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31.
32.
Gigantism in isolated ponds in the absence of sympatric fish species has previously been observed in nine-spined sticklebacks
(Pungitius pungitius). Patterns in sexual size dimorphism suggested that fecundity selection acting on females might be responsible for the phenomenon.
However, the growth strategy behind gigantism in pond sticklebacks has not been studied yet. Here, we compared von Bertalanffy
growth parameters of four independent nine-spined stickleback populations reared in a common laboratory environment: two coastal
marine (typical size) and two pond (giant size) populations. We found that both pond populations had larger estimated final
size than marine populations, which in turn exhibited higher intrinsic growth rates than the pond populations. Female growth
strategies were more divergent among marine and pond populations than those of males. Asymptotic body size and intrinsic growth
rate were strongly negatively correlated. Hence, pond versus marine populations exhibited different growth strategies along
a continuum. Our data suggest that quick maturation—even with the cost of being small (low fecundity)—is favoured in marine
environments. On the contrary, growth to a giant final size (high fecundity)—even if it entails extended growth period—is
favoured in ponds. We suggest that the absence (ponds) versus presence (marine environment) of sympatric predatory fish species,
and the consequent change in the importance of intraspecific competition are responsible for the divergence in growth strategies.
The sex-dependence of the patterns further emphasizes the role of females in the body size divergence in the species. Possible
alternative hypotheses are also discussed. 相似文献
33.
34.
Although the brain is known to be a very plastic organ, the effects of common ecological interactions like predation or competition on brain development have remained largely unexplored. We reared nine-spined sticklebacks (Pungitius pungitius) from two coastal marine (predation-adapted) and two isolated pond (competition-adapted) populations in a factorial experiment, manipulating perceived predatory risk and food supply to see (i) if the treatments affected brain development and (ii) if there was population differentiation in the response to treatments. We detected differences in plasticity of the bulbus olfactorius (chemosensory centre) between habitats: marine fish were not plastic, whereas pond fish had larger bulbi olfactorii in the presence of perceived predation. Marine fish had larger bulbus olfactorius overall. Irrespective of population origin, the hypothalamus was smaller in the presence of perceived predatory risk. Our results demonstrate that perceived predation risk can influence brain development, and that the effect of an environmental factor on brain development may depend on the evolutionary history of a given population in respect to this environmental factor. 相似文献
35.
Katakai T Suto H Sugai M Gonda H Togawa A Suematsu S Ebisuno Y Katagiri K Kinashi T Shimizu A 《Journal of immunology (Baltimore, Md. : 1950)》2008,181(9):6189-6200
Mesenchymal stromal cells are crucial components of secondary lymphoid organs (SLOs). Organogenesis of SLOs involves specialized stromal cells, designated lymphoid tissue organizer (LTo) in the embryonic anlagen; in the adult, several distinct stromal lineages construct elaborate tissue architecture and regulate lymphocyte compartmentalization. The relationship between the LTo and adult stromal cells, however, remains unclear, as does the precise number of stromal cell types that constitute mature SLOs are unclear. From mouse lymph nodes, we established a VCAM-1(+)ICAM-1(+)MAdCAM-1(+) reticular cell line that can produce CXCL13 upon LTbetaR stimulation and support primary B cell adhesion and migration in vitro. A similar stromal population sharing many characteristics with the LTo, designated marginal reticular cells (MRCs), was found in the outer follicular region immediately underneath the subcapsular sinus of lymph nodes. Moreover, MRCs were commonly observed at particular sites in various SLOs even in Rag2(-/-) mice, but were not found in ectopic lymphoid tissues, suggesting that MRCs are a developmentally determined element. These findings lead to a comprehensive view of the stromal composition and architecture of SLOs. 相似文献
36.
We developed a new method for imaging the movement of targeted proteins in living cancer cells with photostable and bright quantum dots (QDs). QDs were conjugated with various molecules and proteins, such as phalloidin, anti-tubulin antibody and kinesin. These bioconjugated QDs were mixed with a transfection reagent and successfully internalized into living cells. The movements of individual QDs were tracked for long periods of time. Phalloidin conjugated QDs bound to actin filaments and showed almost no movement. In contrast, anti-tubulin antibody conjugated QDs bound to microtubules and revealed dynamic movement of microtubules. Kinesin showed an interesting behavior whereby kinesin came to be almost paused briefly for a few seconds and then moved once again. This is in direct contrast to the smoothly continuous movement of kinesin in an in vitro assay. The maximum velocity of kinesin in cells was faster than that in the in vitro assay. These results suggest that intracellular movement of kinesin is different from that in the in vitro assay. This newly described method will be a powerful tool for investigating the functions of proteins in living cells. 相似文献
37.
Subcellular localization of the Schlafen protein family 总被引:1,自引:0,他引:1
Neumann B Zhao L Murphy K Gonda TJ 《Biochemical and biophysical research communications》2008,370(1):62-66
Although the first members of the Schlafen gene family were first described almost 10 years ago, the precise molecular/biochemical functions of the proteins they encode still remain largely unknown. Roles in cell growth, haematopoietic cell differentiation, and T cell development/maturation have, with some experimental support, been postulated, but none have been conclusively verified. Here, we have determined the subcellular localization of Schlafens 1, 2, 4, 5, 8, and 9, representing all three of the murine subgroups. We show that the proteins from subgroups I and II localize to the cytoplasm, while the longer forms in subgroup III localize exclusively to the nuclear compartment. We also demonstrate upregulation of Schlafen2 upon differentiation of haematopoietic cells and show this endogenous protein localizes to the cytoplasm. Thus, we propose the different subgroups of Schlafen proteins are likely to have functionally distinct roles, reflecting their differing localizations within the cell. 相似文献
38.
M Yanabe M Shibuya T Gonda H Asai T Tanaka K Sudou T Narita T Matsui K Itoh 《Experimental Animals》2001,50(2):105-113
Six groups of limited flora (LF) Hartley guinea-pigs were produced by inoculation of hysterectomy-derived GF guinea-pigs with various combinations of cecal bacteria of conventional (CV) guinea-pigs to determine the effective bacterial cocktails for the establishment of a specific pathogen free (SPF) colony. Bifidobacterium magnum (Bif) isolated from CV guinea-pigs was used for pretreatment. The mortality of LF guinea-pigs inoculated with only Bif was 75%, and that of those inoculated with Bif plus chloroform-treated cecal suspension (CHF) or Bif plus CHF plus 32 isolates from CV guinea-pigs was 40 to 66.7%. These three groups were in an unhealthy condition with mucoid enteritis-like diarrhea. However, the mortality of LF guinea-pigs inoculated with the anaerobic growth on EG plates injected with 10(-5) dilution of cecal contents (CF) or inoculated with Bif plus CF was 6.3 and 15%, respectively. These latter two groups of LF guinea-pigs were transferred to separate barrier rooms and some of the LF guinea-pigs were maintained in isolators as a source of intestinal flora for SPF guinea-pigs. The composition of cecal flora of LF guinea-pigs was stable for a long time, and bacteroidaceae and peptococcaceae were maintained as predominant components. The basic composition of the cecal flora of SPF guinea-pigs originated from LF guinea-pigs, which consists mainly of the anaerobic bacteria, was not changed over a long period, and the flora composition became similar to that in CV guinea-pigs. Guinea-pig-specific pathogens from the SPF colonies were not detected during experiments. 相似文献
39.
By searching processively RecA protein pairs DNA molecules that share a limited stretch of homology 总被引:1,自引:0,他引:1
RecA protein promotes homologous pairing by a reaction in which the protein first binds stoichiometrically to single-stranded DNA in a slow presyn-aptic step, and then conjoins single-stranded and duplex DNA, thereby forming a ternary complex. RecA protein did not pair molecules that shared only 30 bp homology, but, with full efficiency, it paired circular single-stranded and linear duplex molecules in which homology was limited to 151 bp at one end of the duplex DNA. The initial rate of the pairing reaction was directly related to the length of the heterologous part of the duplex DNA, which we varied from 0 to 3060 base pairs. Since interactions involving the heterologous part of a molecule speed the location of a small homologous region, we conclude that RecA protein promotes homologous alignment by a processive mechanism involving relative motion of conjoined molecules within the ternary complex. 相似文献
40.
This article suggests how we might understand the way potential predators see coloration patterns used in aposematism and
visual mimicry. We start by briefly reviewing work on evolutionary function of eyes and neural mechanisms of vision. Often
mechanisms used for achromatic vision are accurately modeled as adaptations for detection and recognition of the generality
of optical stimuli, rather than specific stimuli such as biological signals. Colour vision is less well understood, but for
photoreceptor spectral sensitivities of birds and hymenopterans there is no evidence for adaptations to species-specific stimuli,
such as those of food or mates. Turning to experimental work, we investigate how achromatic and chromatic stimuli are used
for object recognition by foraging domestic chicks (Gallus gallus). Chicks use chromatic and achromatic signals in different ways: discrimination of large targets uses (chromatic) colour
differences, and chicks remember chromatic signals accurately. However, detection of small targets, and discrimination of
visual textures requires achromatic contrast. The different roles of chromatic and achromatic information probably reflect
their utility for object recognition in nature. Achromatic (intensity) variation exceeds chromatic variation, and hence is
more informative about change in reflectance – for example, object borders, while chromatic signals yield more information about surface reflectance (object
colour) under variable illumination.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献