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101.
Eraslan G Akdogan M Yarsan E Essiz D Sahindokuyucu F Altintas L 《Indian journal of biochemistry & biophysics》2004,41(1):57-59
Five-months-old male albino mice were subjected to an electromagnetic field (EMF) of 5 mT of magnitude with a frequency of 60 Hz for 8hr of single application. Analysis of blood sampled on hourly basis (up to 8 hr) for levels/activities of total protein, albumin, globulin, uric acid, creatinine, cholesterol, and alkaline phosphatase indicated no significant differences (p > 0.05) from that of the control group. 相似文献
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Donus Gencer Zeynep Bayramoglu Remziye Nalcacioglu Regina G. Kleespies Zihni Demirbag 《Biocontrol Science and Technology》2018,28(2):107-121
In this study, Lymantria dispar dispar larvae, collected from three different localities in Turkey, were examined for the presence of inclusion bodies under phase contrast and electron microscopes. Inclusion bodies from infected larvae were subjected to polymerase chain reaction using the conserved primers for polyhedrin (polh), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) genes. Sequence analysis confirmed that larvae collected from the three different localities contained multiple nucleopolyhedrosis viruses (MNPVs). These isolates were designated LdMNPV-T1, LdMNPV-T2 and LdMNPV-T3. Phylogenetic analyses of these isolates were performed using target genes polh, lef-8 and lef-9. Restriction endonuclease analysis of the three geographic isolates with EcoRI and PstI enzymes demonstrated some differences existed among the isolates. According to the EcoRI profile, the mean estimated size for the complete genome of each isolate (LdMNPV-T1, LdMNPV-T2 and LdMNPV-T3) was calculated to be approximately 170, 153 and 170?kb, respectively. Insecticidal activities of each isolate were tested on L. d. dispar larvae using four different viral concentrations between 103 and 106?OBs/ml. Results showed that the mortalities for LdMNPV-T1, -T2 and -T3 ranged between 13–53%, 47–100% and 46–93%, respectively. The LC50 and LC95 values of LdMNPV-T2 were not significantly different from the respective corresponding values of the other two isolates. However, isolate LdMNPV-T2 killed larvae with a LC50 value that was lower than the other two isolates. Our results suggested there are promising LdMNPV isolates in Turkey that can be used for microbial control of L. d. dispar larvae. 相似文献
103.
Achene anatomy and stomatal characteristics of eighteen Crepis L. (Asteraceae) taxa from Turkey with notes on their systematic significance
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Huseyin Inceer Nursen Aksu Kalmuk Kemal Vehbi Imamoglu Gokhan Arslan Ozge Duman Sema Hayırlıoglu‐Ayaz Gul Guner 《Nordic Journal of Botany》2018,36(8)
Achene anatomy and stomatal characteristics of eighteen Crepis taxa from Turkey are here described for the first time. In all taxa examined the pericarp is composed of several layers of sclerenchymatous and parenchymatous cells. As for the achene, differences among taxa mainly concern the pericarp structure and its thickness and width. Stomata are present on both surface of the leaf in all studied taxa and all taxa have anomocytic type stomata. However, the dimensions (length and width) and density of the stomata differ significantly among the studied taxa. In addition, the dimensions of stomata are negatively correlated with stomata density. It is concluded that achene anatomy and stomatal characteristics are useful for delimitation of Crepis taxa and a key to taxa based on these characters is provided. However, based on achene anatomy and stomatal characteristics, we found no argument for an exclusion of the Lagoseris group from Crepis as has previously been proposed. 相似文献
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Adnan Ayhanci Suzan Yaman Varol Sahinturk Ruhi Uyar Gokhan Bayramoglu Hakan Senturk Yilmaz Altuner Sila Appak Sibel Gunes 《Biological trace element research》2010,134(1):98-108
Cyclophosphamide (CP) is a widely used antineoplastic drug, which could cause toxicity of the normal cells due to its toxic
metabolites. Its urotoxicity may cause dose-limiting side effects like hemorrhagic cystitis. Overproduction of reactive oxygen
species (ROS) during inflammation is one of the reasons of the urothelial injury. Selenoproteins play crucial roles in regulating
ROS and redox status in nearly all tissues; therefore, in this study, the urotoxicity of CP and the possible protective effects
of seleno-l-methionine (SLM) on urinary bladder of rats were investigated. Intraperitoneal (i.p.) administration of 50, 100, or 150 mg/kg
CP induced cystitis, in a dose-dependent manner, as manifested by marked congestion, edema and extravasation in rat urinary
bladder, a marked desquamative damage to the urothelium, severe inflammation in the lamina propria, focal erosions, and polymorphonuclear
(PMN) leukocytes associated with occasional lymphocyte infiltration determined by macroscopic and histopathological examination.
In rat urinary bladder tissue, a significant decrease in the endogenous antioxidant compound glutathione, and elevation of
lipid peroxidation were also noted. Pretreatment with SLM (0.5 or 1 mg/kg) produced a significant decrease in the bladder
edema and caused a marked decrease in vascular congestion and hemorrhage and a profound improvement in the histological structure.
Moreover, SLM pretreatment decreased lipid peroxide significantly in urinary bladder tissue, and glutathione content was greatly
restored. These results suggest that SLM offers protective effects against CP-induced urinary bladder toxicity and could be
used as a protective agent against the drug toxicity. 相似文献
107.
Altered lymphatics in an ovine model of congenital heart disease with increased pulmonary blood flow
Datar SA Johnson EG Oishi PE Johengen M Tang E Aramburo A Barton J Kuo HC Bennett S Xoinis K Reel B Kalkan G Sajti E Osorio O Raff GW Matthay MA Fineman JR 《American journal of physiology. Lung cellular and molecular physiology》2012,302(6):L530-L540
Abnormalities of the lymphatic circulation are well recognized in patients with congenital heart defects. However, it is not known how the associated abnormal blood flow patterns, such as increased pulmonary blood flow (PBF), might affect pulmonary lymphatic function and structure. Using well-established ovine models of acute and chronic increases in PBF, we cannulated the efferent lymphatic duct of the caudal mediastinal node and collected and analyzed lymph effluent from the lungs of lambs with acutely increased PBF (n = 6), chronically increased PBF (n = 6), and age-matched normal lambs (n = 8). When normalized to PBF, we found that lymph flow was unchanged following acute increases in PBF but decreased following chronic increases in PBF. The lymph:plasma protein ratio decreased with both acute and chronic increases in PBF. Lymph bioavailable nitric oxide increased following acute increases in PBF but decreased following chronic increases in PBF. In addition, we found perturbations in the transit kinetics of contrast material through the pleural lymphatics of lambs with chronic increases in PBF. Finally, there were structural changes in the pulmonary lymphatic system in lambs with chronic increases in PBF: lymphatics from these lambs were larger and more dilated, and there were alterations in the expression of vascular endothelial growth factor-C, lymphatic vessel endothelial hyaluronan receptor-1, and Angiopoietin-2, proteins known to be important for lymphatic growth, development, and remodeling. Taken together these data suggest that chronic increases in PBF lead to both functional and structural aberrations of lung lymphatics. These findings have important therapeutic implications that warrant further study. 相似文献
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Gulay Bayramoglu Begum AltintasMeltem Yilmaz M. Yakup Arica 《Bioresource technology》2011,102(2):475-482
Chloroperoxidase (CPO) was covalently immobilized on poly(hydroxypropyl methacrylate-co-polyethyleneglycole-methacrylate) membranes, which were characterized, by swelling test, FT-IR spectroscopy, scanning electron microscopy, and contact angle measurement. The Km and Vmax values for free and immobilized CPO were found to be 34.6 and 47.2 μM, and 287.5 and 245.2 U/mg protein, respectively. The optimum pH for both the free and immobilized enzyme was observed at 3.0. The immobilized enzyme showed wide pH and temperature profiles. Most importantly, the increased thermal, storage and operational stability of immobilized CPO should depend on the creation of a comfortable strong hydrophilic microenvironment on the designed support to the host enzyme molecule. 相似文献
110.
Bayramoglu G Karagoz B Altintas B Arica MY Bicak N 《Bioprocess and biosystems engineering》2011,34(6):735-746
Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene–divinylbenzene) (P(S–DVB)) beads using surface-initiated atom
transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand
attached beads were used for reversible immobilization of lipase. The influences of pH, ionic strength, and initial lipase
concentration on the immobilization capacities of the beads have been investigated. Lipase adsorption capacity of the beads
was about 78.1 mg/g beads at pH 6.0. The K
m value for immobilized lipase was about 2.1-fold higher than that of free enzyme. The thermal, and storage stability of the
immobilized lipase also was increased compared to the native lipase. It was observed that the same support enzyme could be
repeatedly used for immobilization of lipase after regeneration without significant loss in adsorption capacity or enzyme
activity. A lipase from Mucor miehei immobilized on styrene–divinylbenzene copolymer was used to catalyze the direct esterification of butyl alcohol and butyric
acid. 相似文献