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61.
Jose Fernández-Triana Henri Goulet Caroline Boudreault 《Biocontrol Science and Technology》2009,19(3):237-248
This study reports the Microgastrinae (Hymenoptera: Braconidae) species collected from two blocks of apple trees from 1993 to 1994 in southern Quebec, Canada. The habitat management block (HMB) had companion flowering plants within the block, whereas the control had no flowering plants. Thirty-six Microgastrinae species were identified including three new records for Canada: Apanteles tischeriae Viereck, Cotesia crambi Weed, Diolcogaster bakeri Muesebeck. The number of microgastrine varied considerably between the two blocks and years. Species numbers were similar between the two blocks, but the Shannon diversity and Evenness similarity indices indicated that HMB had a more diverse and balanced population of Microgastrinae. Matching our microgastrine identifications with literature records showed that the wasps could potentially parasitize over one-third of the lepidopteran pests of apples and/or other lepidopteran species recorded within this agro-ecosystem. The inclusion of four Asteraceae companion plant species in the HMB enhanced the diversity of Microgastrinae. 相似文献
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63.
Ihor E. Kopka Linus S. Lin James P. Jewell Thomas J. Lanza Tung M. Fong Chun-Pyn Shen Zhege J. Lao Sookhee Ha Laurie G. Castonguay Lex Van der Ploeg Mark T. Goulet William K. Hagmann 《Bioorganic & medicinal chemistry letters》2010,20(16):4757-4761
The design, synthesis, and binding activity of ring constrained analogs of the acyclic cannabinoid-1 receptor (CB1R) inverse agonist taranabant 1 are described. The initial inspiration for these taranabant derivatives was its conformation 1a, determined by 1H NMR, X-ray, and molecular modeling. The constrained analogs were all much less potent than their acyclic parent structure. The results obtained are discussed in the context of a predicted binding of 1 to a homology model of CB1R. 相似文献
64.
Sylvie Goulet Marc-André Poupart James Gillard Martin Poirier George Kukolj Pierre L. Beaulieu 《Bioorganic & medicinal chemistry letters》2010,20(1):196-200
Previously described SAR of benzimidazole-based non-nucleoside finger loop (Thumb Pocket I) inhibitors of HCV NS5B polymerase was expanded. Prospecting studies using parallel synthesis techniques allowed the rapid identification of novel cinnamic acid right-hand sides that provide renewed opportunities for further optimization of these inhibitors. Novel diamide derivatives such as 44 exhibited comparable potency (enzymatic and cell-based HCV replicon) as previously described tryptophan-based inhibitors but physicochemical properties (e.g., aqueous solubility and lipophilicity) have been improved, resulting in molecules with reduced off-target liabilities (CYP inhibition) and increased metabolic stability. 相似文献
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66.
Charles Goulet Catherine Goulet Marie‐Claire Goulet Dominique Michaud 《Proteomics》2010,10(13):2536-2544
We provide 2‐D gel reference maps for the apoplastic proteome of Nicotiana benthamiana leaves infiltrated or not with the bacterial gene vector Agrobacterium tumefaciens. About 90 proteins were analyzed by LC‐MS/MS for identification and function assignment. We show, overall, an effective response of the plant to agroinfiltration involving a specific, cell wall maintenance‐independent up‐regulation of defense protein secretion. The proteome maps described should be a useful tool for systemic studies on plant–pathogen interactions or cell wall metabolism. They also should prove useful for the monitoring of secreted recombinant proteins and their possible pleiotropic effects along the cell secretory pathway of N. benthamiana leaves used as an expression platform for clinically useful proteins. 相似文献
67.
Latkany P Duggal M Goulet J Paek H Rambo M Palmisano P Levin W Erdos J Justice A Brandt C 《Journal of ocular biology, diseases, and informatics》2010,3(1):30-34
We performed a validation study by chart review of data for exudative age-related macular degeneration (eAMD) and, because of the Veterans Administration (VA) therapy policy, ranibizumab usage in the largest electronic medical record system in the USA. We reviewed 5,854 distinct patients who visited an ophthalmology clinic within VA Connecticut from January 2006-December 2008. We randomly selected 98 of 138 distinct eAMD patients and 265 of 5,588 non-eAMD patients who did not receive ranibizumab. International Classification of Diseases, Ninth Revision, Clinical Modification coding of eAMD had an excellent positive predictive value of 97.8% (95% confidence interval (CI), 93.5-99.4%). The national Decision Support System (DSS) had an excellent positive predictive value of 100% (95% CI, 79.9-100%) for ranibizumab. However, the negative predictive value of the DSS dispensed ranibizumab decreased to 67.5 (95% CI, 62.1-72.4) because of a change in the way local values were stored that led to errors. Therefore, validation of clinical information over time in large databases is necessary. 相似文献
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69.
Byrum RS Goulet JL Snouwaert JN Griffiths RJ Koller BH 《Journal of immunology (Baltimore, Md. : 1950)》1999,163(12):6810-6819
Arachidonic acid metabolism by 5-lipoxygenase leads to production of the potent inflammatory mediators, leukotriene (LT) B4 and the cysteinyl LT. Relative synthesis of these subclasses of LT, each with different proinflammatory properties, depends on the expression and subsequent activity of LTA4 hydrolase and LTC4 synthase, respectively. LTA4 hydrolase differs from other proteins required for LT synthesis because it is expressed ubiquitously. Also, in vitro studies indicate that it possesses an aminopeptidase activity. Introduction of cysteinyl LT and LTB4 into animals has shown LTB4 is a potent chemoattractant, while the cysteinyl LT alter vascular permeability and smooth muscle tone. It has been impossible to determine the relative contributions of these two classes of LT to inflammatory responses in vivo or to define possible synergy resulting from the synthesis of both classes of mediators. To address this question, we have generated LTA4 hydrolase-deficient mice. These mice develop normally and are healthy. Using these animals, we show that LTA4 hydrolase is required for the production of LTB4 in an in vivo inflammatory response. We show that LTB4 is responsible for the characteristic influx of neutrophils accompanying topical arachidonic acid and that it contributes to the vascular changes seen in this model. In contrast, LTB4 influences only the cellular component of zymosan A-induced peritonitis. Furthermore, LTA4 hydrolase-deficient mice are resistant to platelet-activating factor, identifying LTB4 as one mediator of the physiological changes seen in systemic shock. We do not identify an in vivo role for the aminopeptidase activity of LTA4 hydrolase. 相似文献
70.
Brodeur I Goulet I Tremblay CS Charbonneau C Delisle MC Godin C Huard C Khandjian EW Buchwald M Lévesque G Carreau M 《The Journal of biological chemistry》2004,279(6):4713-4720
The function of the Fanconi anemia group C protein (FANCC) is still unknown, though many studies point to a role in damage response signaling. Unlike other known FA proteins, FANCC is mainly localized to the cytoplasm and is thought to act as a messenger of cellular damage rather than an effector of repair. FANCC has been shown to interact with several cytoplasmic and nuclear proteins and to delay the onset of apoptosis through redox regulation of GSTP1. We investigated the fate and function of FANCC during apoptosis. Here we show that FANCC undergoes proteolytic modification by a caspase into a predominant 47-kDa ubiquitinated protein fragment. Lack of proteolytic modification at the putative cleavage site delays apoptosis but does not affect MMC complementation. These results suggest that FANCC function is regulated through proteolytic processing. 相似文献