全文获取类型
收费全文 | 161篇 |
免费 | 26篇 |
专业分类
187篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 15篇 |
2011年 | 19篇 |
2010年 | 10篇 |
2009年 | 3篇 |
2008年 | 6篇 |
2007年 | 10篇 |
2006年 | 14篇 |
2005年 | 8篇 |
2004年 | 9篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1981年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 2篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1961年 | 1篇 |
1960年 | 2篇 |
1959年 | 1篇 |
1958年 | 1篇 |
1956年 | 1篇 |
1955年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有187条查询结果,搜索用时 0 毫秒
181.
182.
Elina Payet Pascal Dimitrov‐Raytchev Bastien Chatelet Laure Guy Stephane Grass Jerome Lacour Jean‐Pierre Dutasta Alexandre Martinez 《Chirality》2012,24(12):1077-1081
The hemicryptophane racemate (±)‐ M-1 , P-1 was optically resolved by semipreparative HPLC on Chiralpak IC column. The absolute configuration of each isolated enantiomer was established from the analysis of their electronic circular dichroism spectra. Enantiodifferentiation of the chiral cationic cage (±)‐ M-1 , P-1 was evidenced in solution using Δ‐TRISPHAT as chiral solvating agent, and the diastereomeric associations were observed in 1H and 31P NMR spectra. Chirality 24:1077–1081, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
183.
184.
185.
Jakub Jez Alexandra Castilho Josephine Grass Karola Vorauer-Uhl Thomas Sterovsky Friedrich Altmann Prof. Herta Steinkellner 《Biotechnology journal》2013,8(3):371-382
Recombinant human erythropoietin (rhEPO), a glycohormone, is one of the leading biopharmaceutical products. The production of rhEPO is currently restricted to mammalian cell expression systems because of rhEPO's highly complex glycosylation pattern, which is a major determinant for drug-efficacy. Here we evaluate the ability of plants to produce different glycoforms of rhEPO. cDNA constructs were delivered to Nicotiana benthamiana (N. benthamiana) and transiently expressed by a viral based expression system. Expression levels up to 85 mg rhEPO/kg fresh leaf material were achieved. Moreover, co-expression of rhEPO with six mammalian genes required for in planta protein sialylation resulted in the synthesis of rhEPO decorated mainly with bisialylated N-glycans (NaNa), the most abundant glycoform of circulating hEPO in patients with anemia. A newly established peptide tag (ELDKWA) fused to hEPO was particularly well-suited for purification of the recombinant hormone based on immunoaffinity. Subsequent lectin chromatography allowed enrichment of exclusively sialylated rhEPO. All plant-derived glycoforms exhibited high biological activity as determined by a cell-based receptor-binding assay. The generation of rhEPO carrying largely homogeneous glycosylation profiles (GnGnXF, GnGn, and NaNa) will facilitate further investigation of functionalities with potential implications for medical applications. 相似文献
186.
187.
Grass F. Bichler M. Dorner J. Holzner H. Ritschel A. Ramadan A. Westphal G. P. Gwozdz R. 《Biological trace element research》1994,43(1):33-46
The application of short-lived nuclides, especially in connection with the6LiD-converter, in biological and environmental samples is demonstrated on I and Br determination in human urine, on I in pet food, and on the analysis of all the halogens in volcanic gases in a single activation. Trace element determination in lichens indicates polluted and unpolluted areas. The use of the 74-s38mCl enables the rapid screening of great number of samples.
相似文献