排序方式: 共有68条查询结果,搜索用时 15 毫秒
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SV Tillib TI Ivanova EY Lyssuk SS Larin AV Kibardin EV Korobko PN Vikhreva NV Gnuchev GP Georgiev IV Korobko 《Biochemistry. Biokhimii?a》2012,77(6):659-665
Nanoantibodies (single-domain antibodies, nanobodies) derived from noncanonical single-chain immunoglobulins provide an attractive
tool for in vitro and in vivo diagnostics as well as for development of targeted drugs for clinical use. Nanoantibodies against several clinically important
targets have been developed and are actively investigated. However, no development of nanoantibodies against vascular endothelial
growth factor VEGF-A165 has been reported. We describe here the generation of nanoantibodies derived from single-chain Bactrian camel immunoglobulins
directed against VEGF-A165. We demonstrate that these nanoantibodies are suitable for enzyme-linked immunoassay to quantify human VEGF-A165 as well as for blocking its activity. Our results provide a basis for diagnostic kit development for quantification of VEGF-A165, which emerges as a biomarker useful in various pathological conditions. In addition, the nanoantibodies might be used for
development of therapeutic molecules targeting VEGF-A165-dependent pathological neoangiogenesis. 相似文献
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We present an efficient computational architecture designed using supervised machine learning model to predict amyloid fibril
forming protein segments, named AmylPepPred. The proposed prediction model is based on bio-physio-chemical properties of
primary sequences and auto-correlation function of their amino acid indices. AmylPepPred provides a user friendly web interface
for the researchers to easily observe the fibril forming and non-fibril forming hexmers in a given protein sequence. We expect that
this stratagem will be highly encouraging in discovering fibril forming regions in proteins thereby benefit in finding therapeutic
agents that specifically aim these sequences for the inhibition and cure of amyloid illnesses.
Availability
AmylPepPred is available freely for academic use at www.zoommicro.in/amylpeppred 相似文献24.
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E.?A.?Romanova E.?A.?Dukhanina T.?N.?Sharapova L.?P.?Sashchenko N.?V.?Gnuchev D.?V.?YashinEmail author 《Doklady biological sciences》2017,472(1):31-33
Naïve non-activated lymphocytes are capable of releasing the chemoattractant complex Tag7–Mts1 and can migrate along the gradient of its concentration. After activation of these cells by IL-2, they acquire the abilities to kill tumor cells and to release the cytotoxic Tag7–Hsp70 complex, which is accompanied by a loss of both the Tag7–Mts1-mediated lymphocyte chemotaxis and the ability to release this chemoattractant into the conditioned medium. 相似文献
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Aleksandrova ES Koralevski F Titov MI Demin AV Kozyr' AV Kolesnikov AV Tramontano A Paul S Thomas D Gabibov AG Gnuchev NV Friboulet A 《Bioorganicheskaia khimiia》2002,28(2):118-125
The catalytic monoclonal antibody 9A8 (MA 9A8), antiidiotypic to the antibody AE-2 (MA AE2) produced to the active site of acetyl cholinesterase from human erythrocytes, was subjected to a structure-function study. The specific binding of MA 9A8 to MA AE2 (K 2.26 x 10(9) M-1) was shown by the method of surface plasmon resonance, and the functional activity of MA 9A8 was demonstrated. Unlike acetyl cholinesterase, this antibody specifically reacted with the irreversible phosphonate inhibitors of esterases. A peptide map of MA 9A8 was analyzed by MALDI mass spectrometry. The Ser99 residue of its heavy chain was shown to be within the active site of the catalytic antibody. A computer modeling of the MA 9A8 active site suggested the existence of a catalytic dyad formed by Ser99 and His35. A comparison of the tertiary structures of the MA 9A8 and the 17E8 monoclonal antibody, which also exhibited an esterase activity and was produced to the stable analogue of the reaction transition state, indicated a practically complete coincidence of the structures of their presumed active sites. 相似文献
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Gabibov AG Friboulet A Thomas D Demin AV Ponomarenko NA Vorobiev II Pillet D Paon M Alexandrova ES Telegin GB Reshetnyak AV Grigorieva OV Gnuchev NV Malishkin KA Genkin DD 《Biochemistry. Biokhimii?a》2002,67(10):1168-1179
Most of the data accumulated throughout the years on investigation of catalytic antibodies indicate that their production increases on the background of autoimmune abnormalities. The different approaches to induction of catalytic response toward recombinant gp120 HIV-1 surface protein in mice with various autoimmune pathologies are described. The peptidylphosphonate conjugate containing structural part of gp120 molecule is used for reactive immunization of NZB/NZW F1, MRL, and SJL mice. The specific modification of heavy and light chains of mouse autoantibodies with Val-Ala-Glu-Glu-Glu-Val-PO(OPh)2 reactive peptide was demonstrated. Increased proteolytic activity of polyclonal antibodies in SJL mice encouraged us to investigate the production of antigen-specific catalytic antibodies on the background of induced experimental autoimmune encephalomyelitis (EAE). The immunization of autoimmune-prone mice with the engineered fusions containing the fragments of gp120 and encephalitogenic epitope of myelin basic protein (MBP89-104) was made. The proteolytic activity of polyclonal antibodies isolated from the sera of autoimmune mice immunized by the described antigen was shown. Specific immune response of SJL mice to these antigens was characterized. Polyclonal antibodies purified from sera of the immunized animals revealed proteolytic activity. The antiidiotypic approach to raise the specific proteolytic antibody as an internal image of protease is described. The second order monoclonal antibodies toward subtilisin Carlsberg revealed pronounced proteolytic activity. 相似文献