全文获取类型
收费全文 | 8453篇 |
免费 | 819篇 |
国内免费 | 3篇 |
出版年
2021年 | 101篇 |
2020年 | 70篇 |
2019年 | 101篇 |
2018年 | 87篇 |
2017年 | 74篇 |
2016年 | 125篇 |
2015年 | 235篇 |
2014年 | 232篇 |
2013年 | 320篇 |
2012年 | 403篇 |
2011年 | 398篇 |
2010年 | 271篇 |
2009年 | 232篇 |
2008年 | 361篇 |
2007年 | 307篇 |
2006年 | 308篇 |
2005年 | 321篇 |
2004年 | 318篇 |
2003年 | 283篇 |
2002年 | 294篇 |
2001年 | 178篇 |
2000年 | 138篇 |
1999年 | 153篇 |
1998年 | 112篇 |
1997年 | 100篇 |
1996年 | 107篇 |
1995年 | 75篇 |
1994年 | 75篇 |
1993年 | 89篇 |
1992年 | 133篇 |
1991年 | 129篇 |
1990年 | 135篇 |
1989年 | 112篇 |
1988年 | 102篇 |
1987年 | 111篇 |
1986年 | 108篇 |
1985年 | 133篇 |
1984年 | 103篇 |
1983年 | 110篇 |
1982年 | 103篇 |
1981年 | 92篇 |
1980年 | 100篇 |
1979年 | 75篇 |
1978年 | 84篇 |
1977年 | 92篇 |
1976年 | 67篇 |
1974年 | 91篇 |
1973年 | 67篇 |
1972年 | 78篇 |
1971年 | 68篇 |
排序方式: 共有9275条查询结果,搜索用时 31 毫秒
141.
A model of the smooth pursuit eye movement system 总被引:18,自引:0,他引:18
Human, horizontal, smooth-pursuit eye movements were recorded by the search coil method in response to Rashbass step-ramp stimuli of 5 to 30 deg/s. Eye velocity records were analyzed by measuring features such as the time, velocity and acceleration of the point of peak acceleration, the time and velocity of the peaks and troughs of ringing and steady-state velocity. These values were averaged and mean responses reconstructed. Three normal subjects were studied and their responses averaged. All showed a peak acceleration-velocity saturation. All had ringing frequencies near 3.8 Hz and the mean steady-state gain was 0.95.It is argued that a single, linear forward path with any transfer function G(s) and a 100 ms delay (latency) cannot simultaneously simulate the initial rise of acceleration and ring at 3.8 Hz based on a Bode analysis. Also such a simple negative feedback model cannot have a steady-state gain greater than 1.0; a situation that occurs frequently experimentally. L.R. Young's model, which employs internal positive feedback to eliminate the built-in unity negative feedback, was felt necessary to resolve this problem and a modification of that model is proposed which simulates the data base. Acceleration saturation is achieved by borrowing the idea of the local feedback model for saccades so that one nonlinearity can account for the acceleration-velocity saturation: the main sequence for pursuit. Motor plasticity or motor learning, recently demonstrated for pursuit, is also incorporated and simulated.It was noticed that the offset of pursuit did not show the ringing seen in the onset so this was quantified in one subject. Offset velocity could be characterized by a single exponential with a time constant of about 90 ms. This observation suggests that fixation is not pursuit at zero velocity and that the pursuit system is turned on when needed and off during fixation. 相似文献
142.
Gordon Bull 《Coral reefs (Online)》1986,4(3):197-200
High densities of eggs and larvae of scleractinian corals were found in plankton samples after mass, multi-specific spawnings on inshore high island fringing reefs in the central Great Barrier Reef region. Immediately after spawning, vertical stratification was observed with eggs concentrated on the surface. Larvae were found to be distributed vertically and horizontally within 12 hours of spawning. Larval development over the subsequent 6–7 days was associated with an increase in the length/width ratios of larvae and their volumes. Influxes of mature larvae onto the study reef were observed 3–6 days after spawning. The relative success of the sampling in relation to other reports in the literature, and the future for more informed work on the larval ecology of corals are discussed. 相似文献
143.
144.
Microbial fermentative preparation of L-[15N2]lysine and its tracer: application to serum amino acid kinetic studies 总被引:1,自引:0,他引:1
C S Irving C L Cooney L T Brown D Gold J Gordon P D Klein 《Analytical biochemistry》1983,131(1):93-98
The microorganism Brevibacterium flavum 21129 has been used to produce multigram batches of L-[15N2]lysine of high purity and isotopic enrichment by supplementation of the growth medium with (15NH4)2SO4 of 98.0 atom% excess. The doubly 15N-labeled lysine can be detected at dilutions 10 times greater than singly labeled lysine when isotope dilution curves are analyzed by gas chromatography-mass spectrometry. This enhanced sensitivity permits kinetic measurements of plasma free-lysine isotope content over a 300-fold dilution during 6 h following a single oral bolus of 5 mg/kg body wt. This inexpensive preparation method lends itself to the production of highly useful biochemical compounds for kinetic studies of human nutrition. 相似文献
145.
Further evidence for the protein coding potential of the mouse mammary tumor virus long terminal repeat: nucleotide sequence of an endogenous proviral long terminal repeat 总被引:18,自引:11,他引:7 下载免费PDF全文
The 3′ half of an endogenous mouse mammary tumor virus from a C3H mouse was cloned in the Charon 4A vector phage. A comparison of the proviral clone with previously published endogenous mouse mammary tumor virus restriction maps identified it as endogenous unit II (J. Cohen and H. Varmus, Nature [London] 278:418-423, 1979), which is present in all inbred mouse strains derived from the original Bagg albino × DBA cross. The nucleotide sequence of the unit II long terminal redundancy (LTR) was determined and compared with the sequence previously determined for the exogenous C3H virus LTR (Donehower et al., J. Virol. 37:226-238, 1981). Virtually all sequence differences between the two LTRs were base substitutions. The total amount of sequence divergence was 6.6%. The large open reading frame reported previously in the exogenous LTR was preserved in the endogenous LTR. In addition, the pattern of sequence divergence was highly nonrandom with respect to the putative amino acid codons of the two open reading frames. Most of the base substitutions in this region resulted in silent or conservative amino acid codon changes. The nonrandom divergence pattern indicates that selective forces are operating on this segment of DNA and argues that the putative protein is functional in the life cycle of mouse mammary tumor virus. Possible roles for the protein and its mode of expression are discussed. 相似文献
146.
Spatial and temporal properties of luminosity horizontal cells in the turtle retina 总被引:7,自引:6,他引:1 下载免费PDF全文
Luminosity horizontal cells in the turtle retina respond approximately linearly to visual stimuli with contrast levels spanning a large part of the physiological range. We characterized the response properties of these cells under conditions of low photopic background illumination by measuring their spatial and temporal frequency transfer functions. Our experimental results indicate in two ways that, under these conditions, feedback from luminosity horizontal cells to cones does not play a major role in the mechanisms underlying the spatial and temporal tuning of horizontal cell responses. First, the shape of the spatial transfer function depended only weakly on the temporal frequency with which it was measured. Second, the shape of the temporal transfer function depended only weakly on the spatial frequency with which it was measured. 相似文献
147.
Thermotropic lipid phase separations in human platelet and rat liver plasma membranes 总被引:2,自引:0,他引:2
Larry M. Gordon Patrick W. Mobley Judy A. Esgate Gary Hofmann Anthony D. Whetton Miles D. Houslay 《The Journal of membrane biology》1983,76(2):139-149
Electron spin resonance (ESR) studies were conducted on human platelet plasma membranes using 5-nitroxide stearate, I(12,3). The polarity-corrected order parameter S and polarity-uncorrected order parameters S(T parallel) and S(T perpendicular) were independent of probe concentration at low I(12.3)/membrane protein ratios. At higher ratios, S and S(T perpendicular) decreased with increasing probe concentration while S(T parallel) remained unchanged. This is the result of enhanced radical interactions due to probe clustering. A lipid phase separation occurs in platelet membranes that segregates I(12,3) for temperatures less than 37 degrees C. As Arrhenius plots of platelet acid phosphatase activity exhibit a break at 35 to 36 degrees C, this enzyme activity may be influenced by the above phase separation. Similar experiments were performed on native [cholesterol/phospholipid ratio (C/P) = 0.71] and cholesterol-enriched [C/P = 0.85] rat liver plasma membranes. At 36 degrees C, cholesterol loading reduces I(12,3) flexibility and decreases the probe ratio at which radical interactions are apparent. The latter effects are attributed to the formation of cholesterol-rich lipid domains, and to the inability of I(12,3) to partition into these domains because of steric hinderance. Cholesterol enrichment increases both the high temperature onset of the phase separation occurring in liver membranes from 28 degrees to 37 degrees C and the percentage of probe-excluding, cholesterol-rich lipid domains at elevated temperatures. A model is discussed attributing the lipid phase separation in native liver plasma membranes to cholesterol-rich and -poor domains. As I(12,3) behaves similarly in cholesterol-enriched liver and human platelet plasma membranes, cholesterol-rich and -poor domains probably exist in both systems at physiologic temperatures. 相似文献
148.
Day 7 to 9 embryos were frozen by a rapid two-step method to ?38°C before being plunged into liquid nitrogen. Glycerol was used as the cryoprotectant and, following thawing, the embryos were cultured for 12 – 24 hours in PBS + 15% heat-treated steer serum. In Experiment 1, embryos were frozen in 2.0 ml glass ampoules or 0.5 ml Cassou straws. Two levels of glycerol (1.0M and 1.4M) gave comparable survival rates ( and , respectively). A greater proportion of embryos developed in culture after freezing in straws. In Experiment 2, embryos were classified morphologically before and after freezing into 5 grades (1 = excellent; 2 = good; 3 = fair; 4 = poor; 5 = degenerate). Only embryos of grade 1, 2 and 3 were frozen. The post-thaw survival rates for embryos graded 1, 2 and 3 before freezing were 100% (), 86% () and 83% (), respectively. Furthermore, the porportion of surviving embryos estimated to be of poor quality (grade 4) was greater for embryos graded 3 before freezing () than for embryos graded 2 () or 1 (). The percentage of embryos which developed normally after culture for each of the pre-freezing grades 1, 2 and 3 was 91% (), 50% () and 29% (), respectively. Of the total number of frozen-thawed embryos which developed in culture, (16%) were of poor quality. The proportion of poor quality developing embryos was greater inembryos graded 3 before freezing () than those graded 2 (). All of the embryos graded 1 before freezing and which developed in culture were of good quality. Results indicate that, if high post-thaw survival rates are to be obtained, stringent embryo selection processes will be required. 相似文献
149.
We have constructed two miniplasmids, derived from the resistance plasmid R100.1. In one of these plasmids 400 bp of R100.1 DNA have been replaced by DNA from the transposon Tn1000 (gamma-delta). This substitution removes the amino-terminal end of the repA2 coding sequence of R100.1 and results in an increased copy number of the plasmid carrying the substitution. The copy number of the substituted plasmid is reduced to normal levels in the presence of R100.1. The repA2 gene thus encodes a trans-acting repressor function involved in the control of plasmid replication. 相似文献
150.
Levels of immunoreactive pro-opiomelanocortin (POMC) peptides (N- and C-terminal ACTH, N- and C-terminal LPH and α-MSH) have been measured in pituitary extracts from human fetuses of 12–22 weeks gestation. The levels of ACTH were 30–200 times higher than α-MSH in all fetuses studied. Sephadex G-75 and G-25 chromatography of 8 extracts showed peaks of 34 kilodaltons (K) POMC, 22K ACTH, β-LPH, γ-LPH, β-endorphin, approximately 8K ACTH, 1–39 ACTH, α-MSH and CLIP. The 8K and 22K forms of ACTH are both partly glycosylated.In vitro culture of pituitaries from 2 fetuses (22 and 26 weeks gestation) gave a detectable basal output of ACTH but not of α-MSH. Stimulation of these pituitary cells with human fetal and rat hypothalamic extracts and with synthetic ovine CRF-41 produced a significant increase in ACTH release, and either small or undetectable amounts of α-MSH.These results demonstrate the presence of POMC-related peptides in early gestation human fetal pituitaries and suggest that ACTH, and not α-MSH, is the major corticotrophic hormone at this stage of gestation. 相似文献