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81.
Summary Accumulation of cobalt (60Co) by the estuarine microalgaChlorella salina has been characterized. At cobalt concentrations ranging over 3.125–100 M, a significant amount of cobalt was bound within 1 min. This was metabolism-independent and unaffected by incubation in light or dark conditions. This initial rapid phase of biosorption was followed by a slower phase of uptake which was apparently active and inhibited by incubation in the dark, or by the uncoupler dinitrophenol and the respiratory and photosynthetic inhibitor potassium cyanide in the light. For cells suspended in 10 mM Taps pH 8, cobalt biosorption followed a Freundlich adsorption isotherm. However, in the presence of 0.5 M NaCl, biosorption deviated from the Freundlich model because of competition by Na+. Cobalt biosorption was decreased by increasing concentrations of Na+, decreasing pH and the presence of Cs+, Li+, Rb+, Zn2+. Mn2+ and Sr2+ (added as chlorides). This was a result of competition between Co2+ and the other cations, including H+, for available binding sites on the cell wall and was confirmed by increased desorption of cobalt by solutions of low pH or high salinity. Increasing cell density resulted in increased removal of cobalt from solution but decreased the specific amount of cobalt taken up by the cells. 相似文献
82.
Geoffrey W. Garnham Geoffrey A. Codd Geoffrey M. Gadd 《Applied microbiology and biotechnology》1992,37(5):679-684
Summary Accumulation of [99Tc]pertechnetate ions (99TcO4
–) by the freshwater microalgae Chlorella emersonii, Chlamydomonas reinhardtii and Scenedesmus obliquus has been characterized. In all three species, accumulation consisted of a single rapid energy-independent phase (biosorption), and no energy-dependent accumulation was observed. Biosorption of 99TcO
inf4
sup–
by all three species was concentration dependent, followed a Freundlich adsorption isotherm, and was dependent on pH with increased accumulation by cells with decreasing external pH. Elevated external NaCl concentrations also caused increased accumulation of 99TcO
inf4
sup–
by the cells, as did increased external osmotic potential. Concentrations of K+, Mg2+ and Ca2+ increased accumulation of 99TcO
inf4
sup–
, but concentrations of HCO
inf3
sup–
, SO
inf4
sup2–
and CO
inf3
sup2–
decreased 99TcO
inf4
sup–
accumulation by the cells. Most of the 99TcO
inf4
sup–
accumulated by all three species was easily desorbed by 10 mm buffers at various pH values, 0.5 m NaCl, 10 mm Na2CO3 or 10 mm Na2SO4. No differences in the amount of desorption were observed between the various desorption agents used.
Correspondence to: G. M. Gadd 相似文献
83.
Accumulation of a Polyhydroxyalkanoate Containing Primarily 3-Hydroxydecanoate from Simple Carbohydrate Substrates by Pseudomonas sp. Strain NCIMB 40135 总被引:11,自引:6,他引:5 下载免费PDF全文
Geoffrey W. Haywood Alistair J. Anderson David F. Ewing Edwin A. Dawes 《Applied microbiology》1990,56(11):3354-3359
A number of Pseudomonas species have been identified which accumulate a polyhydroxyalkanoate containing mainly 3-hydroxydecanoate monomers from sodium gluconate as the sole carbon source. One of these, Pseudomonas sp. strain NCIMB 40135, was further investigated and shown to accumulate such a polyhydroxyalkanoate from a wide range of carbon sources (C2 to C6); however, when supplied with octanoic acid it produced a polyhydroxyalkanoate containing mainly 3-hydroxyoctanoate monomers. Polymer synthesis occurred in batch culture after cessation of growth due to exhaustion of nitrogen. In continuous culture under nitrogen limitation up to 16.9% (wt/wt) polyhydroxyalkanoate was synthesized from glucose as the carbon source. The monomer units are mainly of the R-(−) configuration. Nuclear magnetic resonance studies confirmed the composition of the polymer. Differential scanning calorimetry suggested that the solvent-extracted polymer contained a significant proportion of crystalline material. The weight-average molecular weight of the polymer from glucose-grown cells was 143,000. 相似文献
84.
Hereditary spherocytosis of man. Altered binding of cytoskeletal components to the erythrocyte membrane 总被引:2,自引:1,他引:1 下载免费PDF全文
John S. Hill William H. Sawyer Geoffrey J. Howlett James S. Wiley 《The Biochemical journal》1982,201(2):259-266
The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate. 相似文献
85.
86.
The amino acid sequence of a variant surface glycoprotein (VSG 117) of Trypanosoma brucei has been determined by manual sequencing of tryptic. staphylococcal protease and cyanogen bromide peptides and fragments derived from these peptides. Some overlaps needed for completion of the sequence were deduced from the nucleotide sequence of complementary DNA derived from messenger RNA coding for VSG 117. The glycoprotein consists of 470 amino acid residues with two carbohydrate chains attached at Asn420 and Asp470. No pronounced hydrophobic regions, which are characteristic of many membrane proteins, are present in the isolated glycoprotein, and the carboxy-terminal region, which is close to the membrane, is remarkably hydrophilic. These observations indicate that the molecule probably does not penetrate the lipid bilayer of the plasma membrane. The high proportion of charged residues in the carboxyterminal region is more consistent with electrostatic interaction with the polar head groups of the phospholipids. 相似文献
87.
The paper, which has an informal discussion at the end, provides a game theoretical analysis of the asymmetric “war of attrition” with incomplete information. This is a contest where animals adopt different roles like “owner” and “intruder” in a territorial conflict, and where the winner is the individual prepared to persist longer. The term incomplete information refers to mistakes in the identification of roles. The idea by Parker & Rubenstein (1981) is mathematically worked out and confirmed that there exists only a single evolutionarily stable strategy (ESS) for the model with a continuum of possible levels of persistence and no discontinuities in the increase of cost during attrition. The ESS prescribes to settle the conflict according to “who has more to gain or less to pay for persistence”. The only evolutionarily stable convention is thus to give the player access to the resource who has the role which is favoured with respect to payoffs. By contrast, it was shown earlier (Hammerstein, 1981) for various asymmetric versions of the “Hawks-Doves” model that an ESS can exist which appears paradoxical with respect to payoffs. The nature of this contrast is further analyzed by introducing elements of discreteness in the asymmetric war of attrition. It turns out that some conditions must be satisfied in order to have the possibility of an alternative ESS which is not of the above simple commonsense type. First, a decision to persist (or escalate) further in a contest must typically commit a contestant to go on fighting for a full “round”, before he can give up without danger. Second, such a “discontinuity” must occur at a level of persistence where the contest is still cheap, and, finally, errors in the identification of roles must be rare. 相似文献
88.
Geoffrey T. Evans 《Journal of theoretical biology》1980,82(2):313-315
Explanations and conditions given for the occurrence of diffusive structure in two-species ecosystem models do not generalize to systems with three or more species. 相似文献
89.
Mary M. Sherman Robert P. Borris Masaru Ogura Geoffrey A. Cordell Norman R. Farnsworth 《Phytochemistry》1980,19(7):1499-1501
A new triterpene has been isolated from the root bark of Ailanthus excelsa (Simaroubaceae) and identified as 3S,24S,25-trihydroxytirucall-7-ene. 相似文献
90.
Aspects of the predatory behaviour of free ranging Curanx melampygus have been examined in the shallow channel environment of Aldabra Atoll, Indian Ocean. Differences in the frequency of occurrence and number of fish hunting at two adjacent observation areas are described. The numbers of Caranx moving through the area appears to be influenced by tidal factors and possibly the abundance of prey and other environmental factors. The percentage offish hunting increases progressively throughout the day and is most commonly performed by solitary fish or small groups. While approximately equal numbers of Caranx move up or down stream significantly more fish engage in hunting activity when moving down stream which suggests the mechanical advantage of moving with the flow of water has advantages to the hunting fish. Several types of interspecific feeding association are described in which the Caranx is able to scavenge from others or to exploit the hunting methods of other species. More small Caranx form associations than do large Caranx . There is a relationship between the length of a hunting Caranx and the size and/or type of prey that makes an avoidance reaction to it. This relationship between different group sizes and the prey avoidance response is less clearly defined and it is suggested that whether hunting in groups or singly the final interaction is between a single predator and single prey. Ways by which group advantages may occur on grouped prey are described and are considered the result of independent individual action rather than group co-operation. Coastal observations indicate that Caranx use topographic features to shield their approach to areas of high prey density. 相似文献