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T I Tikchonenko S E Glushakova O S Kislina N A Grodnitskaya A A Manykin B S Naroditsky 《Gene》1988,63(2):321-330
High-molecular-weight viral DNAs have been packed into proteoliposomes prepared by reverse-phase evaporation followed by phospholipid membrane targeting by influenza virus glycoprotein bound to hydrophobic 'anchors'. DNA has been encapsulated in the form of spermine condensates--toroidal structures sized approx. 0.1 micron, resistant to ultrasound. The efficiency of entrapping into liposomes reached 30% for condensed DNA of Mr up to 3 X 10(7). Specific infectivity of simian virus 40 DNA and simian adenovirus DNA packed into such proteoliposomes was 50- to 100-fold higher than that shown by free DNA preparations under Ca.phosphate-precipitation conditions. 相似文献
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S E Glushakova E V Pyzhik A D Vasiuchkov I R Erokhina R F Mar'iankova 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1992,(7-8):27-31
The conditions necessary for fusion from inside (FFWI) of the BHK-21 cell culture affected by the Lassa and Mopeya arenaviruses were studied. The fusion was shown to occur only in the slightly acid medium and at lower pH meanings for the Mopeya virus, than for the Lassa virus. 相似文献
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S E Glushakova N A Grodnitskaia B S Naroditski? O S Kislina Iu S Komarov 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1985,(7):32-36
Liposomes connected with influenza viral glycoproteins increase by 1-2 orders the specific infectiousness of DNA from SV-40 or monkey adenovirus SA7 as compared with the one registered when the standard method of calcium precipitation is used. 相似文献