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A survey of the specific features of flow cytometry, principals of instrumentation and main parameters of the modern cell sorting cytometers is given. Analytical capacities of flow cytometry as well as the main directions of its applications in cell biology, clinical diagnostics, immunology, biotechnology and molecular biology are considered. Also a possible future development of flow cytometry instrumentation and applications in molecular biology are briefly discussed. 相似文献
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L. P. Sashchenko E. A. Dukhanina T. I. Luk’yanova O. D. Kabanova E. A. Romanova Yu. V. Shatalov D. V. Yashin N. V. Gnuchev 《Molecular Biology》2000,34(5):691-695
It has been shown that lysis of tumor target cells caused by lymphokine-activated killers is possible both upon a direct contact
and in the presence of isolated nongranular cytotoxic proteins. The contact of cytolytic lymphocytes with K-562 cells leads
to Fas L activation on the lymphocyte membrane and secretion of a broad spectrum of soluble cytotoxic proteins immunologically
related to Tag 7 described earlier. These proteins can form inactive complexes, which are reactivated upon heating and addition
of ATP. The proteins induced discrete cytolytic processes in tumor cells, differing in the rate of cytolysis and the mechanism
of the apoptotic signal transduction. Fast processes (revealed in 3 h) mediated by caspases, and slow ones (in 24 h) with
the supposed involvement of mitochondria were detected. A scheme for the lymphokine-activated killer interaction with target
tumor cells is proposed. 相似文献
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We consider examples of the recently demonstrated means of attack and defense in the host versus cancer combat at the cellular
level that involve interactions between widespread multifunctional proteins Tag7/PGRP-S, Hsp70, and Mts1/S100A4. 相似文献