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431.
D. Egron C. Perigaud A.-M. Aubertin J.-L. Imbach G. Gosselin 《Nucleosides, nucleotides & nucleic acids》2013,32(9-11):1725-1729
Abstract The synthesis and the anti-HIV activity of two mononucleoside phosphotriester derivatives of 3′-azido-2′,3′-dideoxythymidine (AZT) and 2′,3′-dideoxyadenosine (ddA) incorporating a new kind of phosphate protecting group, namely S-pivaloyl-4-thiobutyl (tBuSATB), are reported. 相似文献
432.
A G Loi A Faraj C Pierra G Gosselin J L Imbach S A Locarnini E V Groman R F Schinazi J P Sommadossi 《Nucleosides & nucleotides》1999,18(4-5):1005-1006
We have investigated the effects of several beta-D-ddA 5'-monophospate (beta-D-ddAMP), and their corresponding beta-L-enantiomers prodrugs against HBV replication. All ddAMP prodrugs inhibited HBV replication in a dose-dependent manner. 相似文献
433.
G Maga M Amacker U Hübscher G Gosselin J L Imbach C Mathé A Faraj J P Sommadossi S Spadari 《Nucleosides & nucleotides》1999,18(4-5):795-805
We have compared the HIV-1 RT mutants containing the single substitutions L100I, K103N, V106A, V179D, Y181I and Y188L, known to confer NNI-resistance in treated patients, to HIV-1 RT wt for their sensitivity towards inhibition by D- and L-deoxy- and dideoxy-nucleoside tiphosphates. The results showed a differential effect of the substitutions on the affinity for both D- and L-enantiomers of deoxy- and dideoxy-nucleoside triphosphates and provide a rationale for the utilization of L-dideoxynucleoside analogs with NNI in combination chemotherapy. 相似文献
434.
Sinorhizobium meliloti is a soil bacterium accumulating glutamate, N-acetylglutaminyl glutamine amide and trehalose in hyperosmolarity. Besides these compatible solutes, we highlighted several compounds in S. meliloti Rm1021 wild-type strain. The purification and the structural characterization based on liquid chromatography evaporative light scattering detector, electrospray ionization high resolution mass spectrometry and nuclear magnetic resonance techniques showed they were four linear oligosaccharides composed of 3, 4, 5 and 6 glucose units all linked by α-(1 → 2) linkages except a terminal α-(1 ↔ 1) linkage. These oligosaccharides were cytoplasmic and were observed in several wild-type strains suggesting they were common features in S. meliloti strains grown in hyperosmolarity. 相似文献
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A smooth Gaussian random field with zero mean and unit varianceis sampled on a discrete lattice, and we are interested in theexceedance probability or P-value of the maximum in a finiteregion. If the random field is smooth relative to the mesh size,then the P-value can be well approximated by results for thecontinuously sampled smooth random field (Adler, 1981; Worsley,1995a; Taylor & Adler, 2003; Adler & Taylor, 2007).If the random field is not smooth, so that adjacent latticevalues are nearly independent, then the usual Bonferroni boundis very accurate. The purpose of this paper is to bridge thegap between the two, and derive a simple, accurate upper boundfor intermediate mesh sizes. The result uses a new improvedBonferroni-type bound based on discrete local maxima. We givean application to the bubbles technique for detectingareas of the face used to discriminate fear from happiness. 相似文献
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440.
Identification of murine natural killer cell subsets with monoclonal antibodies derived from 129 anti-C57BL/6 immune spleen cells 总被引:3,自引:0,他引:3
S Lemieux F Ouellet-Talbot Y Lusignan L Morelli N Labrèche P Gosselin J Lecomte 《Cellular immunology》1991,134(1):191-204
Two hybridomas producing monoclonal antibodies reactive with natural killer cells were selected after fusion of 129 anti-C57BL/6 immune spleen cells with P3X63-Ag8.653 myeloma cells. Treatment of normal or stimulated cells with the 4LO3311 or the 4LO439 mAb and rabbit complement inhibited natural killer and antibody-dependent cellular cytotoxicities, whereas cell lysis mediated by natural cytotoxic cells, cytotoxic T lymphocytes, or activated macrophages was unaffected. Lymphokine-activated killer activity was reduced after complement-mediated treatment of interleukin-2-stimulated spleen cells with the 4LO3311 mAb but not after treatment with the 4LO439 mAb. Similar treatment of spleen cells with either mAb had no effect on the mitogen-induced proliferation of T and B lymphocytes and did not alter the frequency of antibody plaque-forming cells in immune spleen cell suspensions. The 4LO3311 and 4LO439 mAbs thus appear to be specific for NK cells and their progeny. Flow cytometry analysis confirmed that 4LO3311+ and 4LO439+ cells are phenotypically identical to NK-1.1+ cells. The epitope recognized by the 4LO3311 mAb has the same strain distribution as the NK-2.1 alloantigen previously detected with NZB anti-BALB/c antiserum, whereas the 4LO439 mAb appears to identify a new NK cell marker exclusively expressed in mice of C57BL lineage. The relationship of the molecules detected with either the 4LO3311 or the 4LO439 mAb to polymorphic antigens of the Ly series is discussed. 相似文献