全文获取类型
收费全文 | 5677篇 |
免费 | 345篇 |
专业分类
6022篇 |
出版年
2022年 | 65篇 |
2021年 | 107篇 |
2020年 | 70篇 |
2019年 | 82篇 |
2018年 | 123篇 |
2017年 | 110篇 |
2016年 | 170篇 |
2015年 | 265篇 |
2014年 | 271篇 |
2013年 | 437篇 |
2012年 | 495篇 |
2011年 | 423篇 |
2010年 | 247篇 |
2009年 | 255篇 |
2008年 | 318篇 |
2007年 | 327篇 |
2006年 | 283篇 |
2005年 | 274篇 |
2004年 | 275篇 |
2003年 | 268篇 |
2002年 | 227篇 |
2001年 | 48篇 |
2000年 | 43篇 |
1999年 | 50篇 |
1998年 | 56篇 |
1997年 | 35篇 |
1996年 | 50篇 |
1995年 | 35篇 |
1994年 | 50篇 |
1993年 | 22篇 |
1992年 | 44篇 |
1991年 | 34篇 |
1990年 | 27篇 |
1989年 | 25篇 |
1988年 | 23篇 |
1987年 | 21篇 |
1986年 | 19篇 |
1985年 | 23篇 |
1984年 | 34篇 |
1983年 | 21篇 |
1982年 | 26篇 |
1981年 | 23篇 |
1980年 | 23篇 |
1979年 | 17篇 |
1978年 | 18篇 |
1977年 | 20篇 |
1976年 | 21篇 |
1975年 | 18篇 |
1974年 | 16篇 |
1973年 | 17篇 |
排序方式: 共有6022条查询结果,搜索用时 0 毫秒
11.
Fiorenzo A. Peverali Maurizio D'Esposito Dario Acampora Giuseppe Bunone Mario Negri Antonio Faiella Anna Stornaiuolo Maria Pannese Enrica Migliaccio Antonio Simeone Giuliano Della Valle Edoardo Boncinelli 《Differentiation; research in biological diversity》1990,45(1):61-69
Mammalian genes containing a class-I homeobox (HOX genes) are highly expressed in the embryonic nervous system. As a first step towards the molecular analysis of the role these genes play in neural cells, we studied the expression of four human HOX genes in five neuroblastoma (NB) cell lines - SK-N-BE, CHP-134, IMR-32, SK-N-SH and LAN-1 - during the process of differentiation induced by treatment with retinoic acid (RA). The four genes, HOX1D, 2F, 3E and 4B, located at corresponding positions in the four HOX loci, share a high degree of sequence similarity with the Drosophila Deformed homeotic gene and constitute a homology group, group 10. One of these genes, HOX1D, is not expressed in the cells used, whereas the other three are highly expressed in untreated and RA-induced NB cells, even though the expression pattern in the various lines is slightly different for the three genes. Our analysis reveals a complex and specific expression pattern in these lines, paving the way to an identification of different NB-cell populations by means of specific HOX gene expression schemes. On the other hand, in every line studied, morphological maturation toward a neuronal differentiated phenotype appears to be associated with increased HOX gene expression. 相似文献
12.
Valerio Vidotto Giuseppe Picerno Stefano Caramello Giovanna Paniate 《Mycopathologia》1988,104(3):129-135
The passage between the yeast and mycelial forms of Candida albicans B 311-10 was studied by using the minimal syntehtic medium of Shepherd et al. [19] modified without biotin and with low glucose concentrations. It was observed that biotin, aminoacids and particularly pH are not important factors in the dimorphism of C. albicans. The only factor of notable importance in the passage of yeast form to mycelial form in C. albicans was glucose concentration. 相似文献
13.
Giuseppe Ravizzola Gabriele Pinsi Marina Cesana Carmela De Rango Laura Peroni Prof. Adolfo Turano 《Current microbiology》1992,24(6):349-353
The protective effects of rufloxacin againstStaphylococcus aureus-induced infections were compared with those of ciprofloxacin in the granuloma pouch model in the rat. Two strains with different in vitro sensitivity to the drugs were studied. Rufloxacin concentrations persisted longer than ciprofloxacin in the exudate in the pouch cavity and were about eight times higher. Equal doses of rufloxacin and ciprofloxacin had similar antibacterial activities. However, rufloxacin inhibitedStaphylococcus aureus bacterial growth significantly longer than did ciprofloxacin. 相似文献
14.
15.
Two crystal forms of calcium carbonate were observed: calcite (utricle) and aragonite (saccule, lagena, endolymphatic sac). The first step in otolith formation is the appearance of organic structures in the macula. The subsequent step is characterized by fast growing primitive crystals with a prismatic habitus that successively transform into adult or mature crystals. With the metamorphosis, the aragonite crystals of the endolymphatic organ show clear signs of erosion that can be related to a process of CaCO3 mobilization from such deposits. 相似文献
16.
Cesare de Martino Lidia Accini Giuseppe A. cAndres Italo Archetti 《Cell and tissue research》1969,97(4):502-511
Summary Round bodies, tubular profiles and crystalline images have been studied by electron microscopy in the endothelium of seven normal young Rhesus monkeys and in the renal glomerular endothelium of two nephritic human patients. The crystalline images are most frequently formed by aggregation of round bodies, 200–240 Å in diameter. In Rhesus monkeys a variety of crystalline images are seen. On the contrary, in nephritic patients round bodies and tubular profiles are rare and less organized. In the glomerular endothelium of two normal men they were not found.The results obtained suggest that the round bodies, the tubular profiles and the crystalline images result from sectioning of a system of undulating tubules associated with the smooth endoplasmic reticulum. In nephritic patients the formation of such a tubular system could represent a change taking place within the affected cells as a pathologic response to the disease.This work was supported by U.S. Public Health Service (N.H.I.), Grant AI-04527-03-04, and by the Consiglio Nazionale delle Ricerche (C.N.R.), Contributo 115/0815/0-1365. The Authors are greatly indebted to Miss Hermina Spiele for skilful technical assistance. 相似文献
17.
Lanfranco Corazzi Giuseppe Fratto Roberto Pistolesi Giuseppe Arienti 《The Journal of membrane biology》1989,112(2):123-129
Summary Liposomes are prepared from rat brain microsomal lipid and loaded with either Tb3+ or dipicolinic acid (DPA) to test fusion with the Tb-DPA assay. They are also loaded with octadecyl Rhodamine B chloride (R18) to test fusion with the R18 assay. The addition of either Ca2+ or Mg2+ to loaded liposomes develops fluorescence with both assays. The fluorescence elicited by Mg2+ is similar to that elicited by Ca2+ if assessed with R18, but much higher if determined by Tb-DPA. The Ca2+-dependent fluorescence of the Tb-DPA complex is not suppressed by the addition of EDTA, and therefore it is internal to vesicles. The contrary is true for the Mg2+-dependent fluorescence. Rat brain microsomes can be disrupted by adding octylgucoside and reconstituted by removing it by dialysis. We use this procedure to load microsomes with DPA. This allows the use of the Tb-DPA assay for testing the fusion of rat brain microsomes. Reconstituted microsomes fuse with liposomes. This fusion has characteristics similar to those of liposome-liposome fusion. However, no microsome-microsome fusion could be detected with either method. The two methods give different results, owing to the chemical properties of the assays. Indeed Tb-DPA implies the retention of vesicle content, whereas this is not required by the R18 assay. 相似文献
18.
Inhibition of MEL cells' capacity to undergo erythroid differentiation by chemicals added during induction 总被引:1,自引:0,他引:1
Erythroid differentiation of murine erythroleukemia (MEL) cells, as induced by dimethyl sulfoxide, can be suppressed by chemicals at very low concentrations, not affecting cell viability and proliferation, if present in the culture medium between 18 and 24 h after addition of the inducer. The effect is apparent on the progeny of the treated cells and is determined, between day 3 and 5 following DMSO induction, as percent value of cells expressing the erythroid phenotype. Cultures showing decreased values are no longer terminal and a large number of clones, incapable of expressing the erythroid phenotype, can be isolated from them. In contrast, induced cultures are terminal if the added chemicals do not decrease the expression of the erythroid phenotype. Incorporation of thymidine into induced cultures reveals that maximal sensitivity of MEL cells to chemicals coincides with DNA duplication. In all affected cells, the inhibition to undergo erythroid differentiation is transmitted from one cell generation to the next. 相似文献
19.
Ninety-six CD-1 male rats were exposed to gamma-ray doses (0-25 Gy) in increments of 5 Gy. One femur, the surgically exteriorized GI tract, and the oral cavity were shielded during irradiation to protect against acute mortality from injury to the hematopoietic system, small intestine, and oral cavity. In addition, the thoraxes of half of the animals from each dose group were shielded. At approximately monthly intervals from 2 to 10 months after irradiation the hematocrit, plasma urea nitrogen (PUN), and 51Cr-EDTA clearance were measured. During the study 20 thorax-shielded and 19 thorax-irradiated animals died. All rats whose thoraxes received 25 Gy irradiation and three out of seven rats whose thoraxes received 20 Gy died 1 to 3 months postirradiation with massive pleural fluid accumulation. Shielding the thoraxes prevented this mode of death at these doses. Kidney injury was judged to be the primary cause of death of all thorax-shielded animals and 15- and 20-Gy thorax-irradiated animals. Animals with kidney damage had elevated PUN and reduced 51Cr-EDTA clearance and hematocrits. The relative merits of each of these end points in assessing radiation-induced kidney injury after total-body exposure are discussed. 相似文献
20.
Walter Malorni Pietro L. Indovina Giuseppe Arancia Stefania Meschini Maria T. Santini 《In vitro cellular & developmental biology. Plant》1990,26(4):399-410
Summary This paper describes the microscopic evidence supporting a cesium-induced delay in the fusion of chick embryo myoblast membranes
during in vitro myogenic differentiation. We have recently demonstrated that the sharp decrease in the conductivity and permittivity
of the membranes of these myogenic cells at the time of fusion is delayed 30 h by the addition of cesium to the culture medium
(Santini et al., Biochim. Biophys. Acta 945:56–64; 1988). We report here that this delay in fusion is substantiated by direct
microscopic observation and that cesium also induces ultrastructural changes in the myoblast cells themselves. Possible mechanisms
by which cesium may cause both the delay in fusion as well as the ultrastructural changes observed are discussed.
This investigation was partially supported by an Italian Consiglio Nazionale delle Ricerche grant 85.00.304.02 (to P. L. I.). 相似文献