelli Annalisa Onori Claudio Passananti Cinzia Pisani Sophie Rome Cinzia Severini Fabio Naro Elisabetta Mattei Maria Grazia Di Certo Lucia Monaco 《Journal of cellular physiology》2016,231(1):224-232
223.
Grazia Luisi Adriano Mollica Simone Carradori Alessia Lenoci Anastasia De Luca 《Journal of enzyme inhibition and medicinal chemistry》2016,31(6):924-930
Context: The inhibition of glutathione S-transferase P1-1 (GSTP1-1) is a sound strategy to overcome drug resistance in oncology practice.Objective: The nitrobenzoxadiazolyl (NBD) S-conjugate of glutathione and the corresponding γ-oxa-glutamyl isostere (compounds 1 and 5, respectively) have been disclosed as GST inhibitors. The rationale of their design is discussed in juxtaposition to non-peptide NBD thioethers.Materials and methods: Synthesis of derivatives 1 and 5 and in vitro evaluation on human GSTP1-1 and M2-2 are reported.Results: Conjugates 1 and 5 were found to be low micromolar inhibitors of both isoforms. Furthermore, they display a threefold reduction in selectivity for GSTM2-2 over the P1-1 isozyme in comparison with the potent non-peptide inhibitor nitrobenzoxadiazolyl-thiohexanol (NBDHEX).Discussion and conclusions: Spectroscopic data are congruent with the formation of a stable sigma-complex between GSH and the inhibitors in the protein active site. Conjugate 5 is suitable for in vivo modulation of GST activity in cancer treatment. 相似文献
224.
225.
Grazia Fernanda Spitoni Giorgio Pireddu Gaspare Galati Valentina Sulpizio Stefano Paolucci Luigi Pizzamiglio 《PloS one》2016,11(3)
Central post-stroke pain is a neuropathic syndrome characterized by intolerable contralesional pain and, in rare cases, somatic delusions. To date, there is limited evidence for the effective treatments of this disease. Here we used caloric vestibular stimulation to reduce pain and somatoparaphrenia in a 57-year-old woman suffering from central post-stroke pain. Resting-state functional magnetic resonance imaging was used to assess the neurological effects of this treatment. Following vestibular stimulation we observed impressive improvements in motor skills, pain, and somatic delusions. In the functional connectivity study before the vestibular stimulation, we observed differences in the patient’s left thalamus functional connectivity, with respect to the thalamus connectivity of a control group (N = 20), in the bilateral cingulate cortex and left insula. After the caloric stimulation, the left thalamus functional connectivity with these regions, which are known to be involved in the cortical response to pain, disappeared as in the control group. The beneficial use of vestibular stimulation in the reduction of pain and somatic delusion in a CPSP patient is now documented by behavioral and imaging data. This evidence can be applied to theoretical models of pain and body delusions. 相似文献
226.
Effects of cadmium on lymphocyte activation 总被引:2,自引:0,他引:2
M Grazia Cifone E Alesse A Procopio R Paolini S Morrone R Di Eugenio G Santoni A Santoni 《Biochimica et biophysica acta》1989,1011(1):25-32
The effects of cadmium (Cd) on phytohemoagglutinin or phorbol myristate acetate-induced lymphocyte activation were investigated and a dose-dependent inhibition of cell proliferation was found. Kinetic studies revealed that the Cd-sensitive step is an early event of T cell stimulation. Failure of IL2 secretion and reduction of IL2 receptor expression in the Cd-treated cells are also reported. Regardless of which mechanism is responsible for Cd effects, our studies show that the inhibition of lymphocyte activation is associated with reduced [3H]phorbol dibutyrate binding to Ca2+-phospholipid-dependent protein kinase and altered breakdown of phosphatidylinositols. Thus, Cd interferes with two biochemical events which play a critical role in lymphocyte signal transduction and activation. 相似文献
227.
Gianni Dehò Daniela Ghisotti Pietro Aland Sandro Zangrossi Maria Grazia Borrello Gianpiero Sironi 《Journal of molecular biology》1984,178(2):191-207
The satellite bacteriophage P4, in the presence of a helper phage, can enter either the lytic or the lysogenic cycle. In the absence of the helper, P4 can integrate in the bacterial chromosome. In addition, the partially immunity-insensitive mutant P4 vir1 maintained as a plasmid.We have found that in the absence of the helper, P4 wt also can be maintained as a plasmid, and that both P4 wt and P4 vir1 have two options for their intracellular propagation: a repressed-integrated or a derepressed-high copy number plasmid mode of maintenance. In the repressed mode, the P4 wt genome is only found integrated into the bacterial chromosome, while the P4 vir1 is found also as a low copy number plasmid coexisting with the integrated P4 vir1 genome. The clones carrying P4 in the derepressed-high copy number plasmid state are obtained at low frequency (0.3%) upon infection with P4 wt, while the vir1 mutation increases this frequency about 300-fold. Such clones can be distinguished easily because of their typical colony morphology (rosettes), due to the presence of filamentous cells. Filamentation of the bacterial host suggests that the presence of derepressed P4 genomes in high copy number interferes with the normal cell division mechanism.The derepressed clones are rather stable, but may revert spontaneously to the repressed state. Spontaneous transition from the repressed to the derepressed state was not observed; however, it can be induced by P2 or P4 vir1 superinfection of P4 wt and P4 vir1 lysogens or by growing the P4 vir1 lysogens up to the late exponential phase.The ability of P4 to choose either of two stable states and the potential to shift between these two modes of propagation indicate that the syntesis of the immunity repressor is regulated. 相似文献
228.
Grazia Marino 《Plant Growth Regulation》1988,7(4):237-247
The effect of paclobutrazol on in vitro rooting and growth of sour cherry (Prunus cerasus) rootstock CAB 11E clone, of S 749 × S 1490 (Prunus persica × Prunus kansuensis) hybrid rootstock, and of pear (Pyrus communis), cv. Abbé Fetel is reported.PP333 increased rooting of S 749 × S 1490 and of Abbé Fetel, particularly at a concentration of 0.5 mg/l (a.i.); moreover, it induced a rooting percentage as high as auxin in the former and hastened rooting of the latter. By contrast, paclobutrazol did not affect root production of 11 E.PP333-treated plants had shorter and thicker roots than controls but similar survival rates during acclimatization. Otherwise they grew less than controls during the first part of the acclimatization phase.Abbreviations used in text and tables BA =
6-benzyladenine
- IBA =
indole-3-butyric acid
- PP333 =
paclobutrazol = (2RS,3RS)-1-(-4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pentan-3-ol
Part of the results referring to S 749 × S 1490 (P. persica × P. kansuensis) rootstock were presented at the meeting on Controllo della fruttificazione delle piante da frutto, Bologna, Italy, June 1986, and were published in the Riv. Ortoflorofrutt. It. 70 (6)(1986). This research was funded in part by the Italian Ministry of Education (M.P.I. 60%). 相似文献
229.
Daniela Rossi Mariangela Urbano Alice Pedrali Massimo Serra Daniele Zampieri Maria Grazia Mamolo Christian Laggner Caterina Zanette Chiara Florio Dirk Schepmann Bernard Wuensch Ornella Azzolina Simona Collina 《Bioorganic & medicinal chemistry》2010,18(3):1204-1212
In order to investigate the molecular features involved in sigma receptors (σ-Rs) binding, new compounds based on arylalkylaminoalcoholic, arylalkenyl- and arylalkylaminic scaffolds were synthesized and their affinity towards σ1- and σ2-Rs subtypes was evaluated. The most promising compounds were also screened for their affinity at μ-opioid, δ-opioid and κ-opioid receptors. Biological results are herein presented and discussed. 相似文献