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991.
BETHAN V. PURSE BENJAMIN J. J. MCCORMICK PHILIP S. MELLOR MATTHEW BAYLIS JOHN P. T. BOORMAN DAVID BORRAS IBRAHIM BURGU RUBEN CAPELA SANTO CARACAPPA FRANCISCO COLLANTES CLAUDIO DE LIBERATO JUAN A. DELGADO ERIC DENISON GEORGI GEORGIEV MEDHI EL HARAK STEPHAN DE LA ROCQUE YOUSSEF LHOR JAVIER LUCIENTES OLGA MANGANA MIGUEL ANGEL MIRANDA NEDELCHO NEDELCHEV KYRIAKI NOMIKOU AYKUT OZKUL MICHAEL PATAKAKIS ISABEL PENA PAOLA SCARAMOZZINO ALESSANDRA TORINA DAVID J. ROGERS 《Journal of Applied Ecology》2007,44(6):1231-1242
992.
993.
Copulation and/or cohabitation with a pregnant female facilitate paternal behavior in male mice. However, their contribution to the rearing of the offspring is still not well understood. Our aims were to investigate the behavior of sires toward own or alien pups; the immediate consequences of the presence of fathers on the offspring and the behavior of the mother; and whether the exposure of juvenile males to newborn siblings, in an overlapping litters context, facilitates paternal behavior in C57BL/6 mice. We found that sires behaved paternally toward alien pups at both postpartum days 3 and 7; did not affect the behavior of the mother (e.g., licking and grooming, retrieval behavior, time in the nest, and crouching postures); and reduced the time offspring stayed alone in the nest. The exposure to newborn siblings did not promote paternal behavior in juvenile males. Therefore, sires are more paternal than usually described in the literature for laboratory mice, suggesting a facultative role in the rearing of the offspring. However, juvenile male mice, in contrast to juvenile females, could be adapted to leave the nest earlier without major contribution to the offspring. 相似文献
994.
Incubation of Strelitzia juncea seeds in an oxygen atmosphereresulted in an increase in the G6PDH activity of crude embryoextracts on day one, while radicle protrusion started on dayfive. Similarly, 6PGDH activity increased over the first 4 dof incubation in oxygen. The ratio of 6PGDH/G6PDH was 3.0<x < 3.7 regardless of treatment or incubation period. Supplying oxygen per se to dormant seed and studying its effecton the activity of the two key pentose phosphate (PP) pathwayenzymes, appear to support Roberts' hypothesis that oxygen shortagerestricts PP pathway activity in dormant seeds. Key words: Dormancy, pentose phosphate pathway, Strelitzia juncea 相似文献
995.
MIREYA DE LA GARZA BRIGIDO GALLEGOS ISAURA MEZA 《The Journal of eukaryotic microbiology》1989,36(6):556-560
Characterization of a cytochalasin D-resistant mutant of the human parasite Entamoeba histolytica capable of growing at 10 μM cytochalasin is described. The mutant cells also show resistance to 5 mM colchicine and 100 μM cytochalasin B, drugs proved deleterious for wild type trophozoites. The mutants show increased osmotic fragility and electric mobility but reduced phagocytic activity, and agglutination by Concanavalin A. On the other hand pinocytic activity remains unaltered when compared with the wild type cells. Polymerized actin, seen by staining with phalloidin, often appears polarized to one end of the trophozoites and forms few of the endocytic invaginations found in wild type amebas. An altered distribution of part of the actin could explain the differences in surface properties and motility observed in the mutant amebas. 相似文献
996.
LOUISE E. M. VET RUURD DE JONG WILANT A. VAN GIESSEN J. H. VISSER† 《Physiological Entomology》1990,15(2):243-247
ABSTRACT Odour responses of parasitic insects, in search of their hosts, can change due to experience. Leptopilina heterotoma (Thomson) (Hymenoptera; Eucoilidae), a parasitic wasp of drosophilid larvae, is known to alter its preference for odours emanating from host food substrates through learning. These kinds of behavioural modifications in insects are assumed to be the result of complex processes in the brain. The results presented in this report, however, suggest that this learning-related behavioural variation is not restricted to brain processes but that it involves changes in sensitivity of olfactory receptor neurones. 相似文献
997.
Cytospin preparations were made from 102 serous effusions for immunocytochemical staining using a panel of monoclonal antibodies including a new monoclonal antibody Ber-EP4. On cytological examination, 32 fluids were reported to contain tumour cells consistent with metastatic adenocarcinoma; 66 contained benign cells only and three were reported to contain cells suspicious of malignancy. One effusion contained tumour cells consistent with malignant mesothelioma. Positive staining of the tumour cells with Ber-EP4 was observed in the 32 effusions (100%) which contained adenocarcinoma cells. No staining of the mesothelial cells in these 32 specimens was observed. Carcinoembryonic antigen, epithelial membrane antigen Ca2 and CD15 staining of tumour cells was noted in 53%, 50%, 50% and 9% of these cases, respectively. None of the mesothelial cells in the benign effusions stained with Ber-EP4. Nor did the malignant mesothelial cells in the only case of malignant mesothelioma. These findings suggest that Ber-EP4 is a valuable addition to antibodies available for the differential diagnosis of mesothelial cells and adenocarcinoma cells in serous effusions. 相似文献
998.
999.
Fitness effects of amino acid replacements in the beta-galactosidase of Escherichia coli 总被引:7,自引:1,他引:6
Two genetic procedures were used to obtain amino acid replacements in the
lacZ-encoded beta-galactosidase in Escherichia coli. Amino acid
replacements could be obtained without regard to their effects on lactase
activity by selecting spontaneous mutations that relieved the strong
polarity of six nonsense mutations. When streaked on MacConkey- lactose
indicator plates, approximately 75% of these mutants gave strong red
lactose-fermenting colonies, and 25% gave white nonfermenting colonies.
Mutants from 11 other nonsense codons were isolated directly using
MacConkey-lactose indicator plates, on which positive color indication
requires only 0.5% of the wildtype lactase activity. Among the total of 17
codons, 25 variant beta-galactosidases were identified using
electrophoresis and thermal denaturation studies. The fitness effects of
these variant beta-galactosidases were determined using competition
experiments conducted with lactose as the sole nutrient limiting the growth
rate in chemostat cultures. Three of the replacements were deleterious, one
was selectively advantageous, and the selective effects of the remaining 21
were undetectable under conditions in which the smallest detectable
selection coefficient was approximately 0.4%/generation.
相似文献
1000.
Abstract. The effectiveness of a red-light pulse acting through phytochrome in inducing primary leaf expansion in 9-d-old etiolated bean ( Phaseolus vulgaris L. ev. Limburg) seedlings is strongly increased by a continuous far-red light (CFR) pretreatment. This increase in effectiveness of a red pulse is positively correlated with the time and the fluence rate of the CFR pretreatment. Escape from photoreversibility of this red pulse after the CFR pretreatment is extremely slow (more than 3 d). When a dark period is interposed between the end of the CFR pretreatment and the inductive red pulse the photoreversible part of the response to this pulse is highly dependent upon the photostationary state of phytochrome at the onset of the dark period.
The results give strong evidence for the synergistic activity of two components of phytochrome action during leaf growth induction, one of them acting via a very stable Pfr fraction. 相似文献
The results give strong evidence for the synergistic activity of two components of phytochrome action during leaf growth induction, one of them acting via a very stable Pfr fraction. 相似文献