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141.
Exercise induces isoform-specific increase in 5'AMP-activated protein kinase activity in human skeletal muscle 总被引:21,自引:0,他引:21
Fujii N Hayashi T Hirshman MF Smith JT Habinowski SA Kaijser L Mu J Ljungqvist O Birnbaum MJ Witters LA Thorell A Goodyear LJ 《Biochemical and biophysical research communications》2000,273(3):1150-1155
The 5'AMP-activated protein kinase (AMPK) is stimulated by contractile activity in rat skeletal muscle. AMPK has emerged as an important signaling intermediary in the regulation of cell metabolism being linked to exercise-induced changes in muscle glucose and fatty acid metabolism. In the present study, we determined the effects of exercise on isoform-specific AMPK activity (alpha1 and alpha2) in human skeletal muscle. Needle biopsies of vastus lateralis muscle were obtained from seven healthy subjects at rest, after 20 and 60 min of cycle ergometer exercise at 70% of VO(2)max, and 30 min following the 60 min exercise bout. In comparison to the resting state, AMPK alpha2 activity significantly increased at 20 and 60 min of exercise, and remained at a higher level with 30 min of recovery. AMPK alpha1 activity tended to slightly decrease with 20 min of exercise at 70%VO(2)max; however, the change was not statistically significant. AMPK alpha1 activities were at basal levels at 60 min of exercise and 30 min of recovery. On a separate day, the same subjects exercised for 20 min at 50% of VO(2)max. Exercise at this intensity did not change alpha2 activity, and similar to exercise at 70% of VO(2)max, there was no significant change in alpha1 activity. In conclusion, exercise at a higher intensity for only 20 min leads to increases in AMPK alpha2 activity but not alpha1 activity. These results suggest that the alpha2-containing AMPK complex, rather than alpha1, may be involved in the metabolic responses to exercise in human skeletal muscle. 相似文献
142.
Loss of class IA PI3K signaling in muscle leads to impaired muscle growth, insulin response, and hyperlipidemia 总被引:3,自引:0,他引:3
Luo J Sobkiw CL Hirshman MF Logsdon MN Li TQ Goodyear LJ Cantley LC 《Cell metabolism》2006,3(5):355-366
The evolutionarily conserved phosphoinositide 3-kinase (PI3K) signaling pathway mediates both the metabolic effects of insulin and the growth-promoting effects of insulin-like growth factor-1 (IGF-1). We have generated mice deficient in both the p85alpha/p55alpha/p50alpha and the p85beta regulatory subunits of class I(A) PI3K in skeletal muscles. PI3K signaling in the muscle of these animals is severely impaired, leading to a significant reduction in muscle weight and fiber size. These mice also exhibit muscle insulin resistance and whole-body glucose intolerance. Despite their ability to maintain normal fasting and fed blood glucose levels, these mice show increased body fat content and elevated serum free fatty acid and triglyceride levels. These results demonstrate that in vivo p85 is a critical mediator of class I(A) PI3K signaling in the regulation of muscle growth and metabolism. Our finding also indicates that compromised muscle PI3K signaling could contribute to symptoms of hyperlipidemia associated with human type 2 diabetes. 相似文献
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The self-incompatibility phenotype in brassica is altered by the transformation of a mutant S locus receptor kinase 总被引:2,自引:0,他引:2 下载免费PDF全文
The self-incompatible (SI) Brassica napus line W1, which carries the 910 S allele, was transformed with an inactive copy of the 910 S locus receptor kinase (SRK) gene. Two transformed lines were analyzed based on their heritable ability to set self-seed. The first line was virtually completely self-compatible (SC), and reciprocal pollinations with the original W1 line demonstrated that only the stigma side of the SI phenotype was altered. An analysis of the expression of endogenous SRK-910 demonstrated that the mechanism of transgene action is via gene suppression. Furthermore, the expression of the S locus glycoprotein gene present in the 910 allele (SLG-910), SLG-A10, which is derived from a nonfunctional S allele, and an S locus-related gene were also suppressed. When the transgene was crossed into another SI line carrying the A14 S allele, it was also capable of suppressing the expression of the endogenous genes and of making this line SC. The second transgenic line studied was only partly SC. In this case as well, only the stigma phenotype was affected, although no gene suppression was detected for endogenous SRK-910 or SLG-910. In this line, the expression of the transgene most likely was causing the change in phenotype, and no effect was observed when this transgene was crossed into the other SI line. Therefore, this work reinforces the hypothesis that the SRK gene is required, but only for the stigma side of the SI phenotype, and that a single transgene can alter the SI phenotype of more than one S allele. 相似文献
145.
Karl A. Dunne Amr Allam Anne McIntosh Stephanie A. Houston Vuk Cerovic Carl S. Goodyear Andrew J. Roe Scott A. Beatson Simon W. Milling Daniel Walker Daniel M. Wall 《PloS one》2013,8(7)
Adherent invasive Escherichia coli (AIEC) have been implicated as a causative agent of Crohn’s disease (CD) due to their isolation from the intestines of CD sufferers and their ability to persist in macrophages inducing granulomas. The rapid intracellular multiplication of AIEC sets it apart from other enteric pathogens such as Salmonella Typhimurium which after limited replication induce programmed cell death (PCD). Understanding the response of infected cells to the increased AIEC bacterial load and associated metabolic stress may offer insights into AIEC pathogenesis and its association with CD. Here we show that AIEC persistence within macrophages and dendritic cells is facilitated by increased proteasomal degradation of caspase-3. In addition S-nitrosylation of pro- and active forms of caspase-3, which can inhibit the enzymes activity, is increased in AIEC infected macrophages. This S-nitrosylated caspase-3 was seen to accumulate upon inhibition of the proteasome indicating an additional role for S-nitrosylation in inducing caspase-3 degradation in a manner independent of ubiquitination. In addition to the autophagic genetic defects that are linked to CD, this delay in apoptosis mediated in AIEC infected cells through increased degradation of caspase-3, may be an essential factor in its prolonged persistence in CD patients. 相似文献
146.
Goodyear CS Sugiyama F Silverman GJ 《Journal of immunology (Baltimore, Md. : 1950)》2006,176(4):2262-2271
The effective functioning of the adaptive immune system requires careful clonal regulation within the B cell compartment. Some microbial pathogens produce virulence factors, like staphylococcal protein A, which interact at high frequencies with B lymphocyte through unconventional binding sites in BCR variable region frameworks conserved during evolution. We have characterized the in vivo effect of staphylococcal protein A treatment on peripheral B cells bearing susceptible BCR, and found a dose-dependent direct relationship over the range of 2 mg to <0.2 microg in the magnitude of induced BCR-targeted supraclonal cell death. Significantly, some level of targeted B cell proliferation was always detectable, with greatest interim supraclonal expansion demonstrated at 2 days after 20-microg treatment. Subsequently, this transient expansion always collapsed. In direct comparisons, i.p. treatment was more efficacious than i.v. treatment, although at higher doses this finding was less marked. These studies elucidate a general paradigm in which in vivo encounters with a B cell superantigen are uniformly associated with proliferative expansion followed by deletion that is more rapid and complete with higher doses, whereas lower doses lead to greater transient in vivo expansion with delayed deletion to levels at later times that are still quantitatively proportional to the dose. Our results document the potent in vivo B cell-targeted properties of a microbial B cell superantigen, even at submicrogram doses associated with great molar excess of circulating Ig, and clearly illustrate the intertwined relationships between targeted proliferative cycling and apoptotic death that is induced by a microbial B cell superantigen. 相似文献
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149.
Jacinta Guirguis Luke E. B. Goodyear Catherine Finn Jack V. Johnson Daniel Pincheira-Donoso 《Global Ecology and Biogeography》2023,32(11):1952-1963
Aim
Life in mountains is associated with multiple features that increase the risk of demographic collapses in populations – small geographic ranges, short breeding seasons, specialization to harsh climates – leading to the hypothesis that extinction risk is exacerbated in species inhabiting higher elevations. Here, we implement the first test of this hypothesis across the amphibian tree of life – the tetrapods with the largest proportion of montane species, and nature's most threatened animals.Location
Global.Time Period
Present.Major Taxa Studied
Class Amphibia.Methods
We collated a dataset spanning 8042 species from across all three amphibian orders (Anura, Caudata and Gymnophiona). We preformed phylogenetic logistic regressions to test the predictions that extinction risk increases with elevation, and whether this effect is caused by factors previously hypothesised to drive high-elevation declines, including restrictions on species' geographic ranges, variation in their life histories and the presence of infectious disease.Results
Globally, extinction risk increases towards higher elevations. At order-level, this relationship holds for frogs and salamanders. Even when controlling for geographic range size, life histories and infectious disease, extinction risk increases with elevation for amphibians combined and frogs globally, and in the Americas. In contrast, whereas extinction risk is greater among high-elevation Eurasian amphibians, this relationship is explained by larger body sizes and lower fecundity.Main Conclusions
Our analyses indicate that after considering factors previously thought to explain the increase in extinction risk towards higher elevations (e.g., geographic range size, disease), elevation remains a significant predictor of amphibian extinction risk. Given that the only available tests of this hypothesis in other tetrapods (birds and reptiles) conflict with our findings, we suggest that physiological or life-history features of amphibians may explain this observed phenomenon. 相似文献150.
Gold salts and phenylbutazone selectively inhibit the synthesis of PGF2α and PGE2 respectively. Lowered production of one prostaglandin species is accompanied by an increased production of the other. Selective inhibition by these drugs was observed in the presence of adrenaline, reduced glutathione and copper sulphate under conditions when most anti-inflammatory compounds inhibited PGE2 and PGF2α syntheses equally. It is postulated that selective inhibitors may have a different mode of action in vivo and beneficial effects may be related to the endogenous ratio of PGE to PGF required for normal function. 相似文献