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61.
Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection. 相似文献
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Maya L. Groner Luke A. Rogers Andrew W. Bateman Brendan M. Connors L. Neil Frazer Sean C. Godwin Martin Krko?ek Mark A. Lewis Stephanie J. Peacock Erin E. Rees Crawford W. Revie Ulrike E. Schl?gel 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2016,371(1689)
Effective disease management can benefit from mathematical models that identify drivers of epidemiological change and guide decision-making. This is well illustrated in the host–parasite system of sea lice and salmon, which has been modelled extensively due to the economic costs associated with sea louse infections on salmon farms and the conservation concerns associated with sea louse infections on wild salmon. Consequently, a rich modelling literature devoted to sea louse and salmon epidemiology has been developed. We provide a synthesis of the mathematical and statistical models that have been used to study the epidemiology of sea lice and salmon. These studies span both conceptual and tactical models to quantify the effects of infections on host populations and communities, describe and predict patterns of transmission and dispersal, and guide evidence-based management of wild and farmed salmon. As aquaculture production continues to increase, advances made in modelling sea louse and salmon epidemiology should inform the sustainable management of marine resources. 相似文献
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AngⅡ和PKC对心肌细胞AngⅡ 1型受体的转录调节 总被引:3,自引:0,他引:3
利用体外培养的心肌细胞,观察血管紧张素Ⅱ(AngⅡ)和蛋白激酶C(PKC)在诱导AngⅡ1型受体(AT1)基因表达及蛋白质代谢中的作用.研究结果表明:AngⅡ可诱导AT1mRNA水平一过性下调,呈时间及剂量依赖性,10nmol/LAngⅡ刺激细胞6h,引起AT1mRNA水平降低幅度最大,降至对照的51.6%±9.5%,然后逐渐回升,24h恢复至对照水平.30μmol/LH-7(PKC抑制剂)能阻断AngⅡ诱导的AT1mRNA水平的下调.0.3μmol/L的PMA(PKC激活剂)单独应用可诱导AT1mRNA水平下调达对照的43%±8%,加入AT1拮抗剂DMP811及Dup753均可阻断AngⅡ诱导的AT1mRNA水平的下调.10nmol/L的AngⅡ刺激心肌细胞96h可使蛋白含量降低至对照的73.4%±5.6%,而加药持续刺激144h可使蛋白含量较对照增加33.8%±6.3%,H-7不能阻断AngⅡ诱导的蛋白含量降低,但可有效地抑制蛋白含量的增加.以上结果提示:AngⅡ对心肌细胞AT1基因的转录和细胞的蛋白代谢有调节作用,而PKC则参与了AngⅡ的这种调节作用 相似文献
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Kimberley B. Ritter David R. Jordan Scott C. Chapman Ian D. Godwin Emma S. Mace C. Lynne McIntyre 《Molecular breeding : new strategies in plant improvement》2008,22(3):367-384
QTL for stem sugar-related and other agronomic traits were identified in a converted sweet (R9188) × grain (R9403463-2-1)
sorghum population. QTL analyses were conducted using phenotypic data for 11 traits measured in two field experiments and
a genetic map comprising 228 SSR and AFLP markers grouped into 16 linkage groups, of which 11 could be assigned to the 10
sorghum chromosomes (SBI-01 to SBI-10). QTL were identified for all traits and were generally co-located to five locations
(SBI-01, SBI-03, SBI-05, SBI-06 and SBI-10). QTL alleles from R9188 were detected for increased sucrose content and sugar
content on SBI-01, SBI-05 and SBI-06. R9188 also contributed QTL alleles for increased Brix on SBI-05 and SBI-06, and increased
sugar content on SBI-03. QTL alleles from R9403463-2-1 were found for increased sucrose content and sucrose yield on SBI-10,
and increased glucose content on SBI-07. QTL alleles for increased height, later flowering and greater total dry matter yield
were located on SBI-01 of R9403463-2-1, and SBI-06 of R9188. QTL alleles for increased grain yield from both R9403463-2-1
and R9188 were found on SBI-03. As an increase in stem sugars is an important objective in sweet sorghum breeding, the QTL
identified in this study could be further investigated for use in marker-assisted selection of sweet sorghum. 相似文献
68.
Eno E. Ekong Daniel N. Okenu Jayanti Mania-Pramanik Qing He Joseph U. Igietseme Godwin A. Ananaba Deborah Lyn Carolyn Black & Francis O. Eko 《FEMS immunology and medical microbiology》2009,55(2):280-291
The Vibrio cholerae ghost (rVCG) platform is an effective carrier and delivery system for designing efficacious Chlamydia vaccines. We investigated whether CTA2B, the nontoxic derivative of cholera toxin, can augment protective immunity conferred by an rVCG-based chlamydial vaccine and enhance cross-protection against heterologous chlamydial strains. An rVCG vaccine coexpressing chlamydial major outer membrane protein and CTA2B was genetically constructed and antigens were targeted to the inner membrane of V. cholerae before ghost production by gene E -mediated lysis. Effective immunomodulation by CTA2B was demonstrated by the ability of the vaccine construct to enhance the activation and maturation of dendritic cells in vitro . Also, C57BL/6 mice immunized via mucosal and systemic routes showed increased specific mucosal and systemic antibody and T-helper type-1 (Th1) responses, irrespective of the route. The enhanced production of IFN-γ, but not IL-4 by genital mucosal and splenic T cells, indicated a predominantly Th1 response. Clearance of the Chlamydia muridarum vaginal infection was significantly enhanced by codelivery of the vaccine with CTA2B, with the intravaginal route showing a moderate advantage. These results indicate that the rVCG-based vaccine is capable of inducing cross-protection against heterologous chlamydial serovars and that incorporation of mucosal adjuvants, such as CTA2B in the rVCG delivery platform, may enhance protective immunity. 相似文献
69.
Jennifer X. Qiao Sarah R. King Kan He Pancras C. Wong Alan R. Rendina Joseph M. Luettgen Baomin Xin Robert M. Knabb Ruth R. Wexler Patrick Y.S. Lam 《Bioorganic & medicinal chemistry letters》2009,19(2):462-468
We previously disclosed a series of highly potent FXa inhibitors bearing α-substituted (CH2NR1R2) phenylcyclopropyl P4 moieties in the pyrazolodihydropyridone core system. Herein, we describe our continuous SAR efforts in this series. Effects of the C-3 substitution of the pyrazolodihydropyridone core and the α-substitution (R group) of the cyclopropyl ring on FXa binding affinity (FXa Ki), human plasma anticoagulant activity (PT EC2×) and permeability are discussed. A set of compounds obtained from optimization of the R group and the C-3 substituent were orally bioavailable in dogs. Furthermore, representative compounds were highly efficacious in the rabbit arterio-venous shunt thrombosis model (EC50s = 29–81 nM). 相似文献