全文获取类型
收费全文 | 142篇 |
免费 | 20篇 |
专业分类
162篇 |
出版年
2023年 | 3篇 |
2021年 | 2篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 2篇 |
2014年 | 5篇 |
2013年 | 8篇 |
2012年 | 7篇 |
2011年 | 8篇 |
2010年 | 10篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 5篇 |
2003年 | 2篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1975年 | 7篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1966年 | 4篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1954年 | 1篇 |
1953年 | 4篇 |
1952年 | 4篇 |
排序方式: 共有162条查询结果,搜索用时 15 毫秒
101.
Protected areas (PAs) have been established to conserve tropical forests, but their effectiveness at reducing deforestation is uncertain. To explore this issue, we combined high resolution data of global forest loss over the period 2000–2012 with data on PAs. For each PA we quantified forest loss within the PA, in buffer zones 1, 5, 10 and 15 km outside the PA boundary as well as a 1 km buffer within the PA boundary. We analysed 3376 tropical and subtropical moist forest PAs in 56 countries over 4 continents. We found that 73% of PAs experienced substantial deforestation pressure, with >0.1% a−1 forest loss in the outer 1 km buffer. Forest loss within PAs was greatest in Asia (0.25% a−1) compared to Africa (0.1% a−1), the Neotropics (0.1% a−1) and Australasia (Australia and Papua New Guinea; 0.03% a−1). We defined performance (P) of a PA as the ratio of forest loss in the inner 1 km buffer compared to the loss that would have occurred in the absence of the PA, calculated as the loss in the outer 1 km buffer corrected for any difference in deforestation pressure between the two buffers. To remove the potential bias due to terrain, we analysed a subset of PAs (n = 1804) where slope and elevation in inner and outer 1 km buffers were similar (within 1° and 100 m, respectively). We found 41% of PAs in this subset reduced forest loss in the inner buffer by at least 25% compared to the expected inner buffer forest loss (P<0.75). Median performance () of subset reserves was 0.87, meaning a reduction in forest loss within the PA of 13%. We found PAs were most effective in Australasia (), moderately successful in the Neotropics () and Africa (), but ineffective in Asia (). We found many countries have PAs that give little or no protection to forest loss, particularly in parts of Asia, west Africa and central America. Across the tropics, the median effectiveness of PAs at the national level improved with gross domestic product per capita. Whilst tropical and subtropical moist forest PAs do reduce forest loss, widely varying performance suggests substantial opportunities for improved protection, particularly in Asia. 相似文献
102.
The use of sequence alignments to understand protein families is ubiquitous in molecular biology. High quality alignments are difficult to build and protein alignment remains one of the largest open problems in computational biology. Misalignments can lead to inferential errors about protein structure, folding, function, phylogeny, and residue importance. Identifying alignment errors is difficult because alignments are built and validated on the same primary criteria: sequence conservation. Local covariation identifies systematic misalignments and is independent of conservation. We demonstrate an alignment curation tool, LoCo, that integrates local covariation scores with the Jalview alignment editor. Using LoCo, we illustrate how local covariation is capable of identifying alignment errors due to the reduction of positional independence in the region of misalignment. We highlight three alignments from the benchmark database, BAliBASE 3, that contain regions of high local covariation, and investigate the causes to illustrate these types of scenarios. Two alignments contain sequential and structural shifts that cause elevated local covariation. Realignment of these misaligned segments reduces local covariation; these alternative alignments are supported with structural evidence. We also show that local covariation identifies active site residues in a validated alignment of paralogous structures. Loco is available at https://sourceforge.net/projects/locoprotein/files/. 相似文献
103.
Calmodulin associated with rat liver mitochondria has been found to belong to a contaminant membranous fraction which contains different subcellular membranes. The concentration of calmodulin in this fraction is relatively high, about 1.6 micrograms/mg protein, and can not be decreased with EGTA. The calmodulin-rich membranous fraction seems to contain cytoskeletal proteins which could be responsible for the binding of calmodulin. 相似文献
104.
Patricia Hafner Ulrike Bonati Beat Erne Maurice Schmid Daniela Rubino Urs Pohlman Thomas Peters Erich Rutz Stephan Frank Cornelia Neuhaus Stefanie Deuster Monika Gloor Oliver Bieri Arne Fischmann Michael Sinnreich Nuri Gueven Dirk Fischer 《PloS one》2016,11(1)
Altered neuronal nitric oxide synthase function in Duchenne muscular dystrophy leads to impaired mitochondrial function which is thought to be one cause of muscle damage in this disease. The study tested if increased intramuscular nitric oxide concentration can improve mitochondrial energy metabolism in Duchenne muscular dystrophy using a novel therapeutic approach through the combination of L-arginine with metformin. Five ambulatory, genetically confirmed Duchenne muscular dystrophy patients aged between 7–10 years were treated with L-arginine (3 x 2.5 g/d) and metformin (2 x 250 mg/d) for 16 weeks. Treatment effects were assessed using mitochondrial protein expression analysis in muscular biopsies, indirect calorimetry, Dual-Energy X-Ray Absorptiometry, quantitative thigh muscle MRI, and clinical scores of muscle performance. There were no serious side effects and no patient dropped out. Muscle biopsy results showed pre-treatment a significantly reduced mitochondrial protein expression and increased oxidative stress in Duchenne muscular dystrophy patients compared to controls. Post-treatment a significant elevation of proteins of the mitochondrial electron transport chain was observed as well as a reduction in oxidative stress. Treatment also decreased resting energy expenditure rates and energy substrate use shifted from carbohydrates to fatty acids. These changes were associated with improved clinical scores. In conclusion pharmacological stimulation of the nitric oxide pathway leads to improved mitochondria function and clinically a slowing of disease progression in Duchenne muscular dystrophy. This study shall lead to further development of this novel therapeutic approach into a real alternative for Duchenne muscular dystrophy patients.
Trial Registration
ClinicalTrials.gov NCT02516085相似文献105.
Regional atmospheric CO2 inversion reveals seasonal and geographic differences in Amazon net biome exchange 下载免费PDF全文
Caroline B. Alden John B. Miller Luciana V. Gatti Manuel M. Gloor Kaiyu Guan Anna M. Michalak Ingrid T. van der Laan‐Luijkx Danielle Touma Arlyn Andrews Luana S. Basso Caio S. C. Correia Lucas G. Domingues Joanna Joiner Maarten C. Krol Alexei I. Lyapustin Wouter Peters Yoichi P. Shiga Kirk Thoning Ivar R. van der Velde Thijs T. van Leeuwen Vineet Yadav Noah S. Diffenbaugh 《Global Change Biology》2016,22(10):3427-3443
Understanding tropical rainforest carbon exchange and its response to heat and drought is critical for quantifying the effects of climate change on tropical ecosystems, including global climate–carbon feedbacks. Of particular importance for the global carbon budget is net biome exchange of CO2 with the atmosphere (NBE), which represents nonfire carbon fluxes into and out of biomass and soils. Subannual and sub‐Basin Amazon NBE estimates have relied heavily on process‐based biosphere models, despite lack of model agreement with plot‐scale observations. We present a new analysis of airborne measurements that reveals monthly, regional‐scale (~1–8 × 106 km2) NBE variations. We develop a regional atmospheric CO2 inversion that provides the first analysis of geographic and temporal variability in Amazon biosphere–atmosphere carbon exchange and that is minimally influenced by biosphere model‐based first guesses of seasonal and annual mean fluxes. We find little evidence for a clear seasonal cycle in Amazon NBE but do find NBE sensitivity to aberrations from long‐term mean climate. In particular, we observe increased NBE (more carbon emitted to the atmosphere) associated with heat and drought in 2010, and correlations between wet season NBE and precipitation (negative correlation) and temperature (positive correlation). In the eastern Amazon, pulses of increased NBE persisted through 2011, suggesting legacy effects of 2010 heat and drought. We also identify regional differences in postdrought NBE that appear related to long‐term water availability. We examine satellite proxies and find evidence for higher gross primary productivity (GPP) during a pulse of increased carbon uptake in 2011, and lower GPP during a period of increased NBE in the 2010 dry season drought, but links between GPP and NBE changes are not conclusive. These results provide novel evidence of NBE sensitivity to short‐term temperature and moisture extremes in the Amazon, where monthly and sub‐Basin estimates have not been previously available. 相似文献
106.
An attempt was made to compare the details of chromosome structure between two distantly related species ofDrosophila, D. melanogaster andD. hydei, which belong to different subgenera. Several short stretches of salivary gland chromosomes were selected on the basis of
presumed homologous markers, either mutants associated with chromosome breaks, or experimental puffs. Banding patterns were
compared in map diagrams, compiled from photographs. It proved possible to correlate with fair accuracy at least a short sequence
of bands in each of the examples studied. The map regions were however very different otherwise. It was not possible to judge
in how far this is a consequence of major or very small rearrangements, or other types of change.
The chromosomal location of one permanent puff and of several others some of which are formed normally during pupation, and
some of which appear after a period of oxygen deprivation, was found to be in complete agreement with genetical data which
indicate homology of chromosomes 2 (hydei) with3R (melanogaster), and of 4 with3L. On the other hand, relative position and sequence of these puffs within the chromosome are different in the two species.
Supported by the Netherlands Organization for the Advancement of Pure Research, Grant 913-28. 相似文献
107.
Gene targeting of a plasmid-borne sequence to a double-strand DNA break in Drosophila melanogaster. 总被引:4,自引:1,他引:3 下载免费PDF全文
We report an efficient and specific gene targeting method for transforming the germ line of Drosophila melanogaster. The targeting occurs during the repair of a double-strand DNA break that is induced at the white locus by the excision of a P transposable element. The break is repaired when homologous sequence is copied from a plasmid injected into the Drosophila embryo. The procedure efficiently integrates DNA into the targeted locus of the Drosophila genome. Heterologous sequence of up to 13 kbp in length can be inserted, permitting the intergration of entire genes into a common genomic site for further study. 相似文献
108.
Benthic boundary mixing and resuspension induced by internal seiches 总被引:10,自引:5,他引:5
The effect of internal seiches on horizontal hypolimnetic bottom currents and on the stationary well-mixed benthic boundary layer (BBL) induced by these currents was studied for 2 weeks in a small prealpine lake using thermistor strings, an acoustic current meter and a CTD (C: conductivity, T: temperature, D: depth) equipped with a transmissometer. 150 profiles of temperature, conductivity and transmissivity taken during two days clearly indicate the existence of a well-mixed BBL 2 to 7 m thick. This is the result of intense mixing in the zone of high shear above the sediment associated with seiching motion. The concentration of suspended or resuspended particles, mainly of organic nature, within the BBL, was 2 to 4 times greater than that measured directly above the BBL. Resuspension is thought to be associated rather with high-frequency burst-like currents with measured speeds ranging up to 7 cm s–1 than with the average bottom current speed of about 2 cm s–1. 相似文献
109.
110.
Subunit structure in the walls of sectioned microtubules was first noted by Ledbetter and Porter (6), who clearly showed that certain microtubules of plant meristematic cells have 13 wall protofilaments when seen in cross section. Earlier, protofilaments of microtubular elements had been described in negatively stained material, although exact counts of their number were difficult to obtain. In microtubular elements of axonemes, some success has been achieved in visualizing protofilaments in conventionally fixed and sectioned material (8, 10); much less success has been achieved in identifying and counting protofilaments of singlet cytoplasmic microtubules. By using glutaraldehyde-tannic acid fixation, as described by Misuhira and Futaesaku (7), Tilney et al. (12) studied microtubules from a number of sources and found that all have 13 protofilaments comprising their walls. These authors note that "...the number of subunits and their arrangement as protofilaments appear universal...". Preliminary studies of ventral nerve cord of crayfish fixed in glutaraldehyde-tannic acid indicated that axonal microtubules in this material possess only 12 protofilaments (4). On the basis of this observation, tannic acid preparations of several other neuronal and non-neuronal systems were examined. Protofilaments in microtubules from these several cell types are clearly demonstrated, and counts have been made which show that some kinds of microtubules have more or fewer protofilaments than the usual 13 and that at least one kind of microtubule has an even rather than an odd number. 相似文献