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991.
Susan Costantini Francesca Capone Marco Miele Eliana Guerriero Maria Napolitano Giovanni Colonna Giuseppe Castello 《Bioinformation》2009,4(3):92-93
Cytokines are subdivided in 12 sub-families and are described as multi-functional
molecules that play an important biological activity in host defense system
against pathogens, in homeostasis, tissue repair, cell growth and development.
CytokineDB is an annotated database that collects biological information
regarding the cytokines family in human and will be periodically updated by
including new biological information. This database is freely available online
and can be accessed at the URL: http://www.cro-m.eu/CytokineDB/ 相似文献
992.
On the hydrophobicity of nitrogen dioxide: Could there be a “lens” effect for NO2 reaction kinetics?
Solvent “lens” effects for the reaction kinetics of NO2 can be evaluated on the basis of published Henry’s law constants for nitrogen dioxide in various solvents. Water-to-organic solvent partition coefficients were derived from Henry’s law constants and used to assess the tendencies of NO2 toward fleeing the aqueous environments and concentrating in biological hydrophobic media. It is concluded, based only on the estimated aqueous medium-to-cell membrane partition coefficient for NO2, that such tendencies will be relatively small, and that they may account for an acceleration of chemical reactions in biological hydrophobic media with reaction kinetics that are first order on NO2 by a factor of approximately 3 ± 1. Thus, kinetic effects due to mass action will be relatively small but it is also important to recognize that because NO2 will tend to dissolve in cell membranes, reactions with cell membrane components will not be hindered by lack of physical solubility at these loci. In comparison to other gases, nitrogen dioxide is less hydrophobic than NO, O2 and N2. 相似文献
993.
Andrea Tinelli Daniele Vergara Roberta Martignago Giuseppe Leo Maurizio Pisanò Antonio Malvasi 《Current Genomics》2009,10(4):240-249
Among the gynaecological malignancies, ovarian cancer is one of the neoplastic forms with the poorest prognosis and with the bad overall and disease-free survival rates than other gynaecological cancers. Ovarian tumors can be classified on the basis of the cells of origin in epithelial, stromal and germ cell tumors. Epithelial ovarian tumors display great histological heterogeneity and can be further subdivided into benign, intermediate or borderline, and invasive tumors. Several studies on ovarian tumors, have focused on the identification of both diagnostic and prognostic markers for applications in clinical practice. High-throughput technologies have accelerated the process of biomolecular study and genomic discovery; unfortunately, validity of these should be still demonstrated by extensive researches on sensibility and sensitivity of ovarian cancer novel biomarkers, determining whether gene profiling and proteomics could help differentiate between patients with metastatic ovarian cancer and primary ovarian carcinomas, and their potential impact on management. Therefore, considerable interest lies in identifying molecular and protein biomarkers and indicators to guide treatment decisions and clinical follow up. In this review, the current state of knowledge about the genoproteomic and potential clinical value of gene expression profiling in ovarian cancer and ovarian borderline tumors is discussed, focusing on three main areas: distinguishing normal ovarian tissue from ovarian cancers and borderline tumors, identifying different genotypes of ovarian tissue and identifying proteins linked to cancer or tumor development. By these targets, authors focus on the use of novel molecules, developed on the proteomics and genomics researches, as potential protein biomarkers in the management of ovarian cancer or borderline tumor, overlooking on current state of the art and on future perspectives of researches.Key Words: Ovarian cancer, borderline ovarian tumors, markers, genomics, proteomics, oncogenes. 相似文献
994.
Free air CO2 enrichment (FACE) experiments in aggrading temperate forests and plantations have been initiated to test whether temperate
forest ecosystems act as sinks for anthropogenic emissions of CO2. These FACE experiments have demonstrated increases in net primary production and carbon (C) storage in forest vegetation
due to increased atmospheric CO2 concentrations. However, the fate of this extra biomass in the forest floor or mineral soil is less clear. After 6 years
of FACE treatment in a short-rotation poplar plantation, we observed an additional sink of 32 g C m−2 y−1 in the forest floor. Mineral soil C content increased equally under ambient and increased CO2 treatment during the 6-year experiment. However, during the first half of the experiment the increase in soil C was suppressed
under FACE due to a priming effect, that is, the additional labile C increased the mineralization of older SOM, whereas during
the second half of the experiment the increase in soil C was larger under FACE. An additional sink of 54 g C m−2 y−1 in the top 10 cm of the mineral soil was created under FACE during the second half of the experiment. Although, this FACE
effect was not significant due to a combination of soil spatial variability and the low number of replicates that are inherent
to the present generation of forest stand FACE experiments. Physical fractionation by wet sieving revealed an increase in
the C and nitrogen (N) content of macro-aggregates due to FACE. Further fractionation by density showed that FACE increased
C and N contents of the light iPOM and mineral associated intra-macro-aggregate fractions. Isolation of micro-aggregates from
macro-aggregates and subsequent fractionation by density revealed that FACE increased C and N contents of the light iPOM,
C content of the fine iPOM and C and N contents of the mineral associated intra-micro-aggregate fractions. From this we infer
that the amount of stabilized C and N increased under FACE treatment. We compared our data with published results of other
forest FACE experiments and infer that the type of vegetation and soil base saturation, as a proxy for bioturbation, are important
factors related to the size of the additional C sinks of the forest floor–soil system under FACE.
Author Contribution: MRH conceived of and designed the study, performed research, analyzed data, and wrote the paper; GES
conceived of and designed the study and performed research. 相似文献
995.
Molecular Determinants of the Regioselectivity of Toluene/o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1
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Eugenio Notomista Valeria Cafaro Giuseppe Bozza Alberto Di Donato 《Applied microbiology》2009,75(3):823-836
Bacterial multicomponent monooxygenases (BMMs) are a heterogeneous family of di-iron monooxygenases which share the very interesting ability to hydroxylate aliphatic and/or aromatic hydrocarbons. Each BMM possesses defined substrate specificity and regioselectivity which match the metabolic requirements of the strain from which it has been isolated. Pseudomonas sp. strain OX1, a strain able to metabolize o-, m-, and p-cresols, produces the BMM toluene/o-xylene monooxygenase (ToMO), which converts toluene to a mixture of o-, m-, and p-cresol isomers. In order to investigate the molecular determinants of ToMO regioselectivity, we prepared and characterized 15 single-mutant and 3 double-mutant forms of the ToMO active site pocket. Using the Monte Carlo approach, we prepared models of ToMO-substrate and ToMO-reaction intermediate complexes which allowed us to provide a molecular explanation for the regioselectivities of wild-type and mutant ToMO enzymes. Furthermore, using binding energy values calculated by energy analyses of the complexes and a simple mathematical model of the hydroxylation reaction, we were able to predict quantitatively the regioselectivities of the majority of the variant proteins with good accuracy. The results show not only that the fine-tuning of ToMO regioselectivity can be achieved through a careful alteration of the shape of the active site but also that the effects of the mutations on regioselectivity can be quantitatively predicted a priori. 相似文献
996.
We amplified and sequenced internal transcribed spacer (ITS) region of the nuclear ribosomal repeat from fungi in roots of
Dactylorhiza sambucina (Orchidaceae) and used the extended database to identify the mycorrhizal fungi. We molecularly identified three ITS recovered
from D. sambucina roots, one belonging to Rhizoctonia group, and two to ascomycetes, for the first time in Orchidoidae. In many cases, two sequence types were found from the same
orchid root, providing that two taxa may be involved in mycorrhizal formation (multiple mycobiont colonization). Moreover,
we demonstrated that D. sambucina plants, irrespective of their colour polymorphism, possess roots containing several fungi belonging to both asco- and basidiomycetes. 相似文献
997.
Chiara Ciaccio Grazia R. Tundo Giuseppe Grasso Daniela Marasco Magda Gioia Massimo Coletta 《Journal of molecular biology》2009,385(5):1556-1567
Insulin-degrading enzyme (IDE) is an interesting pharmacological target for Alzheimer's disease (AD), since it hydrolyzes β-amyloid, producing non-neurotoxic fragments. It has also been shown that the somatostatin level reduction is a pathological feature of AD and that it regulates the neprilysin activity toward β-amyloid.In this work, we report for the first time that IDE is able to hydrolyze somatostatin [kcat (s− 1) = 0.38 (± 0.05); Km (M) = 7.5 (± 0.9) × 10− 6] at the Phe6-Phe7 amino acid bond. On the other hand, somatostatin modulates IDE activity, enhancing the enzymatic cleavage of a novel fluorogenic β-amyloid through a decrease of the Km toward this substrate, which corresponds to the 10-25 amino acid sequence of the Aβ(1-40). Circular dichroism spectroscopy and surface plasmon resonance imaging experiments show that somatostatin binding to IDE brings about a concentration-dependent structural change of the secondary and tertiary structure(s) of the enzyme, revealing two possible binding sites. The higher affinity binding site disappears upon inactivation of IDE by ethylenediaminetetraacetic acid, which chelates the catalytic Zn2+ ion. As a whole, these features suggest that the modulatory effect is due to an allosteric mechanism: somatostatin binding to the active site of one IDE subunit (where somatostatin is cleaved) induces an enhancement of IDE proteolytic activity toward fluorogenic β-amyloid by another subunit. Therefore, this investigation on IDE-somatostatin interaction contributes to a more exhaustive knowledge about the functional and structural aspects of IDE and its pathophysiological implications in the amyloid deposition and somatostatin homeostasis in the brain. 相似文献
998.
Cell Survival and Polarity of Drosophila Follicle Cells Require the Activity of Ecdysone Receptor B1 Isoform
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Patrizia Romani Fabio Bernardi Jennifer Hackney Leonard Dobens Giuseppe Gargiulo Valeria Cavaliere 《Genetics》2009,181(1):165-175
Proper assembly and maintenance of epithelia are critical for normal development and homeostasis. Here, using the Drosophila ovary as a model, we identify a role for the B1 isoform of the ecdysone receptor (EcR-B1) in this process. We performed a reverse genetic analysis of EcR-B1 function during oogenesis and demonstrate that silencing of this receptor isoform causes loss of integrity and multilayering of the follicular epithelium. We show that multilayered follicle cells lack proper cell polarity with altered distribution of apical and basolateral cell polarity markers including atypical-protein kinase C (aPKC), Discs-large (Dlg), and Scribble (Scrib) and aberrant accumulation of adherens junctions and F-actin cytoskeleton. We find that the EcR-B1 isoform is required for proper follicle cell polarity both during early stages of oogenesis, when follicle cells undergo the mitotic cell cycle, and at midoogenesis when these cells stop dividing and undergo several endocycles. In addition, we show that the EcR-B1 isoform is required during early oogenesis for follicle cell survival and that disruption of its function causes apoptotic cell death induced by caspase. 相似文献
999.
Zhiping Wu Valentin Gogonea Xavier Lee Matthew A. Wagner Xin-Min Li Ying Huang Arundhati Undurti Roland P. May Michael Haertlein Martine Moulin Irina Gutsche Giuseppe Zaccai Joseph A. DiDonato Stanley L. Hazen 《The Journal of biological chemistry》2009,284(52):36605-36619
High density lipoprotein (HDL), the carrier of so-called “good” cholesterol, serves as the major athero-protective lipoprotein and has emerged as a key therapeutic target for cardiovascular disease. We applied small angle neutron scattering (SANS) with contrast variation and selective isotopic deuteration to the study of nascent HDL to obtain the low resolution structure in solution of the overall time-averaged conformation of apolipoprotein AI (apoA-I) versus the lipid (acyl chain) core of the particle. Remarkably, apoA-I is observed to possess an open helical shape that wraps around a central ellipsoidal lipid phase. Using the low resolution SANS shapes of the protein and lipid core as scaffolding, an all-atom computational model for the protein and lipid components of nascent HDL was developed by integrating complementary structural data from hydrogen/deuterium exchange mass spectrometry and previously published constraints from multiple biophysical techniques. Both SANS data and the new computational model, the double superhelix model, suggest an unexpected structural arrangement of protein and lipids of nascent HDL, an anti-parallel double superhelix wrapped around an ellipsoidal lipid phase. The protein and lipid organization in nascent HDL envisages a potential generalized mechanism for lipoprotein biogenesis and remodeling, biological processes critical to sterol and lipid transport, organismal energy metabolism, and innate immunity. 相似文献
1000.
Mikko Virmavirta Juha Isolehto Paavo Komi Hermann Schwameder Fabio Pigozzi Giuseppe Massazza 《Journal of biomechanics》2009,42(8):1095-1101
The take-off phase (approximately 6 m) of the jumps of all athletes participating in the individual HS-106 m hill ski jumping competition at the Torino Olympics was filmed with two high-speed cameras. The high altitude of the Pragelato ski jumping venue (1600 m) and slight tail wind in the final jumping round were expected to affect the results of this competition. The most significant correlation with the length of the jump was found in the in-run velocity (r=0.628, p<0.001, n=50). This was a surprise in Olympic level ski jumping, and suggests that good jumpers simply had smaller friction between their skis and the in-run tracks and/or the aerodynamic quality of their in-run position was better. Angular velocity of the hip joint of the best jumpers was also correlated with jumping distance (r=0.651, p<0.05, n=10). The best jumpers in this competition exhibited very different take-off techniques, but still they jumped approximately the same distance. This certainly improves the interests in ski jumping among athletes and spectators. The comparison between the take-off techniques of the best jumpers showed that even though the more marked upper body movement creates higher air resistance, it does not necessarily result in shorter jumping distance if the exposure time to high air resistance is not too long. A comparison between the first and second round jumps of the same jumpers showed that the final results in this competition were at least partly affected by the wind conditions. 相似文献