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101.
F. Darío Cuello-Carrión Niubys Cayado-Gutiérrez Anthony L. Natoli Christina Restall Robin L. Anderson Silvina Nadin Daiana Alvarez-Olmedo Gisela N. Castro Francisco E. Gago Mariel A. Fanelli Daniel R. Ciocca 《Cell stress & chaperones》2013,18(5):559-567
In a recent study, we have shown that in mammary tumors from mice lacking the Cav-1 gene, there are alterations in specific heat shock proteins as well as in tumor development. With this in mind, we have now investigated other proteins in the same mammary mouse tumor model (Her-2/neu expressing mammary tumors from Cav-1 wild type and Cav-1 null mice), to further comprehend the complex tumor-stroma mechanisms involved in regulating stress responses during tumor development. In this tumor model the cancer cells always lacked of Cav-1, so the KO influenced the Cav-1 in the stroma. By immunohistochemistry, we have found a striking co-expression of β-catenin and Her-2/neu in the tumor cells. The absence of Cav-1 in the tumor stroma had no effect on expression or localization of β-catenin and Her-2/neu. Both proteins appeared co-localized at the cell surface during tumor development and progression. Since Her-2/neu activation induces MTA1, we next evaluated MTA1 in the mouse tumors. Although this protein was found in numerous nuclei, the absence of Cav-1 did not alter its expression level. In contrast, significantly more PTEN protein was noted in the tumors lacking Cav-1 in the stroma, with the protein localized mainly in the nuclei. P-Akt levels were relatively low in tumors from both Cav-1 WT and Cav-1 KO mice. There was also an increase in nuclear NHERF1 expression levels in the tumors arising from Cav-1 KO mice. The data obtained in the MMTV-neu model are consistent with a role for Cav-1 in adjacent breast cancer stromal cells in modulating the expression and localization of important proteins implicated in tumor cell behavior. 相似文献
102.
The soil seed bank is a dynamic biotic component of plant communities that represents the population’s memory in relation
to selective events. Few studies have investigated the natural stock of germinable seeds in the gallery forests to evaluate
their regeneration potential, although they are target of anthropogenic action. Thus, seasonal, horizontal, and vertical qualitative
and quantitative variations of the seed bank of a gallery forest in the Brazilian Cerrado were studied to test the influence
of the climatic seasonality, the influence of the physical structure, and depth of the soil in different microhabitats of
the forest in this natural seed store. It was also compared the richness and abundance of species in the germinable seed bank
with the above-ground vegetation. Three hundred and seventy-five soil samples were collected at the beginning (April 1998
and 1999) and at the end of the dry season (September 1998). These samples were collected in three microhabitats distributed
transversally in relation to drainage line of a large stream, at five depths. The density of germinable seeds decreases with
depth, and is similar among microhabitats and seasons. In 24,690 cm3 of soil and 4.05 m2 of litter, 1390 seedlings emerged, being 743 dicotyledons and 647 monocotyledons. From 761 survivors, 263 were Cyperaceae,
206 Melastomataceae, 153 Poaceae, and 82 Onagraceae, the most abundant families. This study suggests that the diversity of
the germinable soil seed bank is lower than that of the above-ground vegetation of the forest, and that the soil seed bank
is not the principal regeneration form of this environment. 相似文献
103.
Fibronectin From Oviductal Cells Fluctuates During the Estrous Cycle and Contributes to Sperm–Oviduct Interaction in Cattle
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104.
Marinei Cristina Pereira Ribeiro Nilda Berenice de Vargas Barbosa Tielle Moraes de Almeida Lutiane Mozzaquatro Parcianello Juliano Perottoni Daiana Silva de vila Joo Batista Teixeira Rocha 《Cell biochemistry and function》2009,27(7):473-478
This study evaluated the effect of possible synergic interaction between high fat diet (HF) and hydrochlorothiazide (HCTZ) on biochemical parameters of oxidative stress in brain. Rats were fed for 16 weeks with a control diet or with an HF, both supplemented with different doses of HCTZ (0.4, 1.0, and 4.0 g kg−1 of diet). HF associated with HCTZ caused a significant increase in lipid peroxidation and blood glucose levels. In addition, HF ingestion was associated with an increase in cerebral lipid peroxidation, vitamin C and non‐protein thiol groups (NPSH) levels. There was an increase in vitamin C as well as NPSH levels in HCTZ (1.0 and 4.0 g kg−1 of diet) and HF plus HCTZ groups. Na+–K+‐ATPase activity of HCTZ (4.0 g kg−1 of diet) and HCTZ plus HF‐fed animals was significantly inhibited. Our data indicate that chronic intake of a high dose of HCTZ (4 g kg−1 of diet) or HF change biochemical indexes of oxidative stress in rat brain. Furthermore, high‐fat diets consumption and HCTZ treatment have interactive effects on brain, showing that a long‐term intake of high‐fat diets can aggravate the toxicity of HCTZ. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
105.
Hussein Khalil Roberta Santana Daiana de Oliveira Fabiana Palma Ricardo Lustosa Max T. Eyre Ticiana Carvalho-Pereira Mitermayer G. Reis Albert I. Ko Peter J. Diggle Yeimi Alzate Lopez Mike Begon Federico Costa 《PLoS neglected tropical diseases》2021,15(3)
Residents of urban slums suffer from a high burden of zoonotic diseases due to individual, socioeconomic, and environmental factors. We conducted a cross-sectional sero-survey in four urban slums in Salvador, Brazil, to characterize how poverty and sanitation contribute to the transmission of rat-borne leptospirosis. Sero-prevalence in the 1,318 participants ranged between 10.0 and 13.3%. We found that contact with environmental sources of contamination, rather than presence of rat reservoirs, is what leads to higher risk for residents living in areas with inadequate sanitation. Further, poorer residents may be exposed away from the household, and ongoing governmental interventions were not associated with lower transmission risk. Residents at higher risk were aware of their vulnerability, and their efforts improved the physical environment near their household, but did not reduce their infection chances. This study highlights the importance of understanding the socioeconomic and environmental determinants of risk, which ought to guide intervention efforts. 相似文献
106.
Garcez Daiana Kaster Barbosa Crislaine de Oliveira Fernandes Murilo Volcan Matheus Vieira Robe Lizandra Jaqueline 《Biodiversity and Conservation》2022,31(7):1945-1967
Biodiversity and Conservation - Rivulidae represents one of the most endangered taxa in Brazil. This family encompasses species known as annual or seasonal fish because they possess unique... 相似文献
107.
Daiana Lima-Morales Ruy Jáuregui Amelia Camarinha-Silva Robert Geffers Dietmar H. Pieper Ramiro Vilchez-Vargas 《Applied and environmental microbiology》2016,82(7):2227-2237
Three types of contaminated soil from three geographically different areas were subjected to a constant supply of benzene or benzene/toluene/ethylbenzene/xylenes (BTEX) for a period of 3 months. Different from the soil from Brazil (BRA) and Switzerland (SUI), the Czech Republic (CZE) soil which was previously subjected to intensive in situ bioremediation displayed only negligible changes in community structure. BRA and SUI soil samples showed a clear succession of phylotypes. A rapid response to benzene stress was observed, whereas the response to BTEX pollution was significantly slower. After extended incubation, actinobacterial phylotypes increased in relative abundance, indicating their superior fitness to pollution stress. Commonalities but also differences in the phylotypes were observed. Catabolic gene surveys confirmed the enrichment of actinobacteria by identifying the increase of actinobacterial genes involved in the degradation of pollutants. Proteobacterial phylotypes increased in relative abundance in SUI microcosms after short-term stress with benzene, and catabolic gene surveys indicated enriched metabolic routes. Interestingly, CZE soil, despite staying constant in community structure, showed a change in the catabolic gene structure. This indicates that a highly adapted community, which had to adjust its gene pool to meet novel challenges, has been enriched. 相似文献
108.
Duarte Rafaela Cristina de Souza de Barros Graciele Milesi Silvia Vendruscolo Dias Thelma Lucia Pereira 《Hydrobiologia》2020,847(4):1107-1119
Hydrobiologia - Studies based on functional approach seek to understand the ecological roles developed by species as well as their interactions with the environment in which they are inserted. The... 相似文献
109.
Paula Daiana De Paulo Eliseu José G. Pereira Eugenio E. Oliveira Alberto Fereres Elisa Garzo 《Journal of Applied Entomology》2020,144(10):941-951
The development of herbivore insects is influenced by the quality of their host plants. Elevated CO2 alters plant metabolism, which may change the nutritional quality of the plant, modifying the life history and feeding behaviour of herbivore insects. Understanding how insect pests respond to increasing CO2 concentration is essential for predicting the impact of the pest on food security. In this study, we investigated the effects of elevated CO2 (eCO2) on the life history and feeding behaviour of the MEAM1 species of Bemisia tabaci on a Bt soybean cultivar. We found that eCO2 increased the egg to adult development time and reduced the reproductive responses (fecundity and fertility) of B. tabaci. The whitefly B. tabaci that fed on the soybean plants grown under eCO2 conditions was negatively influenced by several traits related to the host plant resistance, such as the time spent on phloem sap ingestion. Furthermore, we evaluated the changes in the C:N concentration and plant morphology of the Bt plants. The biomass (weight of leaves and stems) of the Bt soybean plants grown under eCO2 conditions was significantly increased, and the elevated C:N ratio in the phenological stage V6 (i.e. when the plants had six trifoliate leaves developed) was the most pronounced difference in the Bt soybean plants subjected to eCO2 treatment. Taken together, our results indicate that Bt plants cultivated under eCO2 inhibit B. tabaci feeding, which can reduce whitefly infestations of the soybean fields. 相似文献
110.
Carola Cañón Daiana Mir Ulyses F. J. Pardiñas Enrique P. Lessa Guillermo D'Elía 《Zoologica scripta》2014,43(5):443-454
Abrotrichini is a recently defined and diagnosed tribe of Sigmodontinae with a complex taxonomy. Abrotrichine genera, Abrothrix (including Chroeomys), Chelemys, Geoxus, Notiomys and Pearsonomys, are mostly distributed in the central and southern Andes and adjacent lowlands and show terrestrial and fossorial habits. Recent studies have evidenced some incongruence between current taxonomy and abrotrichine phylogeny, such as the polyphyly of Chelemys and paraphyly of Geoxus respect to Pearsonomys. We used DNA sequence data of six loci (one mitochondrial and five nuclear) to resolve the relationships within the tribe. Independent and combined analyses of these loci were carried out using parsimony, maximum likelihood and Bayesian inference. Estimates of divergence time of the main lineages of abrotrichines were calculated with a molecular clock using as calibration, a fossil recently found. The concatenated data set increased the resolution and defined the relationships within the tribe. Our phylogenetic analyses corroborate that Abrothrix is sister of a clade containing the long‐clawed abrotrichines. We recovered two main clades within Abrothrix that match morphologic variation and geographic distribution of its species. In addition, we corroborated the lack of monophyly of Chelemys and the lack of monophyly of Geoxus. We discuss different taxonomic scenarios to abrotrichine classification reflects the phylogenetic relationships obtained in this study. Our molecular clock estimated the Abrotrichini crown age to be around the early Pliocene (4.4 Ma) and suggest that the tribe diversified over a short period of time. 相似文献