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931.
内皮祖细胞(EPC)是一种多潜能细胞,主要来源于骨髓。外周血EPC可以参与修复多种血管内皮细胞损伤的疾病。目前研究证实EPC通过动员、迁移、归巢和分化等步骤在受损的肺组织处参与内皮细胞修复,调节失控的炎症反应,增强抗氧化能力,对修复和维持肺泡毛细血管屏障的完整性起着重要作用。EPC在心血管疾病和组织工程领域应用研究的成功,为EPC在急性肺损伤的治疗提供了新的思路。 相似文献
932.
LI Ning XU Zhong Wei ZHAI Lin Hui LI Yan Chang FAN Feng Xu ZHENG Jun Jie XU Ping HE Fu Chu 《中国科学:生命科学英文版》2014,(12)
Proteomics focuses on the systematic identification and quantification of entire proteomes and interpretation of proteins’biological functions.During the last decade,proteomics in China has grown much faster than other research fields in life sciences.At the beginning of the second decade of the 21st century,the rapid development of high-resolution and high-speed mass spectrometry makes proteomics a powerful tool to study the mechanisms underlying physiological/pathological processes in organisms.This article provides a brief overview of proteomics technology development and representative scientific progress of the Human Liver Proteome Project in China over the past three years. 相似文献
933.
Yung-Chi Chang Joshua Olson Federico C. Beasley Christine Tung Jiquan Zhang Paul R. Crocker Ajit Varki Victor Nizet 《PLoS pathogens》2014,10(1)
Group B Streptococcus (GBS) is a common agent of bacterial sepsis and meningitis in newborns. The GBS surface capsule contains sialic acids (Sia) that engage Sia-binding immunoglobulin-like lectins (Siglecs) on leukocytes. Here we use mice lacking Siglec-E, an inhibitory Siglec of myelomonocytic cells, to study the significance of GBS Siglec engagement during in vivo infection. We found GBS bound to Siglec-E in a Sia-specific fashion to blunt NF-κB and MAPK activation. As a consequence, Siglec-E-deficient macrophages had enhanced pro-inflammatory cytokine secretion, phagocytosis and bactericidal activity against the pathogen. Following pulmonary or low-dose intravenous GBS challenge, Siglec-E KO mice produced more pro-inflammatory cytokines and exhibited reduced GBS invasion of the central nervous system. In contrast, upon high dose lethal challenges, cytokine storm in Siglec-E KO mice was associated with accelerated mortality. We conclude that GBS Sia mimicry influences host innate immune and inflammatory responses in vivo through engagement of an inhibitory Siglec, with the ultimate outcome of the host response varying depending upon the site, stage and magnitude of infection. 相似文献
934.
Dongmei Ji Zhe Zhang Lei Cheng Jinjia Chang Shanshan Wang Biqiang Zheng Rongliang Zheng Zuojun Sun Chenchen Wang Zhiqing Zhang Rujiao Liu Xiaowei Zhang Xin Liu Xiaofeng Wang Jin Li 《PloS one》2014,9(1)
In the current study, we showed that the combination of mammalian target of rapamycin (mTOR) inhibitor RAD001 (everolimus) and Akt inhibitor MK-2206 exerted synergistic cytotoxic effects against low-phosphatase and tensin homolog (PTEN) gastric cancer cells (HGC-27 and SNU-601 lines). In HGC-27 cells, RAD001 and MK-2206 synergistically induced G1/S cell cycle arrest, growth inhibition, cell death but not apoptosis. RAD001 and MK-2206 synergistically induced light chain 3B (LC3B) and beclin-1 expression, two important autophagy indicators. Meanwhile, the autophagy inhibitor 3-methyladenine (3-MA) and chloroquine inhibited the cytotoxic effects by RAD001 and MK-2206, suggesting that autophagic, but not apoptotic cell death was important for the cytotoxic effects by the co-administration. We observed that the combination of RAD001 and MK-2206 exerted enhanced effects on Akt/mTOR inhibition, cyclin D1 down-regulation and ERK/MAPK(extracellular signal-regulated kinase/mitogen-activated protein kinases) activation. Intriguingly, MEK/ERK inhibitors PD98059 and U0126 suppressed RAD001 plus MK-2206-induced beclin-1 expression, autophagy induction and cytotoxicity in HGC-27 cells. In conclusion, these results suggested that the synergistic anti-gastric cancer cells ability by RAD001 and MK-2206 involves ERK-dependent autophagic cell death pathway. 相似文献
935.
基于黑龙江省2010年一类调查数据和重点公益林检测样地(5075块)数据以及同期黑龙江省、吉林省和内蒙古自治区59个气象站的气象数据,以森林碳储量为因变量,以胸径、每公顷株数、海拔、坡度及降雨与温度的乘积因子作为自变量,利用GeoDA软件构建空间误差模型,用全局Moran I 来描述不同空间尺度下模型残差的空间自相关性,计算最佳带宽(25 km)下的局域Moran I来表现模型残差的空间分布,计算组内方差来解释模型残差的空间异质性,最后将模型的预估结果生成黑龙江省森林碳储量的空间分布图.结果表明: 黑龙江省森林碳储量的分布具有空间效应;本文所选林分因子、地形因子及气象因子都显著影响森林碳储量的空间分布,胸径是最主要的因子.空间误差模型可以很好地解决模型残差的空间自相关性及空间异质性.由模型的预估结果可以看出,森林碳储量的空间分布存在很大差异,张广才岭、小兴安岭及大兴安岭地区是森林分布较密集的区域,松嫩平原地区的森林碳储量分布较少,完达山地区处于中等水平.
相似文献
936.
Jianqiang Li Zhengwang Zhang Lei Lv Chang Gao Yong Wang 《Ethology : formerly Zeitschrift fur Tierpsychologie》2014,120(2):159-168
When benefits exceed costs, natural selection may favor adults that develop the ability to recognize and preferentially direct care toward their own offspring to maximize their fitness. Investigations into the ability of adults to recognize offspring in offspring's early development period may help to understand when the ability of kin recognition starts to develop. In birds, studies of offspring recognition have mainly been conducted on bi‐parental breeding species, but relatively seldom on cooperative breeding species, despite that kin recognition may be of particular importance for cooperative breeders. The silver‐throated tit Aegithalos glaucogularis is a small passerine in which some nests have helpers during breeding. We tested whether silver‐throated tit parents and helpers were able to distinguish between their own and alien nestlings 2–5 d before fledging when recognition mechanisms were likely to have been developed. Through two forced choice experiments, of which one was conducted right beside the experimental nests (<0.8 m) and the other far away from the experimental nests (~6 m), we found that neither parents nor helpers discriminatively fed their own and alien nestlings, which suggested that at least during the experimental nestling age, and within the 6‐m‐radius area around the nests, they might not have the ability to recognize offspring. The possibility that silver‐throated tits use a larger area (>6 m radius) around their nests as a location‐based cue for offspring recognition, or would develop an offspring recognition ability at an older nestling age and/or even after fledging, warrants future studies. 相似文献
937.
Development of a Novel Selective and Differential Medium for the Isolation of Listeria monocytogenes
Sang-Hyun Park Pahn-Shick Chang Sangryeol Ryu Dong-Hyun Kang 《Applied and environmental microbiology》2014,80(3):1020-1025
A new medium (lecithin and levofloxacin [LL] medium) is described for the isolation of Listeria monocytogenes from food samples. LL medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase C (PI-PLC) and phosphatidylcholine-specific phospholipase C (PC-PLC) produced by L. monocytogenes. Levofloxacin is incorporated to inhibit the growth of microorganisms other than L. monocytogenes, especially Bacillus cereus, shown to possess PI-PLC and PC-PLC activities. L. monocyogenes produced white colonies with a halo on LL medium, whereas Listeria innocua appeared as white colonies without a halo. Levofloxacin at 0.20 mg/liter completely inhibited the growth of B. cereus, while the growth of L. monocytogenes was unaffected. In the second phase of the study, the sensitivity and the specificity of LL medium were compared to those of modified Oxford agar (MOX) and two chromogenic media (Brilliance Listeria agar and CHROMagar Listeria), using a total of 250 food samples. From 200 unspiked food samples, the specificity of LL medium (96.0%) was superior to that of MOX (72.0%) and similar to the specificities of Brilliance Listeria agar (96.5%) and CHROMagar Listeria (94.5%). From 50 spiked food samples, LL medium and CHROMagar Listeria represented the highest sensitivities (96.0%), followed by Brilliance Listeria agar (92.0%) and MOX (54.0%). Also, LL medium showed the highest confirmation rate (98.8%), followed by Brilliance Listeria agar (98.7%), CHROMagar Listeria (98.3%), and MOX (52.0%). On the basis of its good specificity and cost effectiveness, LL medium is useful for the isolation of L. monocytogenes from food samples. 相似文献
938.
939.
Radiation pressure on a biconcave human Red Blood Cell and the resulting deformation in a pair of parallel optical traps 下载免费PDF全文
Guan‐Bo Liao Yin‐Quan Chen Paul B. Bareil Yunlong Sheng Arthur Chiou Ming‐Shien Chang 《Journal of biophotonics》2014,7(10):782-787
We calculated the three‐dimensional optical stress distribution and the resulting deformation on a biconcave human red blood cell (RBC) in a pair of parallel optical trap. We assumed a Gaussian intensity distribution with a spherical wavefront for each trapping beam and calculated the optical stress from the momentum transfer associated with the reflection and refraction of the incident photons at each interface. The RBC was modelled as a biconcave thin elastic membrane with uniform elasticity and a uniform thickness of 0.25 μm. The resulting cell deformation was determined from the optical stress distribution by finite element software, Comsol Structure Mechanics Module, with Young's modulus (E) as a fitting parameter in order to fit the theoretical results for cell elongation to our experimental data. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
940.
Boyin Jia Zhiguang Chang Yana Zhang Xiaoyan Wei Jianhua Zhou Henan Wang Xin Zhao Shengchao Yu Meng Song Zhiwei Tu Huijun Lu Jigang Yin Mats Wahlgren Qijun Chen 《Proteomics》2014,14(15):1737-1745
Toxoplasma gondii is an obligatory intracellular apicomplexan parasite which exploits host cell surface components in cell invasion and intracellular parasitization. Sulfated glycans such as heparin and heparan sulfate have been reported to inhibit cell invasion by T. gondii and other apicomplexan parasites such as Plasmodium falciparum. The aim of this study was to investigate the heparin‐binding proteome of T. gondii. The parasite‐derived components were affinity‐purified on the heparin moiety followed by MS fingerprinting of the proteins. The heparin‐binding proteins of T. gondii and P. falciparum were compared based on functionality and affinity to heparin. Among the proteins identified, the invasion‐related parasite ligands derived from tachyzoite/merozoite surface and the secretory organelles were prominent. However, the profiles of the proteins were different in terms of affinity to heparin. In T. gondii, the proteins with highest affinity to heparin were the intracellular components with functions of parasite development contrasted to that of P. falciparum, of which the rhoptry‐derived proteins were prominently identified. The profiling of the heparin‐binding proteins of the two apicomplexan parasites not only explained the mechanism of heparin‐mediated host cell invasion inhibition, but also, to a certain extent, revealed that the action of heparin on the parasite extended after endocytosis. 相似文献