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101.
E N Goncharenko T G Gorskaia T I Gudz' A V Gurovich L T Zolotareva 《Radiobiologiia》1978,18(2):268-271
102.
M. M. Moisenovich D. A. Kulikov A. V. Goncharenko A. Y. Arkhipova T. V. Vasiljeva Yu. N. Filyushkin L. V. Arkhipova M. S. Kotlyarova A. V. Kulikov A. E. Mashkov I. I. Agapov M. P. Kirpichnikov 《Doklady. Biochemistry and biophysics》2017,472(1):12-14
Regenerative properties of fibroin implant vitalized with allogeneic bone marrow cells were assessed. The study was performed using the experimental model of rat jejunum wall damage. Three weeks after surgery, we observed recovery of all layers of the jejunum wall at the site of injury and complete degradation of the implant material. 相似文献
103.
E I Vasil'eva E N Goncharenko T I Gudz' I I Samo?lenko 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1984,(9):30-33
Superoxide dismutase and catalase activity has been studied in isogenous strains of various radioresistance bacteria. In mutants Micrococcus radiodurans having defects in the systems of DNA repair the superoxide dismutase activity is lower than in cells of wild type. The changes of catalase and superoxide dismutase activity have not been revealed in investigated strains Escherichia coli differing in radioresistance. It has been concluded that the survival of bacteria exposed to ionizing radiation is determined by the effectiveness of DNA repair systems realiability of which depends on the catalase and superoxide dismutase activity. 相似文献
104.
V. I. Tishkov K. V. Goncharenko A. A. Alekseeva S. Yu. Kleymenov S. S. Savin 《Biochemistry. Biokhimii?a》2015,80(13):1690-1700
Comparison of amino acid sequences of NAD+-dependent formate dehydrogenases (FDH, EC 1.2.1.2) from different sources and analysis of structures of holo-forms of FDH from bacterium Pseudomonas sp. 101 (PseFDH) and soya Glycine max (SoyFDH) as well as of structure of apo-form of FDH from yeast Candida boidinii (CboFDH) revealed the presence on the surface of protein globule of hydrophobic Phe residue in structurally equivalent position (SEP). The residue is placed in the coenzyme-binding domain and protects bound NAD+ from solvent. The effects of amino acid changes of the SEP on catalytic properties and thermal stability of PseFDH, SoyFDH, and CboFDH were compared. The strongest effect was observed for SoyFDH. All eight amino acid replacements resulted in increase in thermal stability, and in seven cases, increase in catalytic constant was achieved. Thermal stability of SoyFDH after mutations Phe290Asp and Phe290Glu increased 66- and 55-fold, respectively. K M values of the enzyme for substrate and coenzyme in different cases slightly increased or decreased. In case of one CboFDH, the mutein catalytic constant increased and thermal stability did not changed. In case of the second CboFDH mutant, results were the opposite. In one PseFDH mutant, amino acid change did not influence the catalytic constant, but in three others, the parameter was reduced. Two PseFDH mutants had higher and two mutants lower thermal stability in comparison with initial enzyme. Analysis of results of SEP mutagenesis in FDHs from bacterium, yeast, and plant shows that protein structure plays a key role for effect of the same amino acid changes in equivalent position in protein globule of formate dehydrogenases from different sources. 相似文献
105.
M. S. Kotliarova V. A. Zhuikov Y. V. Chudinova D. D. Khaidapova A. M. Moisenovich A. S. Kon’kov L. A. Safonova M. M. Bobrova A. Y. Arkhipova A. V. Goncharenko K. V. Shaitan 《Moscow University Biological Sciences Bulletin》2016,71(4):212-217
We have developed microcarriers made from silk fibroin. Microcarriers can be used as a substrate for cell cultivation and cell delivery during cell-based therapy and for the construction of bioengineered tissue. Fibroin microcarriers were mineralized, which led to the appearance of calcium phosphate crystals on their surface. The ability of mineralized and nonmineralized microcarriers to support osteogenic differentiation of the osteoblast-like cell line MG-63 was estimated by alkaline phosphatase activity, an early marker of bone formation. The experiment showed cells actively proliferating on the surface of both mineralized and nonmodified microcarriers. Culturing MG-63 on the surface of fibroin microcarriers resulted in an increase of alkaline phosphatase activity indicative of osteogenic differentiation of MG-63 cells in the absence of inductors. The level of alkaline phosphatase was higher when mineralized microcarriers were used. Alkaline phosphatase activity of MG-63 cells cultivated using traditional two-dimensional approaches were close to zero. As opposed to conventional monolayer culturing, microcarrier culture cells are in a three-dimensional environment that is closer to physiological conditions. This can have a significant impact on their morphology and functional properties. During this study, we also characterized mechanical properties of porous scaffolds used for microcarriers. 相似文献
106.
M. M. Moisenovich N. V. Malyuchenko A. Yu. Arkhipova A. V. Goncharenko M. S. Kotlyarova L. I. Davydova T. V. Vasil’eva V. G. Bogush I. I. Agapov V. G. Debabov M. P. Kirpichnikov 《Doklady. Biochemistry and biophysics》2016,466(1):9-12
The study of the stimulating effect of the microgels (MGs) based on recombinant 1F9 spidroin on the regeneration of the deep skin wound in mice was carried out. The use of spidroin MGs was shown to increase significantly the quality of healing compared to the control. The introduction of the MG in the wound edges led to recovery of all the structural elements of the skin: the epidermis, the dermis, including vascular and nervous network, in the periphery of the wound underlying muscles, and skin appendages (sebaceous and sweat glands and hair follicles) was revealed. 相似文献
107.
Background
Efficient and accurate prediction of protein function from sequence is one of the standing problems in Biology. The generalised use of sequence alignments for inferring function promotes the propagation of errors, and there are limits to its applicability. Several machine learning methods have been applied to predict protein function, but they lose much of the information encoded by protein sequences because they need to transform them to obtain data of fixed length. 相似文献108.
109.
V N Ma?suradze A G Platonov T I Gudz' E N Goncharenko Iu B Kudriashov 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1987,(5):31-35
The increase of the content of lipoperoxidation products, the decrease of antiradical activity of lipids and the activities of superoxide dismutase, glutathione peroxidase and glutathione reductase in the skin of rats have been found to be a result of ultraviolet (UV) radiation. The dose and time dependences of changes of these parameters have been obtained. It is suggested that intensification of lipid peroxidation in the skin of UV-radiated animals is correlated with the inhibition of the antiperoxide systems. 相似文献
110.