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排序方式: 共有340条查询结果,搜索用时 296 毫秒
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Lenita de Freitas Tallarico Gisele Orlandi Introíni Amanda Bonini Flávio Dias Passos Fabrizio Marcondes Machado Eliane Pintor de Arruda 《Molluscan research.》2015,35(3):182-187
Systematics of Corbulidae supported by anatomical and conchological studies remains confused and controversial because of the considerable phenotypic plasticity of their shells. Ultrastructural spermatozoan study and molecular analyses have been performed to contribute valuable information, which could be used in taxonomy. Electron microscopy was used to analyse sperm cells from specimens of Caryocorbula (Gardner, 1926) exhibiting shell differences. The spermatozoon was of the aquasperm type, showing short acrosome, barrel-shaped nucleus, midpiece composed of four spherical mitochondria and simple flagellum. In addition, about 860?base pairs of mitochondrial large ribosomal subunit (16S rRNA) were sequenced from each individual. The consistent similarity shared by spermatozoa and DNA sequences from all studied specimens indicated that they belonged to one coherent unit, Caryocorbula caribaea (d'Orbigny, 1853), despite the extraordinary plasticity exhibited by their shells. 相似文献
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Lidiane Dal Bosco Gisele E. Weber Gustavo M. Parfitt Arthur P. Cordeiro Sangram K. Sahoo Cristiano Fantini Marta C. Klosterhoff Luis Alberto Romano Clascídia A. Furtado Adelina P. Santos José M. Monserrat Daniela M. Barros 《PloS one》2015,10(6)
Carbon nanotubes are promising nanomaterials for the diagnosis and treatment of brain disorders. However, the ability of these nanomaterials to cross cell membranes and interact with neural cells brings the need for the assessment of their potential adverse effects on the nervous system. This study aimed to investigate the biopersistence of single-walled carbon nanotubes functionalized with polyethylene glycol (SWCNT-PEG) directly infused into the rat hippocampus. Contextual fear conditioning, Y-maze and open field tasks were performed to evaluate the effects of SWCNT-PEG on memory and locomotor activity. The effects of SWCNT-PEG on oxidative stress and morphology of the hippocampus were assessed 1 and 7 days after infusion of the dispersions at 0.5, 1.0 and 2.1 mg/mL. Raman analysis of the hippocampal homogenates indicates the biopersistence of SWCNT-PEG in the hippocampus 7 days post-injection. The infusion of the dispersions had no effect on the acquisition or persistence of the contextual fear memory; likewise, the spatial recognition memory and locomotor activity were not affected by SWCNT-PEG. Histological examination revealed no remarkable morphological alterations after nanomaterial exposure. One day after the infusion, SWCNT-PEG dispersions at 0.5 and 1.0 mg/mL were able to decrease total antioxidant capacity without modifying the levels of reactive oxygen species or lipid hydroperoxides in the hippocampus. Moreover, SWCNT-PEG dispersions at all concentrations induced antioxidant defenses and reduced reactive oxygen species production in the hippocampus at 7 days post-injection. In this work, we found a time-dependent change in antioxidant defenses after the exposure to SWCNT-PEG. We hypothesized that the persistence of the nanomaterial in the tissue can induce an antioxidant response that might have provided resistance to an initial insult. Such antioxidant delayed response may constitute an adaptive response to the biopersistence of SWCNT-PEG in the hippocampus. 相似文献
265.
Lucas Tadeu Bidinotto Cristovam Scapulatempo-Neto Alan Mackay Gisele Caravina de Almeida Bernd Walter Scheithauer Gustavo Noriz Berardinelli Raul Torrieri Carlos Afonso Clara Leonir Terezinha Feltrin Marta Viana-Pereira Marileila Varella-Garcia Chris Jones Rui Manuel Reis 《PloS one》2015,10(9)
Rosette-forming glioneuronal tumor (RGNT) of the IV ventricle is a rare and recently recognized brain tumor entity. It is histologically composed by two distinct features: a glial component, resembling pilocytic astrocytoma, and a component forming neurocytic rosettes and/or perivascular rosettes. Herein, we describe a 33-year-old man with RGNT arising in the spinal cord. Following an immunohistochemistry validation, we further performed an extensive genomic analysis, using array-CGH (aCGH), whole exome and cancer-related hotspot sequencing, in order to better understand its underlying biology. We observed the loss of 1p and gain of 1q, as well as gain of the whole chromosomes 7, 9 and 16. Local amplifications in 9q34.2 and 19p13.3 (encompassing the gene SBNO2) were identified. Moreover, we observed focal gains/losses in several chromosomes. Additionally, on chromosome 7, we identified the presence of the KIAA1549:BRAF gene fusion, which was further validated by RT-PCR and FISH. Across all mutational analyses, we detected and validated the somatic mutations of the genes MLL2, CNNM3, PCDHGC4 and SCN1A. Our comprehensive molecular profiling of this RGNT suggests that MAPK pathway and methylome changes, driven by KIAA1549:BRAF fusion and MLL2 mutation, respectively, could be associated with the development of this rare tumor entity. 相似文献
266.
Patricia Tachinardi ?ivind T?ien Veronica S. Valentinuzzi C. Loren Buck Gisele A. Oda 《PloS one》2015,10(10)
Several rodent species that are diurnal in the field become nocturnal in the lab. It has been suggested that the use of running-wheels in the lab might contribute to this timing switch. This proposition is based on studies that indicate feed-back of vigorous wheel-running on the period and phase of circadian clocks that time daily activity rhythms. Tuco-tucos (Ctenomys aff. knighti) are subterranean rodents that are diurnal in the field but are robustly nocturnal in laboratory, with or without access to running wheels. We assessed their energy metabolism by continuously and simultaneously monitoring rates of oxygen consumption, body temperature, general motor and wheel running activity for several days in the presence and absence of wheels. Surprisingly, some individuals spontaneously suppressed running-wheel activity and switched to diurnality in the respirometry chamber, whereas the remaining animals continued to be nocturnal even after wheel removal. This is the first report of timing switches that occur with spontaneous wheel-running suppression and which are not replicated by removal of the wheel. 相似文献
267.
Polymer Solar Cells: Matrix Organization and Merit Factor Evaluation as a Method to Address the Challenge of Finding a Polymer Material for Roll Coated Polymer Solar Cells (Adv. Energy Mater. 10/2015)
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Eva Bundgaard Francesco Livi Ole Hagemann Jon E. Carlé Martin Helgesen Ilona M. Heckler Natalia K. Zawacka Dechan Angmo Thue T. Larsen‐Olsen Gisele A. dos Reis Benatto Bérenger Roth Morten V. Madsen Mats R. Andersson Mikkel Jørgensen Roar R. Søndergaard Frederik C. Krebs 《Liver Transplantation》2015,5(10)
268.
Making Ends Meet: Flow Synthesis as the Answer to Reproducible High‐Performance Conjugated Polymers on the Scale that Roll‐to‐Roll Processing Demands
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Martin Helgesen Jon E. Carlé Gisele A. dos Reis Benatto Roar R. Søndergaard Mikkel Jørgensen Eva Bundgaard Frederik C. Krebs 《Liver Transplantation》2015,5(9)
Continuous flow methods are employed for the controlled polymerization of the roll‐to‐roll (R2R) compatible polymer PBDTTTz‐4 including optimization and upscaling experiments. The polymerization rate and materials’ quality can be increased significantly with the continuous flow method where reaction times down to 10 min afforded PBDTTTz‐4 with high molecular weight and a constant quality. The flow method enables full control of the molecular weight via tuning of the flow speed, catalyst loading, and temperature and avoids variation in materials’ quality associated with conventional batch synthesis. Upscaling from 300 mg batch synthesis to 10 g flow synthesis affords PBDTTTz‐4 with a production rate of up to 120 g day?1 for a very simple in‐house build flow reactor. An average power conversion efficiency (PCE) of 3.5% is achieved on a small scale (1 cm2) and an average PCE of 3.3% is achieved on a large scale (29 cm2). This shows that small device efficiencies can be scaled when using full R2R processing of flexible and encapsulated carbon‐based modules without the use of vacuum, indium‐tin‐oxide, or silver, with the best achieving a PCE of 3.8% PCE. 相似文献
269.
Emma J. Gagen Alan Levett Anat Paz Heike Bostelmann Rafael Borges da Silva Valadares José Augusto Pires Bitencourt Markus Gastauer Gisele Lopes Nunes Guilherme Oliveira Paulo M. Vasconcelos Gene W. Tyson Gordon Southam 《Microbial biotechnology》2020,13(6):1960-1971
Accelerating microbial iron cycling is an innovative environmentally responsible strategy for mine remediation. In the present study, we extend the application of microbial iron cycling in environmental remediation, to include biocementation for the aggregation and stabilization of mine wastes. Microbial iron reduction was promoted monthly for 10 months in crushed canga (a by-product from iron ore mining, dominated by crystalline iron oxides) in 1 m3 containers. Ferrous iron concentrations reached 445 ppm in treatments and diverse lineages of the candidate phyla radiation dominated pore waters, implicating them in fermentation and/or metal cycling in this system. After a 6-month evaporation period, iron-rich cements had formed between grains and 20-cm aggregates were recoverable from treatments throughout the 1-m depth profile, while material from untreated and water-only controls remained unconsolidated. Canga-adapted plants seeded into one of the treatments germinated and grew well. Therefore, application of this geobiotechnology offers promise for stabilization of mine wastes, as well as re-formation of surface crusts that underpin unique and threatened plant ecosystems in iron ore regions. 相似文献
270.