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181.
Effectiveness of weekly subcutaneous recombinant human erythropoietin administration for chemotherapy-induced anemia 总被引:6,自引:0,他引:6
Mamoru Tsukuda Seiichiro Yuyama Hidehiro Kohno Keiko Itoh Toshiyuki Kokatsu Satoshi Kawai 《Biotherapy》1998,11(1):21-25
The effects of weekly subcutaneousrecombinant human erythropoietin (r-hEPO)administration on anemia during chemotherapy includingcisplatin and 5-fluorouracil in patients with head andneck carcinomas were examined. Weekly subcutaneousr-hEPO administration in cancer patients has not beeninvestigated previously. Patients were treated withr-hEPO 100 IU/kg (2 patients), 200 IU/kg (6 patients),or 400 IU/kg (5 patients), or placebo, andeffectiveness was evaluated by monitoring hemoglobinconcentration changes after administration for 8weeks. Hemoglobin concentrations in all 3 r-hEPOdosage groups were higher than that in the controlgroup during chemotherapy. All r-hEPO doses producedimprovements in the anemia induced by chemotherapy;however, the 400 IU/kg dose was most effective. Therequirement for blood transfusions decreased inpatients receiving r-hEPO therapy, and no significantside-effects were associated with r-hEPOadministration. These results suggest thatchemotherapy-induced anemia can be prevented by weeklysubcutaneous r-hEPO administration. 相似文献
182.
Taro Kawai Makoto Matsumoto Kiyoshi Takeda Hideki Sanjo Shizuo Akira 《Molecular and cellular biology》1998,18(3):1642-1651
183.
184.
Seiji Sato Yoshifumi Saisho Kinsei Kou Shu Meguro Masami Tanaka Junichiro Irie Toshihide Kawai Hiroshi Itoh 《PloS one》2015,10(3)
Aims
To clarify the efficacy and safety of adding sitagliptin to insulin therapy in Japanese patients with suboptimally controlled type 2 diabetes (T2DM).Study Design and Methods
This was a 24-week, prospective, randomized, open-labeled, controlled trial. Patients with T2DM who were suboptimally controlled despite receiving at least twice daily injection of insulin were enrolled in the study. The patients were randomized to continuation of insulin treatment (Insulin group) or addition of sitagliptin 50 to 100 mg daily to insulin treatment (Ins+Sita group). The primary outcome was change in HbA1c at week 24.Results
Adding sitagliptin to insulin significantly reduced HbA1c from 7.9 ± 1.0% at baseline to 7.0 ± 0.8% at week 24 (P <0.0001), while there was no significant change in HbA1c in the Insulin group (7.8 ± 0.7% vs. 7.8 ± 1.1%, P = 0.32). The difference in HbA1c reduction between the groups was 0.9% (95% confidence interval, 0.4 to 1.5, P = 0.01). There was no significant weight gain in either group. Incidence of hypoglycemia was significantly reduced in the Ins+Sita group compared with the Insulin group. Treatment satisfaction was improved in the Ins+Sita group. Baseline HbA1c level and beta cell function were associated with the magnitude of reduction in HbA1c in the Ins+Sita group.Conclusion
Adding sitagliptin to insulin reduced HbA1c without weight gain or increase in hypoglycemia, and improved treatment satisfaction in Japanese patients with T2DM who were suboptimally controlled despite at least twice daily injection of insulin.Trial Registration
The University Hospital Medical Information Network (UMIN) Clinical Trials Registry UMIN000004678 相似文献185.
Megumi Sasatani Yanbin Xu Hidehiko Kawai Lili Cao Satoshi Tateishi Tsutomu Shimura Jianxiang Li Daisuke Iizuka Asao Noda Kanya Hamasaki Yoichiro Kusunoki Kenji Kamiya 《PloS one》2015,10(2)
The ubiquitin ligase RAD18 is involved in post replication repair pathways via its recruitment to stalled replication forks, and its role in the ubiquitylation of proliferating cell nuclear antigen (PCNA). Recently, it has been reported that RAD18 is also recruited to DNA double strand break (DSB) sites, where it plays novel functions in the DNA damage response induced by ionizing radiation (IR). This new role is independent of PCNA ubiquitylation, but little is known about how RAD18 functions after IR exposure. Here, we describe a role for RAD18 in the IR-induced DNA damage signaling pathway at G2/M phase in the cell cycle. Depleting cells of RAD18 reduced the recruitment of the DNA damage signaling factors ATM, γH2AX, and 53BP1 to foci in cells at the G2/M phase after IR exposure, and attenuated activation of the G2/M checkpoint. Furthermore, depletion of RAD18 increased micronuclei formation and cell death following IR exposure, both in vitro and in vivo. Our data suggest that RAD18 can function as a mediator for DNA damage response signals to activate the G2/M checkpoint in order to maintain genome integrity and cell survival after IR exposure. 相似文献
186.
187.
Eiichiro Ono Yu Homma Manabu Horikawa Satoshi Kunikane-Doi Haruna Imai Seiji Takahashi Yosuke Kawai Masaji Ishiguro Yuko Fukui Toru Nakayama 《The Plant cell》2010,22(8):2856-2871
We identified two glycosyltransferases that contribute to the structural diversification of flavonol glycosides in grapevine (Vitis vinifera): glycosyltransferase 5 (Vv GT5) and Vv GT6. Biochemical analyses showed that Vv GT5 is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (GAT), and Vv GT6 is a bifunctional UDP-glucose/UDP-galactose:flavonol-3-O-glucosyltransferase/galactosyltransferase. The Vv GT5 and Vv GT6 genes have very high sequence similarity (91%) and are located in tandem on chromosome 11, suggesting that one of these genes arose from the other by gene duplication. Both of these enzymes were expressed in accordance with flavonol synthase gene expression and flavonoid distribution patterns in this plant, corroborating their significance in flavonol glycoside biosynthesis. The determinant of the specificity of Vv GT5 for UDP-glucuronic acid was found to be Arg-140, which corresponded to none of the determinants previously identified for other plant GATs in primary structures, providing another example of convergent evolution of plant GAT. We also analyzed the determinants of the sugar donor specificity of Vv GT6. Gln-373 and Pro-19 were found to play important roles in the bifunctional specificity of the enzyme. The results presented here suggest that the sugar donor specificities of these Vv GTs could be determined by a limited number of amino acid substitutions in the primary structures of protein duplicates, illustrating the plasticity of plant glycosyltransferases in acquiring new sugar donor specificities. 相似文献
188.
A novel mutated acetolactate synthase gene conferring specific resistance to pyrimidinyl carboxy herbicides in rice 总被引:3,自引:0,他引:3
Acetolactate synthase (ALS) is the first common enzyme in the biosynthetic pathway of branched-chain amino acids. Mutations
of specific amino acids in ALS have been known to confer resistance to ALS-inhibiting herbicides such as sulfonylureas and
pyrimidinyl carboxy (PC) herbicides. However, mutations conferring exclusive resistance to PC have not yet been reported to
date. We selected PC resistant rice calli, which were derived from anther culture, using one of the PCs, bispyribac-sodium
(BS), as a selection agent. Two lines of BS-resistant plants carrying a novel mutation, the 95th Glycine to Alanine (G95A),
in ALS were obtained. In vitro ALS activity assay indicated that the recombinant protein of G95A-mutated ALS (ALS-G95A) conferred
highly specific resistance to PC herbicides. In order to determine if the ALS-G95A gene could be used as a selection marker
for rice transformation, the ALS-G95A gene was connected to ubiquitin promoter and introduced into rice. PC resistant plants
containing integrated ALS-G95A gene were obtained after selection with BS as a selection agent. In conclusion, novel G95A
mutated ALS gene confers highly specific resistant to PC-herbicides and can be used as a selection marker. 相似文献
189.
Kawamoto Y Shotake T Nozawa K Kawamoto S Tomari K Kawai S Shirai K Morimitsu Y Takagi N Akaza H Fujii H Hagihara K Aizawa K Akachi S Oi T Hayaishi S 《Primates; journal of primatology》2007,48(1):27-40
We investigated the diversity and phylogeography of mitochondrial DNA (mtDNA) in Japanese macaques (Macaca fuscata), an endemic species in Japan that has the northernmost distribution of any non-human primate species. DNA samples from 135
localities representing the entire range of this species were compared. A total of 53 unique haplotypes were observed for
the 412-bp partial mtDNA control region sequence, with length variation distinguishing the two subspecies. Clustering analyses
suggested two putative major haplogroups, of which one was geographically distributed in eastern Japan and the other in western
Japan. The populations in the east showed lower mtDNA diversity than those in the west. Phylogeographical relationships of
haplotypes depicted with minimum spanning network suggested differences in population structure. Population expansion was
significant for the eastern but not the western population, suggesting establishment of the ancestral population was relatively
long ago in the west and recent in the east. Based on fossil evidence and past climate and vegetation changes, we inferred
that the postulated population expansion may have taken place after the last glacial period (after 15,000 years ago). Mitochondrial
DNA showed contrasting results in both variability and phylogenetic status of local populations to those of previous studies
using protein variations, particularly for populations in the periphery of the range, with special inference on habitat change
during the glacial period in response to cold adaptation.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
190.