全文获取类型
收费全文 | 2383篇 |
免费 | 197篇 |
国内免费 | 2篇 |
出版年
2023年 | 13篇 |
2022年 | 24篇 |
2021年 | 51篇 |
2020年 | 24篇 |
2019年 | 25篇 |
2018年 | 54篇 |
2017年 | 38篇 |
2016年 | 60篇 |
2015年 | 101篇 |
2014年 | 94篇 |
2013年 | 160篇 |
2012年 | 166篇 |
2011年 | 188篇 |
2010年 | 109篇 |
2009年 | 115篇 |
2008年 | 153篇 |
2007年 | 115篇 |
2006年 | 121篇 |
2005年 | 131篇 |
2004年 | 137篇 |
2003年 | 120篇 |
2002年 | 94篇 |
2001年 | 20篇 |
2000年 | 21篇 |
1999年 | 27篇 |
1998年 | 29篇 |
1997年 | 20篇 |
1996年 | 25篇 |
1995年 | 22篇 |
1994年 | 15篇 |
1993年 | 21篇 |
1992年 | 16篇 |
1991年 | 13篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 17篇 |
1986年 | 10篇 |
1985年 | 12篇 |
1984年 | 15篇 |
1983年 | 18篇 |
1982年 | 17篇 |
1981年 | 11篇 |
1980年 | 15篇 |
1979年 | 19篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1976年 | 17篇 |
1974年 | 9篇 |
1973年 | 10篇 |
排序方式: 共有2582条查询结果,搜索用时 125 毫秒
951.
Jose Antonio Reales‐Calderón Noemí Aguilera‐Montilla Ángel Luis Corbí Gloria Molero Concha Gil 《Proteomics》2014,14(12):1503-1518
In response to different stimuli, macrophages can differentiate into either a pro‐inflammatory subtype (M1, classically activated macrophages) or acquire an anti‐inflammatory phenotype (M2, alternatively activated macrophages). Candida albicans is the most important opportunistic fungus in nosocomial infections, and it is contended by neutrophils and macrophages during the first steps of the invasive infection. Murine macrophages responses to C. albicans have been widely studied, whereas the responses of human‐polarized macrophages remain less characterized. In this study, we have characterized the proteomic differences between human M1‐ and M2‐polarized macrophages, both in basal conditions and in response to C. albicans, by quantitative proteomics (2DE). This proteomic approach allowed us to identify metabolic routes and cytoskeletal rearrangement components that are the most relevant differences between M1 and M2 macrophages. The analysis has revealed fructose‐1,6‐bisphosphatase 1, a critical enzyme in gluconeogenesis, up‐regulated in M1, as a novel protein marker for macrophage polarization. Regarding the response to C. albicans, an M1‐to‐M2 switch in polarization was observed. This M1‐to‐M2 switch might contribute to Candida pathogenicity by decreasing the generation of specific immune responses, thus enhancing fungal survival and colonization, or instead, may be part of the host attempt to reduce the inflammation and limit the damage of the infection. 相似文献
952.
953.
954.
Yannick P. Ouedraogo Longchuan Huang Mariana P. Torrente Gloria Proni Ekaterina Chadwick Rudolf J. Wehmschulte Nasri Nesnas 《Chirality》2013,25(9):575-581
A two‐step stereoselective preparation of a goldfish pheromone, 17α,20β‐dihydroxy‐4‐pregnen‐3‐one, is reported from the readily available cortexolone in 64% overall yield. The (20S)‐epimer was also synthesized in three steps from cortexolone with an overall yield of 47%. A microscale chiroptical technique based on a host/guest complexation mechanism between the substrate and a dimeric metalloporphyrin host (tweezer) was used to confirm the stereochemical assignment, while Density Functional Theory (DFT) calculations were employed to explain the high stereoselectivity induced by the 17α‐hydroxyl and C18‐methyl groups. Chirality 00:000–000:, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
955.
956.
957.
Jos M. Paruelo Martin R. Aguiar Rodolfo A. Golluscio Rolando J. C. Len Gloria Pujol 《植被学杂志》1993,4(3):425-428
Abstract. Variation in vegetation in extra-Andean Patagonia (Argentina) was analyzed using spectral data derived from AVHRR/NOAA satellite. The study of seasonal dynamics of the Normalized Difference Vegetation Index (NDVI, i.e. a combined index of the reflection in the red and infrared bands) highlighted similarities in functional aspects between regional vegetation units which are dissimilar in a geographical, physiognomical and/or floristical way, and also suggested that gross primary production is correlated with mean annual rainfall. The first axis in a Principal Component Analysis of NDVI data was correlated (r2 = 0.90) with NDVI as integrated for the study period. The second axis was correlated (r2 = 0.50) with the differences in NDVI during the growing season, reflecting seasonality. Mean annual rainfall accounted for 60% of integrated NDVI variability among vegetation units. Much of the residual variance (62%) was accounted for by the inverse of the distance to the Atlantic Ocean, which is interpreted as an ocean effect on vegetation functioning in the extra-Andean Patagonia. 相似文献
958.
Andrés Pastor-Fernández Antonio R. Bertos Arantzazu Sierra-Ramírez Javier del Moral-Salmoral Javier Merino Ana I. de Ávila Cristina Olagüe Ricardo Villares Gloria González-Aseguinolaza María Ángeles Rodríguez Manuel Fresno Nuria Gironés Matilde Bustos Cristian Smerdou Pablo Jose Fernandez-Marcos Cayetano von Kobbe 《Aging cell》2023,22(3):e13771
The enormous societal impact of the ongoing COVID-19 pandemic has been particularly harsh for some social groups, such as the elderly. Recently, it has been suggested that senescent cells could play a central role in pathogenesis by exacerbating the pro-inflammatory immune response against SARS-CoV-2. Therefore, the selective clearance of senescent cells by senolytic drugs may be useful as a therapy to ameliorate the symptoms of COVID-19 in some cases. Using the established COVID-19 murine model K18-hACE2, we demonstrated that a combination of the senolytics dasatinib and quercetin (D/Q) significantly reduced SARS-CoV-2-related mortality, delayed its onset, and reduced the number of other clinical symptoms. The increase in senescent markers that we detected in the lungs in response to SARS-CoV-2 may be related to the post-COVID-19 sequelae described to date. These results place senescent cells as central targets for the treatment of COVID-19, and make D/Q a new and promising therapeutic tool. 相似文献
959.
Gloria García-Casado Rosa Sánchez-Monge Xose S. Puente Gabriel Salcedo 《Physiologia plantarum》1996,98(2):523-528
The only inhibitor of human salivary α-amylase identified so far in Hordeum has been isolated from barley cv. Bomi endosperm. This protein has the same N-terminal sequence (23 residues), molecular mass, and isoelectric point as one of the subunits of the barley tetrameric inhibitor previously characterized. However, enzymatic cleavage of both proteins with endoproteinase Glu-C revealed that they are products of different genes. The two isoforms have diverged in their aggregative and inhibitory properties. Thus, the subunit previously characterized forms, along with two other subunits, a tetramer active towards insect but not human salivary α-amylase, while the isoform reported here behaves as a homodimer effective against the human enzyme. These results are discussed in the context of the evolution of the cereal α-amylase inhibitor family. 相似文献
960.
Gloria García-Casado Rosa Sánchez-Monge Xose S. Puente Gabriel Salcedo 《Physiologia plantarum》1996,98(3):523-528
The only inhibitor of human salivary α-amylase identified so far in Hordeum has been isolated from barley cv. Bomi endosperm. This protein has the same N-terminal sequence (23 residues), molecular mass, and isoelectric point as one of the subunits of the barley tetrameric inhibitor previously characterized. However, enzymatic cleavage of both proteins with endoproteinase Glu-C revealed that they are products of different genes. The two isoforms have diverged in their aggregative and inhibitory properties. Thus, the subunit previously characterized forms, along with two other subunits, a tetramer active towards insect but not human salivary α-amylase, while the isoform reported here behaves as a homodimer effective against the human enzyme. These results are discussed in the context of the evolution of the cereal α-amylase inhibitor family. 相似文献