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81.
Signal Transduction in Neuronal Death 总被引:3,自引:0,他引:3
82.
Carimi Francesco Tortorici Maria Concetta De Pasquale Fabio Crescimanno Francesco Giulio 《Plant Cell, Tissue and Organ Culture》1998,54(3):183-189
Somatic embryogenesis was induced and plant regeneration was obtained in 11 different genotypes of sweet orange navel group
[Citrus sinensis (L.) Osb.] from cultures of stigma/style explants and undeveloped ovules. Explants were cultured on 3 different
modifications of Murashige and Skoog medium: 500 mg l-1 malt extract; 500 mg l-1 malt extract and 4.6 μM kinetin; and 500
mg l-1 malt extract and 13.3 μM 6-benzylaminopurine. Sucrose (146 mM) was used as carbon source. Somatic embryogenesis occurred
1–3 months after culture initiation from undeveloped ovule and stigma/style cultures of all the genotypes tested. Somatic
embryos developed into plantlets with a high frequency (74%) after transfer to Murashige and Skoog medium supplemented with
146 mM sucrose and 500 mg l-1 malt extract. Plants were successfully transferred to soil.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
83.
Ivana Cacciatore Mara Di Giulio Erika Fornasari Antonio Di Stefano Laura Serafina Cerasa Lisa Marinelli Hasan Turkez Emanuela Di Campli Soraya Di Bartolomeo Iole Robuffo Luigina Cellini 《PloS one》2015,10(4)
Objective
The increasing prevalence of antibiotic-resistant bacterial infections led to identify alternative strategies for a novel therapeutic approach. In this study, we synthesized ten carvacrol codrugs – obtained linking the carvacrol hydroxyl group to the carboxyl moiety of sulphur-containing amino acids via an ester bond – to develop novel compounds with improved antimicrobial and antibiofilm activities and reduced toxicity respect to carvacrol alone.Method
All carvacrol codrugs were screened against a representative panel of Gram positive (S. aureus and S. epidermidis), Gram negative (E. coli and P. aeruginosa) bacterial strains and C. albicans, using broth microdilution assays.Findings
Results showed that carvacrol codrug 4 possesses the most notable enhancement in the anti-bacterial activity displaying MIC and MBC values equal to 2.5 mg/mL for all bacterial strains, except for P. aeruginosa ATCC 9027 (MIC and MBC values equal to 5 mg/mL and 10 mg/mL, respectively). All carvacrol codrugs 1-10 revealed good antifungal activity against C. albicans ATCC 10231. The cytotoxicity assay showed that the novel carvacrol codrugs did not produce human blood hemolysis at their MIC values except for codrugs 8 and 9. In particular, deepened experiments performed on carvacrol codrug 4 showed an interesting antimicrobial effect on the mature biofilm produced by E. coli ATCC 8739, respect to the carvacrol alone. The antimicrobial effects of carvacrol codrug 4 were also analyzed by TEM evidencing morphological modifications in S. aureus, E. coli, and C. albicans.Conclusion
The current study presents an insight into the use of codrug strategy for developing carvacrol derivatives with antibacterial and antibiofilm potentials, and reduced cytotoxicity. 相似文献84.
Markus K Muellner Barbara Mair Yasir Ibrahim Claudia Kerzendorfer Hannelore Lechtermann Claudia Trefzer Freya Klepsch André C Müller Ernestine Leitner Sabine Macho‐Maschler Giulio Superti‐Furga Keiryn L Bennett José Baselga Uwe Rix Stefan Kubicek Jacques Colinge Violeta Serra Sebastian MB Nijman 《Molecular systems biology》2015,11(2)
85.
Laure B. Bindels Audrey M. Neyrinck Nuria Salazar Bernard Taminiau Céline Druart Giulio G. Muccioli Emmanuelle Fran?ois Christophe Blecker Aurore Richel Georges Daube Jacques Mahillon Clara G. de los Reyes-Gavilán Patrice D. Cani Nathalie M. Delzenne 《PloS one》2015,10(6)
We tested the hypothesis that changing the gut microbiota using pectic oligosaccharides (POS) or inulin (INU) differently modulates the progression of leukemia and related metabolic disorders. Mice were transplanted with Bcr-Abl-transfected proB lymphocytes mimicking leukemia and received either POS or INU in their diet (5%) for 2 weeks. Combination of pyrosequencing, PCR-DGGE and qPCR analyses of the 16S rRNA gene revealed that POS decreased microbial diversity and richness of caecal microbiota whereas it increased Bifidobacterium spp., Roseburia spp. and Bacteroides spp. (affecting specifically B. dorei) to a higher extent than INU. INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver. POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations. Indeed, POS better than INU delayed anorexia linked to cancer progression. In addition, POS treatment increased acetate in the caecal content, changed the fatty acid profile inside adipose tissue and counteracted the induction of markers controlling β-oxidation, thereby hampering fat mass loss. Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia. 相似文献
86.
We show how a simple species distribution model can be used for the rapid estimation of potential yield and for the identification of suitable sites for farming of Tapes philippinarum in two North Adriatic lagoons (Caleri and Marinetta-Vallona, Italy) in the face of limited data. We used a two-part species distribution model with sediment type, hydrodynamism, dissolved oxygen, and salinity as predictors of T. philippinarum potential yield. The first model component uses logistic regression to identify the areas in which clams occur, while the second component uses a weighted geometric mean of suitability values to estimate the potential annual yield (kg m?2 year?1) for the sites where T. philippinarum is predicted to be present. We used site-specific yield data from Caleri and Marinetta-Vallona to estimate the weights of the geometric mean by constrained linear regression. We validated the two-part model on an independent set of yield data (R adj 2 = 0.82), and we then estimated the spatial distribution of potential yield in the two lagoons. The calibration and application of a simple species distribution model are useful tools for objectively identifying the most suitable sites for farming of T. philippinarum in North Adriatic lagoons. 相似文献
87.
Facchiano Francesco Deloye Florence Doussau Frédéric Innamorati Giulio Ashton Anthony C. Dolly J. Oliver Beninati Simone Facchiano Angelo Luini Alberto Poulain Bernard Benfenati Fabio 《Amino acids》2010,38(1):257-262
The aim of this study was to collect evidences on the role of transglutaminase (TG, E.C.2.3.2.13) in the antineoplastic properties
exerted by nimesulide (NMS), a non-steroidal anti-inflammatory drug, on murine B16-F10 melanoma cells. Treatment of melanoma
cells with nimesulide produces a considerable reduction of cell proliferation, paralleled by a remarkable decrease of the
intracellular concentration of polyamines spermidine and spermine. NMS treatment induces cancer cell differentiation, likely
through the observed enhancement of TG and tyrosinase activities and increase of melanin production, well known markers of
melanocyte differentiation. The overall results highlight the possibility that nimesulide acts as antineoplastic agent likely
through the induction of intracellular TG activity. 相似文献
88.
Jun Wang Hayley Fivecoat Lap Ho Yong Pan Emi Ling Giulio Maria Pasinetti 《Biochimica et Biophysica Acta - Proteins and Proteomics》2010,1804(8):1690-1694
Sirt1, a mammalian member of the sirtuin gene family, holds great potential for promoting longevity, preventing against disease and increasing cell survival. For example, studies suggest that the beneficial impact of caloric restriction in promoting longevity and cellular function may be mediated, in part, by Sirt1 through mechanisms involving PGC-1α, which plays important role in the regulation of cellular metabolism and inflammatory and antioxidant responses. Sirt1 may also interfere with mechanisms implicated in pathological disorders. We will present recent evidence indicating that Sirt1 may protect against Alzheimer's disease by interfering with the generation of β-amyloid peptides. We will discuss Sirt1 as a potential novel target, in addition to the development of Sirt1 activators for the prevention and treatment of Alzheimer's disease. 相似文献
89.
Giulio Lupidi Norberto Masciocchi Sartaj Tabassum Elisabetta Damiani 《Journal of inorganic biochemistry》2010,104(8):820-1608
A series of Cu(II), Zn(II) and Mn(II) coordination compounds has been synthesized by reaction of the corresponding metal salts and pyrazolyl-based ligands, i.e. the neutral 1-(2-(4-((2,2,2-tri(1H-pyrazol-1-yl)ethoxy)methyl)benzyloxy)-1,1-di(1H-pyrazol-1-yl)ethyl)-1H-pyrazole {p-C6H4[CH2OCH2C(pz)3]2, (L1), and the anionic hydridotris(3-phenyl-5-methylpyrazolyl)borate (L2)−, bis(pyrazolyl)acetate (L3) and bis(3,5-dimethylpyrazolyl)acetate (L4)−: the species [L1(CuCl2)2] (1), [L1(Cu(OAc)2)2] (2), [L1(Zn(OAc)2)2] (3), [(CuCl(L2)(HpzPh,Me)] (4), [Mn(L3)2]·2H2O, (5), [ZnCl(L3)(imH)]·MeOH [CuCl(L4)(imH)]·2H2O (7) have been obtained (HpzPh,Me = 3-phenyl-5-methylpyrazole, imH = imidazole). Complexes 1 and 4 have been structurally characterized, also using less conventional powder diffraction methods. The superoxide scavenging activity has been characterized by indirect assays including EPR analysis. All complexes exhibit superoxide scavenging activity with IC50 in the µM range and they protect against the oxidative action of peroxynitrite in different ways. 1, 4 and 7 exert both an anti- and pro-oxidant effect depending on their concentration as evaluated by EPR and fluorescence methods. The pro-oxidative effects are absent in Zn(II) and Mn(II) complexes. 相似文献
90.
Giulio Caracciolo Luciano Callipo Sofia Candeloro De Sanctis Chiara Cavaliere Daniela Pozzi Aldo Laganà 《生物化学与生物物理学报:生物膜》2010,1798(3):536-15053
Serum has often been reported as a barrier to efficient lipid-mediated transfection. Here we found that the transfection efficiency of DC-Chol-DOPE/DNA lipoplexes increases in serum. To provide insight into the mechanism of lipoplex-serum interaction, several state-of-the-art methodologies have been applied. The nanostructure of DC-Chol-DOPE/DNA lipoplexes was found to be serum-resistant as revealed by high resolution synchrotron small angle X-ray scattering, while dynamic light scattering measurements showed a marked size increase of complexes. The structural stability of DC-Chol-DOPE/DNA lipoplexes was confirmed by electrophoresis on agarose gel demonstrating that plasmid DNA remained well protected by lipids. Proteomics experiments showed that serum proteins competed for the cationic surface of lipid membranes leading to the formation of a rich a ‘protein corona’. Combining structural results with proteomics findings, we suggest that such a protein corona can promote large aggregation of intact lipoplexes. According to a recently proposed size-dependent mechanism of lipoplex entry within cells, protein corona-induced formation of large aggregates most likely results in a switch from a clathrin-dependent to caveolae-mediated entry pathway into the cells which is likely to be responsible for the observed transfection efficiency boost. As a consequence, we suggest that surface adsorption of protein corona can have a high biological impact on serum-resistant cationic formulations for in vitro and in vivo lipid-mediated gene delivery applications. 相似文献