全文获取类型
收费全文 | 204篇 |
免费 | 8篇 |
专业分类
212篇 |
出版年
2021年 | 2篇 |
2020年 | 2篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 5篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 13篇 |
2005年 | 12篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 6篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 5篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 6篇 |
1982年 | 1篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1968年 | 2篇 |
1967年 | 5篇 |
1964年 | 1篇 |
排序方式: 共有212条查询结果,搜索用时 0 毫秒
11.
Maureen Craig Adam Y. Sadik Yekaterina A. Golubeva Avital Tidhar James M. Slauch 《Molecular microbiology》2013,89(5):887-902
The twin‐arginine translocation system (Tat) transports folded proteins across the cytoplasmic membrane and is critical to virulence in Salmonella and other pathogens. Experimental and bioinformatic data indicate that 30 proteins are exported via Tat in Salmonella Typhimurium. However, there are no data linking specific Tat substrates with virulence. We inactivated every Tat‐exported protein and determined the virulence phenotype of mutant strains. Although a tat mutant is highly attenuated, no single Tat‐exported substrate accounts for this virulence phenotype. Rather, the attenuation is due primarily to envelope defects caused by failure to translocate three Tat substrates, the N‐acetylmuramoyl‐l ‐alanine amidases, AmiA and AmiC, and the cell division protein, SufI. Strikingly, neither the amiA amiC nor the sufI mutations alone conferred any virulence defect. Although AmiC and SufI have previously been localized to the divisome, the synthetic phenotypes observed are the first to suggest functional overlap. Many Tat substrates are involved in anaerobic respiration, but we show that a mutant completely deficient in anaerobic respiration retains full virulence in both the oral and systemic phases of infection. Similarly, an obligately aerobic mutant is fully virulent. These results suggest that in the classic mouse model of infection, S. Typhimurium is replicating only in aerobic environments. 相似文献
12.
Tatiana V. Burova Irina A. Golubeva Natalia V. Grinberg Alexander Ya. Mashkevich Valery Ya. Grinberg Anatoly I. Usov Luciano Navarini Attilio Cesro 《Biopolymers》1996,39(4):517-529
Thermally induced order-disorder conformational transition in succinoglycan was studied using the method of high-sensitivity differential scanning microcalorimetry within the range of polysaccharide concentrations from 0.1 to 3.5 mg mL−1 at NaCl concentrations 0, 0.01, and 0.1M. The positions and shapes of the excess heat capacity curves depended substantially on both the NaCl and polysaccharide concentrations. At low polysaccharide concentrations in salt-free solution the experimental curves were closely approximated by the two-state model suggesting the transition mechanism to be of the single helix-coil type. With increasing polysaccharide and/or NaCl concentration, the experimental curves changed significantly in symmetry, which indicated a changing transition mechanism. At high polysaccharide concentrations or in the presence of the salt, the order-disorder transition of succinoglycan was shown to include two stages: the cooperative dissociation of the helix dimer and subsequent two-state melting of the helix monomer. The dependence of thermodynamic parameters for the dissociation and melting of helix structures in succinoglycan on NaCl and polysaccharide concentrations was obtained by fitting the experimental excess heat capacity curves. The cooperativity parameter σ for the single helix-coil transition as well as the average length of the helix segment of succinoglycan were calculated. Some features of succinoglycan ordering in solution are discussed. © 1996 John Wiley & Sons, Inc. 相似文献
13.
14.
Mikhailov SN Efimtseva EV Ermolinsky BS Bobkov GV Andreeva OI Golubeva AS Kochetkov SN Van Aerschot A Schepers G Herdewijn P 《Nucleosides, nucleotides & nucleic acids》2003,22(5-8):1117-1118
The efficient synthesis of oligonucleotides containing 2'-O-beta-D-ribofuranosyl (and beta-D-ribopyranosyl)nucleosides, 2'-O-alpha-D-arabinofuranosyl (and alpha-L-arabinofuranosyl)nucleosides. 2'-O-beta-D-erythrofuranosylnucleosides, and 2'-O-(5'-amino-5-deoxy-beta-D-ribofuranosyl)nucleosides have been developed. 相似文献
15.
Korneeva EV Aleksandrov LI Golubeva TB Shuleĭkina KV 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2000,50(3):518-526
Golgi-stained preparations were processed with computerized morphometry to study the effect of the deprivation (eyes covered with nontransparent caps) on the development of neurons in the Wulst (the structure analogous to mammalian visual cortex) of pied flycatcher nestlings. Six-day-old nestlings that have only one form of the visual perception--diffuse photosensitivity--were studied. In the previous paper [Korneeva et al., 1994] in was shown that the Wulst of 6-day-old nestlings consisted of non-differentiated neurons and stellate-like cells at different stages of maturation; the latter group was subdivided into more- and less-mature cells. This work proved that even a 1.5-day-long deprivation (actual duration being counted off from the moment of appearance of the first retinal photoreceptors at the age of 4.5 days) resulted in significant changes in the geometry of stellate-like neurons. The changes in less-mature cells were predominantly destructive (decline of all quantitative indices of a cell, including the significant decrease in the total length of dendrites, maximal radius of the dendritic field and cell branching index), while the changes in more-mature cells were constructive (increase in all quantitative cell indices, including statistically significant increase in the soma section area, total length of dendrites, maximal radius of dendritic field and the number of foci of maximal branching). Different reactions of these cell types to the limitation of visual afferentation may be connected with differences in the afferent inflow to less- and more-mature cells or/and with different maturational stages of these neurons at the onset of deprivation. 相似文献
16.
P. MALLE M. VALLÉ P. DEMARQUE P. EB R. TAILLIEZ 《Journal of Rapid Methods and Automation in Microbiology》1998,6(2):93-102
H2 S+ bacteria responsible for the degradation of sulfur-containing amino acids of fish muscle are currently little used to evaluate the microbiological pal quality of fish. Shewanella putrefaciens greatly predominates in this flora, and was therefore used to define a suitable culture method and medium. Inoculations by the Spiral surface method at 25C, with an incubation of 72h, gave the best counts on a medium containing two sources of sulfur (organic and inorganic) for H2 S+ bacteria. The culture medium and the NaCl concentration were determinant in the evaluation of this flora. At present there is no standard medium which meets these requirements. 相似文献
17.
R N Khodanova L S Seslavina N N Golubeva V A Zuyeva O N Kutkova V V Maltseva T I Nikitina 《Journal of hygiene, epidemiology, microbiology, and immunology》1989,33(4):463-469
An efficient method for the treatment of drug allergy was proposed and practically implemented. The method consists in autologous venous blood being lyzed with sterile bidistilled water at a ratio of 5:1 and injected subcutaneously and partially intradermally into the reflexogenic zones of the back 2-3 cm from the spinal column. During the first week of treatment, increasing doses were injected (3 to 10 ml), whereas during the second week the doses decreased from 10 to 3 ml. Following treatment the patients felt better and featured enhanced working ability as well as markedly declined susceptibility to common colds. The most informative immunological indicators were chosen to evaluate the efficiency of therapy. 相似文献
18.
Golubeva EK Nazarov SB 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2007,93(3):283-288
Influence of the polycythaemia on peritoneal and splenic macrophage function and NO production was studied. Interaction of peritoneal and splenic macrophages with autologous erythrocytes was examined. Concentration of nitrate ions (stable metabolites ofnitric oxide) was measured in peritoneal lavage, splenic cells suspension, blood, as well as in supernatant of peritoneal and splenic macrophages. The data obtained suggest that splenic macrophages from intact rats are more active than the peritoneal ones. Splenic macrophage activity decreases whereas that of peritoneal macrophages stays unaltered in polycythaemia. Concentration of nitrate ions is significantly increased in this condition. 相似文献
19.
20.
Roberto?H?Higa Roberto?C?Togawa Arnaldo?J?Montagner Juliana?CF?Palandrani Igor?KS?Okimoto Paula?R?Kuser Michel?EB?Yamagishi Adauto?L?Mancini Goran?NeshichEmail author 《BMC bioinformatics》2004,5(1):107