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31.
Neetha John Moka Rajasekhar Katta Mohan Girisha Podila Satya Venkata Narasimha Sharma Puthiya Mundyat Gopinath 《Indian journal of human genetics》2013,19(2):165-170
BACKGROUND:
Mental retardation (MR) is a heterogeneous dysfunction of the central nervous system exhibiting complex phenotypes and has an estimated prevalence of 1-3% in the general population. However, in about 50% of the children diagnosed with any form of intellectual disability or developmental delay the cause goes undetected contributing to idiopathic intellectual disability.MATERIALS AND METHODS:
A total of 122 children with developmental delay/MR were studied to identify the microscopic and submicroscopic chromosome rearrangements by using the conventional cytogenetics and multiplex ligation dependent probe amplification (MLPA) analysis using SALSA MLPA kits from Microbiology Research Centre Holland [MRC] Holland.RESULTS:
All the recruited children were selected for this study, after thorough clinical assessment and metaphases prepared were analyzed by using automated karyotyping system. None was found to have chromosomal abnormality; MLPA analysis was carried out in all subjects and identified in 11 (9%) patients.CONCLUSION:
Karyotype analysis in combination with MLPA assays for submicroscopic micro-deletions may be recommended for children with idiopathic MR. 相似文献32.
Sutendra G Wong S Fraser ME Huber RE 《Biochemical and biophysical research communications》2007,352(2):566-570
It is shown here that Escherichia coli beta-galactosidase has a second Mg2+ binding site that is important for activity. Binding of Mg2+ to the second site caused the k(cat) (with oNPG as the substrate) to increase about 100 s(-1); the Km was not affected. The Kd for binding the second Mg2+ is about 10(-4)M. Since the concentration of free Mg2+ in E. coli is about 1-2 mM, the second site is physiologically significant. Non-polar substitutions (Ala or Leu) for Glu-797, a residue in an active site loop, eliminated the k(cat) increase. This indicates that the second Mg2+ site is near to Glu-797. The Ki values of transition state analogs were decreased by small but statistically significant amounts when the second Mg2+ site was occupied and Arrhenius plots showed that less entropic activation energy is required when the second site is occupied. These inhibitor and temperature results suggest that binding of the second Mg2+ helps to order the active site for stabilization of the transition state. 相似文献
33.
Marimuthu Citartan Thean-Hock Tang Soo-Choon Tan Subash C. B. Gopinath 《World journal of microbiology & biotechnology》2011,27(5):1167-1173
The generation of DNA aptamer by Systematic Evolution of Ligands by Exponential Enrichment requires a good method of ssDNA
generation. There are various methods developed to generate ssDNA such as streptavidin-biotin based separation techniques,
asymmetric PCR and strand separation of the PCR product containing primer with a terminator and an extension of 20 nucleotides
on denaturing urea-polyacrylamide gel. In the present investigation, we have shown the possible improvements for the regular
lambda nuclease digestion under optimized conditions. Optimization of the PCR cycles, time course studies on lambda nuclease
digestion and purification of the ssDNA from the lambda exonuclease digestion mixture was found to be able to recover ssDNA
amounting up to 39.19 ± 2.48 % of the starting amount of dsDNA. These strategies can be applied to the techniques involving
essential usage of ssDNA. 相似文献
34.
Gopinath KP Kathiravan MN Srinivasan R Sankaranarayanan S 《Bioresource technology》2011,102(4):3687-3693
In this study, it was attempted to evaluate the influences and also recommended some elimination methods for inhibitory effects offered by salts and heavy metal ions. Congo red dye solution treated with mutant Pseudomonas sp. was taken as a model system for study. The salts used in this study are NaCl, CaCl2 and MgSO4·7H2O. Though the growth was inhibited at concentrations above 4 g/l, toleration was achieved by acclimatization process. In case of heavy metal ions, Cr (VI) showed low inhibition up to 500 mg/l of concentration, compared to Zn (II) and Cu (II). It was due to the presence of chromium reductase enzyme which was confirmed by SDS-PAGE. Zn (II) and Cu (II) ion inhibitions were eliminated by chelation with EDTA. The critical ion concentrations obtained as per Han-Levenspiel model for Cr (VI), Zn (II) and Cu (II) were 0.8958, 0.3028 and 0.204 g/l respectively. 相似文献
35.
36.
T. S. Kumarevel S. C. B. Gopinath A. Hilda N. Gautham M. N. Ponnusamy 《World journal of microbiology & biotechnology》2005,21(1):23-26
Lipase from the oil-mill waste isolate Cunninghamella verticillata was purified by stepwise precipitation using acetone, as a sequel to our earlier conventional column chromatographic method [Gopinath et al. (2002)World Journal of Microbiology and Biotechnology 18, 449–458]. The yield of purified lipase was approx. 4-fold higher than by the previous method and the purified lipase was obtained with 70–80% acetone saturations. The enzyme was resolved as a single band with homogeneity both by native and by SDS–PAGE. The optimum condition for the lipase to crystallize was 5 g of enzyme in 0.05 M sodium phosphate buffer (pH 6.5) with 5 mM FeCl2 and 10% 2-methyl 2,4-pentanediol (MPD).These authors equally contributed to this work 相似文献
37.
Six variants of P12, a Kazal-type trypsin inhibitor in the secretion of male mouse accessory sexual glands, were made using single-site mutations including R19L, Y21V, D22G, R43G, K44S, and R45T, based on one-letter-code mutation of amino acids. The other two variants, Nd10 and Cd8, were made using the deletion of 10 and 8 residues from the N- and C-terminals, respectively. Their CD profiles revealed maintenance of the P12 conformation in the seven variants, excluding Cd8, which became unfolded. Only R19L entirely lost the ability while the other variants were as strong as P12 in inhibiting the trypsin digestion of N-benzoyl-Phe-Val-Arg 7-amido-4-methylcoumarin. The immunocytochemical results demonstrated that D22G and Cd8 failed to bind to sperm, Y21V very weakly did so, and the other variants retained their sperm-binding abilities. Concomitantly, the immunocytochemical stainability of each ligand was parallel to its inhibitory effect on 125I-P12-sperm binding, and a synthetic oligopeptide corresponding to residues 18-24 of P12 was able to inhibit P12-sperm binding. The data together concluded that R19 was essential for protease inhibition and D22 and/or Y21 mainly being responsible for the binding of P12 to sperm. The steric arrangement of R19, Y21, and D22 on the tertiary structure of P12 is discussed. 相似文献
38.
Aptamers are known for their higher discriminating ability between closely related molecules and their requirement for only a small region for binding, as compared to an antibody. In the present studies, we have isolated a specific RNA aptamer against the influenza virus B/Johannesburg/05/1999 by an in vitro selection procedure. The aptamer bound efficiently to the HA of influenza B and required 5 mM MgCl(2) ion for its recognition. The aptamer not only distinguished HA derived from the influenza A virus, but also inhibited HA-mediated membrane fusion. 相似文献
39.
Gopinath Balakrish Nair Thandavarayan Ramamurthy Dipika Sur Takashi Kurakawa Takuya Takahashi Koji Nomoto Yoshifumi Takeda 《Microbiology and immunology》2012,56(11):789-791
During a double‐blind, randomized, placebo‐controlled probiotic trial among 3758 children residing in an urban slum in Kolkata, India, Vibrio cholerae/mimicus was detected in fecal microbiota of healthy children. The importance of this finding in the local, regional and global transmission of cholera is discussed. 相似文献
40.
G. Gopinath A. K. Mahapatra J. C. Bharadwaj R. Banerji D. N. Sharma P. N. Tandon 《Journal of biosciences》1989,14(3):255-260
A feasibility study of neural transplantation in adult rhesus monkey was undertaken. Fresh and preserved neocortex containing
multiplying and maturing neurons obtained from 55–70 gestation days were transplanted into the striatum, cerebellum and cerebral
cortex of adult monkeys. Tissues were preserved for 4 days either at subzero temperature in the freezer compartment of the
ordinary refrigerator in Ringer lactate or incubated in culture medium. While 2 monkeys out of 5 injected with preserved tissue
had successful transplants after 4 months, all the 10 monkeys injected with fresh tissue had no transplants. The size of the
two surviving transplants was small. The neurons in the transplants were mainly in clusters. Many of the cells were immature
and some showed early degenerative changes. Neuronal processes were restricted to the transplants and thus showed lack of
morphological integration with the host tissue. Further studies are in progress to define the nature of the embryonic tissue
of primate which can grow and survive and also the role of neural grafts in functional recovery following experimental lesions
of the brain regions. 相似文献