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31.
A Rhodotorula colostri strain has been isolated that shows fungicidal activity against related species of the genera Rhodosporidium, Sporidiobolus, and their anamorphs phylogenetically belonging to the order Sporidiobolales (Microbotryomycetes, Pucciniomycotina). The agent secreted has a molecular weight of about 4–5 kDa, is active at pH values ranging from 3.5 to 5.5, and is thermolabile and protease-sensitive. 相似文献
32.
E. V. Dubrovskaya N. N. Pozdnyakova S. N. Golubev V. S. Grinev O. V. Turkovskaya 《Russian Journal of Plant Physiology》2017,64(2):174-183
From the roots and root exudates of 3-week-old plants of alfalfa (Medicago sativa L.), anionic and cationic peroxidases differing in principal physicochemical and catalytic properties were isolated and purified. Main features of anionic peroxidases detected in the roots and root exudates were identical. Phenanthrene present in the soil used for alfalfa growing influenced the number of forms and activity of peroxidases in crude enzyme preparations but did not affect the properties of pure enzymes. In the presence of a synthetic mediator, purified peroxidases can oxidize phenanthrene and its derivatives, including potential microbial metabolites of polycyclic aromatic hydrocarbons (PAH). The fact that the enzymes excreted in root exudates in a purified form can oxidase PAH proves their participation in degradation of PAH and their microbial metabolites in alfalfa rhizosphere. These new data indicate that the processes of plant and microbial degradation of pollutants in the rhizosphere are coupled; they are relevant to understanding the molecular mechanisms of degradation of persistent pollutants by plant-microbial complexes. 相似文献
33.
34.
V. I. Golubev 《Microbiology》2000,69(4):490-493
D-Glucuronate-containing agar is suggested for evaluating the population density and diversity of tremelloid yeasts in natural
cenoses. This medium is superior to the commonly used wort agar on which many representatives of tremelloid yeasts cannot
be revealed. 相似文献
35.
A new species of the genus Cryptococcus, Cr. mycelialis (the type strain VKM Y-2863), is described based on the taxonomic study of four strains isolated from soil and plant samples collected on the South Georgia and East Falkland islands. This species differs from the known Cryptococcus species in its ability to form a true monokaryotic mycelium with pseudoclamp connections and haustoria. The species can be distinguished from the phylogenetically related and phenotypically similar species Holtermannia corniformis and Cr. nyarrowii by some assimilatory reactions, maximum growth temperature, and sensitivity to mycocins. 相似文献
36.
Colussi F Serpa V Delabona Pda S Manzine LR Voltatodio ML Alves R Mello BL Pereira N Farinas CS Golubev AM Santos MA Polikarpov I 《Journal of microbiology and biotechnology》2011,21(8):808-817
Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatographic method for purification of ThCBHI and its initial biophysical and biochemical characterization. The ThCBHI produced by induction with microcrystalline cellulose under submerged fermentation was purified on DEAE-Sephadex A-50 media and its identity was confirmed by mass spectrometry. The ThCBHI biochemical characterization showed that the protein has a molecular mass of 66 kDa and pI of 5.23. As confirmed by smallangle X-ray scattering (SAXS), both full-length ThCBHI and its catalytic core domain (CCD) obtained by digestion with papain are monomeric in solution. Secondary structure analysis of ThCBHI by circular dichroism revealed alpha- helices and beta-strands contents in the 28% and 38% range, respectively. The intrinsic fluorescence emission maximum of 337 nm was accounted for as different degrees of exposure of ThCBHI tryptophan residues to water. Moreover, ThCBHI displayed maximum activity at pH 5.0 and temperature of 50 degrees C with specific activities against Avicel and p-nitrophenyl-β-D-cellobioside of 1.25 U/mg and 1.53 U/mg, respectively. 相似文献
37.
Ultraviolet fiber optic phototherapy of the blood and purulent cavities in patients with acute purulent lung abscesses results in a pronounced improvement of humoral immunity expressed in a tendency of all classes of immunoglobulins toward normalization. The bacterial flora of the purulent cavities and bronchial contents is markedly eradicated. 相似文献
38.
39.
B F Tolkunov I A Zhuravin A V Golubev 《Zhurnal evoliutsionno? biokhimii i fiziologii》1990,26(3):383-389
Identical facilitation of the primary response to peripheral stimuli in rats and monkeys has been induced by local superficial cooling of the cortex in the somatosensory area S1. Higher facilitation of the evoked potentials was observed in the neostriatum of rats. Correlation of the evoked potentials with primary responses was more significant with respect to temporal than amplitude parameters. In monkeys, "cool" facilitation of the cortical primary response did not result in facilitation of the evoked potential in the neostriatum. Temporal correlation between the primary response and the evoked potential was less significant, whereas correlation in the amplitude was absent. The data obtained indicate the existence of differences in functional organization of corticofugal influences upon the neostriatum in rodents and primates. 相似文献
40.
E. V. Dubrovskaya N. N. Pozdnyakova V. S. Grinev A. Yu. Muratova S. N. Golubev A. D. Bondarenkova O. V. Turkovskaya 《Russian Journal of Plant Physiology》2016,63(3):338-348
A dominant form of cationic peroxidase (PO-2) was isolated from sorghum (Sorghum bicolor L. Moench) roots and purified to electrophoretically homogeneous state. The enzyme is a monomer with mol wt of 49.7 kD. The optimum pH and the main catalytic constants (KM, Vmax, kcat) were determined for oxidation of the main substrates including Н2О2, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 2,7-diaminofluorene, syringaldazine, 2,6-dimethoxyphenol, and o-dianisidine. The KM values increased in the sequence: H2O2 < 2,7-diaminofluorene < ABTS < o-dianisidine, whereas the maximum turnover number (93.9 s–1) was found for 2,7-diaminofluorene. Based on the analysis of molecular and catalytic properties of the enzyme, it was proven that PO-2 is a typical cationic plant peroxidase. Polycyclic aromatic hydrocarbons (phenanthrene, anthracene, fluorene), 2,2'-diphenic acid, and Ni ions had no significant influence on the activity of PO-2. The enzyme was inhibited by p-aminobenzoic acid, NaN3, 1-naphthol, 9,10-anthraquinone, and 9,10-phenanthrenequinone. In the presence of NaN3, 1-naphthol, and 9,10-phenanthrenequinone, a mixed competitive/noncompetitive type of inhibition was noted. The peroxidase PO-2 was found to oxidize synthetic anthraquinone dyes, phenanthrene, and some oxygenated derivatives of polycyclic aromatic hydrocarbons (9-phenanthrol; 1-naphthol; and 1-hydroxy-2-naphthoic, salicylic, and 2,2'-diphenic acids), which indirectly confirms the coupled plant–microbial metabolism of these compounds in the root zone of sorghum. The results indicate that 9,10-phenanthrenequinone and 2,2'-diphenic acid are the products of peroxidase-catalyzed oxidation of 9-phenanthrol. 相似文献