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191.
Soheila Asadi Mohammad Nabi Moradi Nejat Khyripour Mohammad Taghi Goodarzi Marzieh Mahmoodi 《Biological trace element research》2017,177(1):132-138
Diabetes is a common metabolic disorder characterized by elevated blood glucose level. Trace element homeostasis causes disturbances in diabetes due to hyperglycemia. Superoxide dismutase (SOD), an antioxidant enzyme, contains zinc and copper ions as its cofactors. Defects in SOD level and activity have been observed in diabetes. Resveratrol (RSV) has displayed hypoglycemic effects and is proven to improve oxidative stress. The aim of the present study was to examine the possible effects of RSV on blood glucose level, serum copper and zinc levels, SOD, and a number of other oxidative markers in type 2 diabetic rats. Diabetes was induced in male Wistar rats with administration of streptozotocin and nicotine amide. The studied groups containing six animals per group were as follows: group 1 normal control group; group 2 diabetic control group; groups 3, 4, and 5 diabetic rats that received 1, 5, and 10 mg/kg body weight of RSV, respectively for 30 days. Serum glucose, copper, zinc, SOD activity, total oxidant status (TOS) as well as thiol groups were all measured. Blood glucose in RSV treated groups significantly decreased. Similarly, copper significantly decreased in diabetic groups treated with RSV. Treatment with 10 mg/kg RSV resulted in significantly increased serum zinc. Furthermore, Cu/Zn ratio was observed to decrease in treated groups compared with untreated diabetic control group. RSV treated groups revealed an increased level of SOD activity as well as improved oxidative status. In summary, the results showed that RSV has potential hypoglycemic effect, attenuates trace element homeostasis, and consequently increases SOD activity level. 相似文献
192.
Albert Eduardo Silva Martins Norma Lucena-Silva Renan Gomes Garcia Stefan Welkovic Aureliana Barboza Maria Luiza Bezerra Menezes Magda Maruza Terezinha Tenório Ricardo AA Ximenes 《Memórias do Instituto Oswaldo Cruz》2014,109(6):738-747
Human immunodeficiency virus (HIV)-positive patients have a greater prevalence of
coinfection with human papillomavirus (HPV) is of high oncogenic risk. Indeed, the
presence of the virus favours intraepithelial squamous cell lesion progression and
may induce cancer. The aim of this study was to evaluate the prevalence of HPV
infection, distribution of HPV types and risk factors among HIV-positive patients.
Cervical samples from 450 HIV-positive patients were analysed with regard to oncotic
cytology, colposcopy and HPV presence and type by means of polymerase chain reaction
and sequencing. The results were analysed by comparing demographic data and data
relating to HPV and HIV infection. The prevalence of HPV was 47.5%. Among the
HPV-positive samples, 59% included viral types of high oncogenic risk. Multivariate
analysis showed an association between HPV infection and the presence of cytological
alterations (p = 0.003), age greater than or equal to 35 years (p = 0.002), number of
partners greater than three (p = 0.002), CD4+ lymphocyte count <
200/mm3 (p = 0.041) and alcohol abuse (p = 0.004). Although high-risk
HPV was present in the majority of the lesions studied, the low frequency of HPV 16
(3.3%), low occurrence of cervical lesions and preserved immunological state in most
of the HIV-positive patients were factors that may explain the low occurrence of
precancerous cervical lesions in this population. 相似文献
193.
Xiphias Ge Zhu Aleksey Chudnovskiy Lou Baudrier Benjamin Prizer Yuyang Liu Benjamin N. Ostendorf Norihiro Yamaguchi Abolfozl Arab Bernardo Tavora Rebecca Timson Søren Heissel Elisa de Stanchina Henrik Molina Gabriel D. Victora Hani Goodarzi Kıvanç Birsoy 《Cell metabolism》2021,33(1):211-221.e6
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194.
E. Bahreini R. Abbasalipourkabir M. T. Goodarzi M. Saidijam S. S. Safavieh 《Preparative biochemistry & biotechnology》2013,43(5):510-528
The gram-negative bacterium Escherichia coli (E. coli) offers a means for rapid, high-yield, and economical production of recombinant proteins. Here, a protocol for optimization of parameters involved in bacterial expression conditions is described. L-Asparaginase (ASNase II) was chosen as a model protein for our experiments. ASNase II gene (ansB) was cloned into the pAED4 plasmid and transformed into E. coli BL21pLysS (DE3)-competent cells. It was assumed that high cell density and high copy number of recombinant plasmid in the bacteria host could result in very high production of the recombinant protein. Circumstances for the overproduction of recombinant ASNase II including cell growth conditions, isopropyl β-D-1-thiogalactopyranoside (IPTG) level, ampicillin (Amp) concentration before and during IPTG induction, and cell density were optimized. Regarding the final optimization, overexpression of ASNase II was assessed on a large scale in LB medium. Periplasmic ASNase II was extracted using an alkaline lysis method. The extracted protein was purified by one-step DEAE-Sepharose fast-flow chromatography. ASNase II activity was considered an index for the protein expression. Applying the optimized practical protocol, protein production was significantly enhanced in comparison to the traditional IPTG induction method in the absence of a fermentor and can be applied for overexpression of other recombinant proteins. 相似文献
195.
Archives of Microbiology - 相似文献
196.
197.
Babak Arjmand Seyed Hamidreza Aghayan Parisa Goodarzi Mohammad Farzanehkhah Seyed Mohamadjavad Mortazavi Mohamad Hossein Niknam Ali Jafarian Farzin Arjmand Soheyla Jebelly far 《Cell and tissue banking》2009,10(3):247-252
Iranian Tissue Bank prepares a wide range of human tissue homografts such as; heart valve, bone, skin, amniotic membrane and
other tissues for different clinical applications. The purpose of this study was to determine the seroprevalence of HTLV in
tissue donors. About 1,548 tissue donors were studied during a 5-years period by ELISA assays. HTLV1,2—antibodies were tested for all of donors with other tests upon American Association of Tissue Banks (AATB) standards. About
25 (1.61%) out of 1,548 tissue donors were HTLV positive that 17 donors were male and 8 were female (female/male ratio was
approximately 47%). Regarding to the prevalence of HTLV among tissue donors and importance of cell and tissue safety and quality
assurance, we recommend that all blood, cell and tissue banks should be involved both routine serological methods and other
complementary tests such as polymerase chain reaction (PCR) for diagnosis of HTLV. 相似文献
198.
Feriyar Khoshayand Sanaz Goodarzi Ahmad Reza Shahverdi Mohammad Reza Khoshayand 《Probiotics and antimicrobial proteins》2011,3(3-4):159-167
Soymilk was fermented with Lactobacillus casei, and statistical experimental design was used to investigate factors affecting viable cells of L. casei, including temperature, glucose, niacin, riboflavin, pyridoxine, folic acid and pantothenic acid. Initial screening by Plackett-Burman design revealed that among these factors, temperature, glucose and niacin have significant effects on the growth of L. casei. Further optimization with Box-Behnken design and response surface analysis showed that a second-order polynomial model fits the experimental data appropriately. The optimum conditions for temperature, glucose and niacin were found to be 15.77 °C, 5.23 and 0.63 g/L, respectively. The concentration of viable L. casei cells under these conditions was 8.23 log10 (CFU/mL). The perfect agreement between the observed values and the values predicted by the equation confirms the statistical significance of the model and the model’s adequate precision in predicting optimum conditions. 相似文献
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