全文获取类型
收费全文 | 6117篇 |
免费 | 386篇 |
国内免费 | 2篇 |
专业分类
6505篇 |
出版年
2023年 | 22篇 |
2022年 | 39篇 |
2021年 | 79篇 |
2020年 | 41篇 |
2019年 | 50篇 |
2018年 | 118篇 |
2017年 | 75篇 |
2016年 | 123篇 |
2015年 | 210篇 |
2014年 | 238篇 |
2013年 | 314篇 |
2012年 | 490篇 |
2011年 | 555篇 |
2010年 | 246篇 |
2009年 | 224篇 |
2008年 | 429篇 |
2007年 | 348篇 |
2006年 | 402篇 |
2005年 | 361篇 |
2004年 | 372篇 |
2003年 | 315篇 |
2002年 | 288篇 |
2001年 | 158篇 |
2000年 | 175篇 |
1999年 | 107篇 |
1998年 | 47篇 |
1997年 | 28篇 |
1996年 | 40篇 |
1995年 | 39篇 |
1994年 | 30篇 |
1993年 | 43篇 |
1992年 | 32篇 |
1991年 | 30篇 |
1990年 | 32篇 |
1989年 | 29篇 |
1988年 | 21篇 |
1987年 | 23篇 |
1986年 | 19篇 |
1985年 | 27篇 |
1984年 | 19篇 |
1983年 | 29篇 |
1982年 | 30篇 |
1981年 | 24篇 |
1980年 | 26篇 |
1979年 | 27篇 |
1978年 | 20篇 |
1977年 | 18篇 |
1976年 | 19篇 |
1974年 | 10篇 |
1973年 | 8篇 |
排序方式: 共有6505条查询结果,搜索用时 15 毫秒
31.
32.
33.
Microstratification of phytoplankton in the large shallow Lake Balaton (Hungary) was studied during a 24 h period. Dissolved O2 showed biological stratification; flagellates exhibited a definite circadian rhythm. In the middle of the investigation a heavy storm broke out which was followed by the disappearance of differences between different layers of water. Storm-induced destratification is described by cluster-analysis. Abundances of dominant species changed differently in connection with the storm. Numbers of Nitzschia sp. increased due to stirring up from the sediment surface. Numbers of single-celled or colony-forming species (Cyclotella comta, Crucigenia quadrata, Coelosphaerium kuetzingianum) practically did not change. Numbers of all the three dominant filamentous species (Aphanizomenon fos-aquae f. klebahnii, Lyngbya limnetica, Planctonema lauterbornii) significantly decreased, which might be attributed to an unknown loss process and was followed by a competitive displacement by algae of small cell size. 相似文献
34.
35.
36.
37.
Assembly of the Mitochondrial Membrane System: Nuclear Suppression of a Cytochrome b Mutation in Yeast Mitochondrial DNA 总被引:1,自引:0,他引:1
In a previous study, a mitochondrial mutant expressing a specific enzymatic deficiency in co-enzyme QH2-cytochrome c reductase was described (Tzagoloff, Foury and Akai 1976). Analysis of the mitochondrially translated proteins revealed the absence in the mutant of the mitochondrial product corresponding to cytochrome b and the presence of a new low molecular weight product. The premature chain-termination mutant was used to obtain suppressor mutants with wild-type properties. One such revertant strain was analyzed genetically and biochemically. The revertant was determined to have a second mutation in a nuclear gene that is capable of partially suppressing the original mitochondrial cytochrome b mutation. Genetic data indicate that the nuclear mutation is recessive and is probably in a gene coding for a protein involved in the mitochondrial translation machinery. 相似文献
38.
Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fold purification was obtained. It was found that 5 and 10 mM MgCl2 significantly increased the bacteriolytic activity. It is a basic protein. The cell walls of Micrococcus lysodeikticus were lysed by the enzyme, and the products of digestion were purified by Amberlite CG-120 and Sephadex G-15 chromatography to facilitate the detection of amino sugars. After reduction of the oligosaccharides with sodium borohydride and acid hydrolysis, the amino sugars were identified by paper chromatography. It was found that enzyme II cleaved the glycosidic bond between N-acetylmuramic and and N-acetylglucosamine of the peptidoglycan moiety of the cell walls. Thus, the enzyme was identified as endo-beta-N-acetylmuramidase. 相似文献
39.
Alice O. Martin L. Miller J. L. Simpson C. Thomas M. S. Rzeszotarski S. Elias Gloria E. Sarto V. A. Patel 《Human genetics》1979,48(2):211-219
Summary A variant chromosome no. 21 consisting of two stalks and two satellites in tandem was detected during a survey of a human isolate. The variant segregated in three generations of a large kindred. One male had the variant no. 21, a metacentric Y, and a 47, XXY complement; however, no other evidence of chromosomal nondisjunction was found. Computer-aided analysis of sequentially stained variant no. 21 chromosomes indicated that silver-stained material corresponded to the proximal stalk region (as defined defined by Giemsa). These data support the hypothesis that human nucleolar organizers are localized to the stalks of acrocentric chromosomes. 相似文献
40.