Sex-specific activity and function of hypothalamic nonapeptide neurons during nest-building in zebra finches

Vertebrate species from fish to humans engage in a complex set of preparatory behaviors referred to as nesting; yet despite its phylogenetic ubiquity, the physiological and neural mechanisms that underlie nesting are not well known. We here test the hypothesis that nesting behavior is influenced by the vasopressin–oxytocin (VP–OT) peptides, based upon the roles they play in parental behavior in mammals. We quantified nesting behavior in male and female zebra finches following both peripheral and central administrations of OT and V_1a receptor (OTR and V1aR, respectively) antagonists. Peripheral injections of the OTR antagonist profoundly reduce nesting behavior in females, but not males, whereas comparable injections of V1aR antagonist produce relatively modest effects in both sexes. However, central antagonist infusions produce no effects on nesting, and OTR antagonist injections into the breast produce significantly weaker effects than those into the inguinal area, suggesting that antagonist effects are mediated peripherally, likely via the oviduct. Finally, immunocytochemistry was used to quantify nesting-induced Fos activation of nonapeptide neurons in the paraventricular and supraoptic nuclei of the hypothalamus and the medial bed nucleus of the stria terminalis. Nest-building induced Fos expression within paraventricular VP neurons of females but not males. Because the avian forms of OT (Ile⁸-OT; mesotocin) and VP (Ile³-VP; vasotocin) exhibit high affinity for the avian OTR, and because both peptide forms modulate uterine contractility, we hypothesize that nesting-related stimuli induce peptide release from paraventricular vasotocin neurons, which then promote female nesting via peripheral feedback from OTR binding in the oviduct uterus.     

Acid sensitivity induction (ASI) at alkaline pH in Escherichia coli involves two major sensitization components, induction of both being switched on by increased internal pH

Acid sensitivity induction (ASI) at alkaline pH in Escherichia coli 1157 ( phoE ) is only fully mounted if protein synthesis occurs for the first 5 min of the 12–15 min induction period, but appreciable sensitization occurs in the presence of chloramphenicol indicating that there are protein synthesis-independent and -dependent components (components 1 and 2). Component 1 sensitization is in place after 10 min at pH_o 9.0 but the dependent process is induced slightly more slowly. Collapsing ΔpH at pH_o 9.0 did not prevent full ASI, indicating that component 1 and 2 induction does not depend on increased ΔpH. The two induction (or activation) processes responded differently to intermediate levels of external alkalinization; component 1 was induced at pH_o 8.3 but component 2 needed pH_o 8.4 or greater. Collapsing ΔpH at pH_o 8.0 led to full induction of component 1 and appreciable induction of component 2, indicating that both processes are triggered if pH_i rises to 8.0 or greater (presumably at pH_o 8.3–8.4). Component 1 appears less important for ASI in 1157-4 ( phoE ⁺) than in 1157 ( phoE ).     

What's in a name? Considerations of homologies and nomenclature for vertebrate social behavior networks

Behavioral neuroendocrinology is an integrative discipline that spans a wide range of taxa and neural systems, and thus the appropriate designation of homology (sameness) across taxa is critical for clear communication and extrapolation of findings from one taxon to another. In the present review we address issues of homology that relate to neural circuits of social behavior and associated systems that mediate reward and aversion. We first address a variety of issues related to the so-called “social behavior network” (SBN), including homologies that are only partial (e.g., whereas the preoptic area of fish and amphibians contains the major vasopressin–oxytocin cell groups, these populations lie in the hypothalamus of other vertebrates). We also discuss recent evidence that clarifies anterior hypothalamus and periaqueductal gray homologies in birds. Finally, we discuss an expanded network model, the “social decision-making network” (SDM) which includes the mesolimbic dopamine system and other structures that provide an interface between the mesolimbic system and the SBN. This expanded model is strongly supported in mammals, based on a wide variety of evidence. However, it is not yet clear how readily the SDM can be applied as a pan-vertebrate model, given insufficient data on numerous proposed homologies and a lack of social behavior data for SDM components (beyond the SBN nodes) for amphibians, reptiles or fish. Functions of SDM components are also poorly known for birds. Nonetheless, we contend that the SDM model provides a very sound and important framework for the testing of many hypotheses in nonmammalian vertebrates.     

Cephenniini with prothoracic glands and internal cavities: new taxa,enigmatic characters and phylogeny of the Cephennomicrus group of genera (Coleoptera,Staphylinidae, Scydmaeninae)

Genera of the Cephennomicrus group of the Cephenniini (Scydmaenitae) are revised, and the following new taxa are described: Trichokrater gen.n. , Trichokrater ekkentros sp.n. (type species of Trichokrater) (Borneo), Cephennococcus gen.n. , Cephennococcus kuchingensis sp.n. (type species of Cephennococcus) (Borneo), Cephennococcus kenyirensis sp.n. (West Malaysia), Cephennococcus crassus sp.n. (Borneo), Cephennococcus minutissimus sp.n. (West Malaysia), Pomphopsilla gen.n. , Pomphopsilla luhya sp.n. (type species of Pomphopsilla) (Kenya) and Pomphopsilla soror sp.n. (Kenya). Unique subcuticular pockets with setose openings on the pronotum of Trurlia and Trichokrater are identified as glandular structures. Enigmatic internal prothoracic cavities are described for the first time in Scydmaeninae (in Cephennococcus, Pomphopsilla and a female of an undescribed genus from Sulawesi); their fine structure and function remain unknown. Parsimony‐based cladistic analysis of the adult morphology of genera of Cephenniini provided robust evidence for a monophyly of the Cephennomicrus group, composed of Cephennomicrus, Cephennula, Lathomicrus, Pomphopsilla, Cephennococcus, Trurlia, Trichokrater and two undescribed Oriental genera known from females only; this distinct and well‐supported lineage is a sister group of Cephennodes + Hlavaciellus. The genus Cephennomicrus represented in the analysis by species belonging to three previously postulated species groups is not monophyletic, and a comprehensive study comprising more taxa is necessary to reclassify this heterogeneous group.     

Infants' exploration of scented toys: effects of prior experiences

To evaluate breastfed infants' responses to scented objects, we videotaped the facial and bodily reactions of sixty-three infants as they explored, in succession, three toys that were identical in appearance but different in their characteristic odor. Two of the toys were scented with odorants previously shown to be transmitted to human milk, one with ethanol and the other with vanilla, whereas the third toy was unscented. Each videotape was subjected to frame-by-frame analysis to measure a variety of behaviors that are considered either to be exploratory in nature in that they lead to perceptual information about the object or to reflect the infants' hedonic reaction. Analyses of these behaviors revealed that the infants looked more and vocalized less in the presence of the vanilla-scented toy and spent less time manipulating the ethanol-scented toy when compared with the unscented toy. Moreover, differential exposure to the odors of ethanol and vanilla, as indicated by differential consumption of alcohol by a parent or use of vanilla-scented product by the mother, was related to differential responses to these odors. These findings suggest that human infants are able to detect and retain information about the chemical features of their environment.      

Cofilin-1: A Modulator of Anxiety in Mice

The genes involved in conferring susceptibility to anxiety remain obscure. We developed a new method to identify genes at quantitative trait loci (QTLs) in a population of heterogeneous stock mice descended from known progenitor strains. QTLs were partitioned into intervals that can be summarized by a single phylogenetic tree among progenitors and intervals tested for consistency with alleles influencing anxiety at each QTL. By searching for common Gene Ontology functions in candidate genes positioned within those intervals, we identified actin depolymerizing factors (ADFs), including cofilin-1 (Cfl1), as genes involved in regulating anxiety in mice. There was no enrichment for function in the totality of genes under each QTL, indicating the importance of phylogenetic filtering. We confirmed experimentally that forebrain-specific inactivation of Cfl1 decreased anxiety in knockout mice. Our results indicate that similarity of function of mammalian genes can be used to recognize key genetic regulators of anxiety and potentially of other emotional behaviours.