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Under the same solution conditions, the apparent weight average sedimentation coefficient, swa, and some quantities obtained from it can be combined with the equilibrium constant or constants, Ki, and the monomer concentration, cI, obtained from sedimentation equilibrium, light scattering or osmotic pressure experiments on the same self-associating solute, so that the individual sedimentation coefficients, si, of the self-associating species, and also the hydrodynamic concentration dependence parameter,g or , can be evaluated. Using two different models for the hydrodynamic concentration parameter, four different methods are presented for the evaluation of the si's. Methods for evaluating g or , once the si's are known, are presented. A method for obtaining the number average sedimentation coefficient, sN, and its application to self-associations is presented. Methods are shown for the evaluation of Z average properties, xzc, as well as number average properties,xNc, of a self-associating solute from its weight average properties, xwc. 相似文献
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S. Stephenson A. A. Chariton M. P. Holley M. O’Sullivan M. R. Gillings G. C. Hose 《Geomicrobiology journal》2013,30(7):623-634
Groundwater biota are particularly sensitive to environmental perturbations such as groundwater contamination. The diversity of prokaryotic and eukaryotic biota has been examined along a gradient of chlorinated hydrocarbon (CHC) contamination in the Botany Sands, an urban coastal sand-bed aquifer (Sydney, Australia). Molecular techniques were used to analyze the richness and composition of prokaryote and eukaryote assemblages using 16S and 18S rDNA, respectively. Taxon richness did not change significantly along the gradient for either prokaryotes or eukaryotes; however, significant shifts in assemblage composition were evident for both groups. Assemblage changes were most strongly correlated with concentrations of the major CHC, cis-1,2-dichloroethene, but the concentrations of a number of the contaminants were also correlated, making it difficult to infer if effects were due to any particular contaminant. The presence of cis-1,2-dichloroethene and other secondary ethenes suggests in situ breakdown of the primary CHCs via natural attenuation. The current focus of management of the Botany aquifer is to stop the contaminant plume reaching the adjoining estuary. This approach is clearly justified given the changes evident in the microbial assemblages in the groundwater, which are a likely consequence of the contamination. 相似文献
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Summary A complex, higly sulphated glucoxylomannogalactan has been extracted in a yield of about 1% dry weight fromC. simpliciuscula. This polysaccharide is similar in composition to sulphated polysaccharides previously isolated from otherCaulerpa species (Mackie andPercival, 1961). The most likely location of this compound in the unwounded cell is in the vacuole. This polysaccharide appears as the major component in wound plugs, forming a viscoelastic barrier between the protoplasm and the external environment. The properties of the sulphated polysaccharide were studied in an effort to understand the physiology and mechanism of wound plug formation. 相似文献