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991.
Gutierrez María Florencia Epele Luis Beltrán Mayora Gisela Aquino Diego Mora Celeste Quintana Rubén Mesa Leticia 《Hydrobiologia》2022,849(16):3463-3480
Hydrobiologia - The Paraná River Delta is one of the most biodiverse and largest mosaic of wetland ecosystems in South America. Yet its natural hydrological patterns are being strongly... 相似文献
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Jiawen Yong Julia von Bremen Sabine Groeger Gisela Ruiz-Heiland Sabine Ruf 《Journal of cellular and molecular medicine》2021,25(20):9710-9723
Hypoxia-induced apoptosis of cementoblasts (OCCM-30) may be harmful to orthodontic treatment. Hypoxia-inducible factor 1-alpha (HIF-1α) mediates the biological effects during hypoxia. Little is known about the survival mechanism capable to counteract cementoblast apoptosis. We aimed to investigate the potential roles of HIF-1α, as well as the protein-protein interactions with ERK1/2, using an in-vitro model of chemical-mimicked hypoxia and adipokines. Here, OCCM-30 were co-stimulated with resistin, visfatin or ghrelin under CoCl2-mimicked hypoxia. In-vitro investigations revealed that CoCl2-induced hypoxia triggered activation of caspases, resulting in apoptosis dysfunction in cementoblasts. Resistin, visfatin and ghrelin promoted the phosphorylated ERK1/2 expression in OCCM-30 cells. Furthermore, these adipokines inhibited hypoxia-induced apoptosis at different degrees. These effects were reversed by pre-treatment with ERK inhibitor (FR180204). In cells treated with FR180204, HIF-1α expression was inhibited despite the presence of three adipokines. Using dominant-negative mutants of HIF-1α, we found that siHIF-1α negatively regulated the caspase-8, caspase-9 and caspase-3 gene expression. We concluded that HIF-1α acts as a bridge factor in lengthy hypoxia-induced apoptosis in an ERK1/2-dependent pathway. Gene expressions of the caspases-3, caspase-8 and caspase-9 were shown to be differentially regulated by adipokines (resistin, visfatin and ghrelin). Our study, therefore, provides evidence for the role of ERK1/2 and HIF-1α in the apoptotic response of OCCM-30 cells exposed to CoCl2-mimicked hypoxia, providing potential new possibilities for molecular intervention in obese patients undergoing orthodontic treatment. 相似文献
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Gisela C Stotz Cristian Salgado-Luarte Alonso T Vigil Henry J De La Cruz Víctor Pastn-Marambio Ernesto Gianoli 《Annals of botany》2021,127(5):669
Background and AimsAridity is increasing in many regions of the world, but microclimatic conditions may buffer plant communities from the direct effects of decreased precipitation, creating habitat islands. However, reduced precipitation can also impact these communities indirectly by decreasing the suitability of the surrounding habitat, thus limiting incoming propagules and increasing the chances of population decline and species loss. We test whether decreased precipitation results in loss of species and functional diversity within habitat islands, evaluating in particular whether declines in species diversity and abundance are less likely to result in loss of functional diversity if species/individual loss is stochastic (i.e. independent of species/individual traits) and communities/populations are functionally redundant.MethodsLomas communities are discrete plant communities embedded in the Atacama Desert, maintained by the microclimatic conditions created by fog. We recorded species and functional diversity in six Lomas communities along a 500 km long precipitation gradient in northern Chile. Functional traits were measured in 20 individuals per species, in those species that accounted for approx. 75 % of the abundance at each site. We calculated functional diversity and functional redundancy of the community, and intraspecific functional variation.Key ResultsDecreased precipitation was associated with lower species diversity and lower species abundances. However, no traits or functional strategies increased or decreased consistently with precipitation, suggesting stochastic species/individual loss. Species with stress-tolerant strategies were predominant in all sites. Although species diversity decreased with decreasing precipitation, functional diversity remained unchanged. Lower functional redundancy in the drier sites suggests that mainly functionally redundant species were lost. Likewise, intraspecific functional variation was similar among communities, despite the lower species abundance in drier sites.ConclusionsDecreased precipitation can impact habitat island communities indirectly by decreasing the suitability of the surrounding habitat. Our results support the idea that a stochastic loss of species/individuals from functionally redundant communities and populations does not result in loss of functional diversity. 相似文献
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Laure Cuny Daniel Pfaff Jonas Luther Florian Ranzinger Peter Ödman Johannes Gescher Gisela Guthausen Harald Horn Andrea Hille-Reichel 《Biotechnology and bioengineering》2019,116(10):2687-2697
In white biotechnology research, the putative superiority of productive biofilms to conventional biotransformation processes based on planktonic cultures has been increasingly discussed in recent years. In the present study, we chose lactic acid production as a model application to evaluate biofilm potential. A pure culture of Lactobacillus bacteria was grown in a tubular biofilm reactor. The biofilm system was cultivated monoseptically in a continuous mode for more than 3 weeks. The higher cell densities that could be obtained in the continuous biofilm system compared with the planktonic culture led to a significantly increased space-time yield. The productivity reached 80% of the maximum value 10 days after start-up and no subsequent decline was observed, confirming the suitability of the system for long-term fermentation. The analysis of biofilm performance revealed that productivity increases with the flow velocity. This is explained by the reduced retention time of the liquid phase in the reactor, and, thus, a minor pH drop caused by the released lactic acid. At low flow velocities, the pH drops to a value where growth and production are significantly inhibited. The biofilm was visualized by magnetic resonance imaging to analyze biofilm thickness. To deepen the understanding of the biofilm system, we used a simple model for cell growth and lactic acid production. 相似文献
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The disc-gel electrophoretograms of aqueous crude extracts of organisms containing phycochromoproteins (Cyanobacteria, Rhodo- and Cryptophyta) give colored ‘fingerprints’, which are species specific and in many cases strain specific also (infraspecific variability). It was found that in a slightly acidic system (pH 4,3) the electrophoretograms in one single gel concentration (16% PAA) or the Hedrick-Smith plots for the phycochromoprotein bands represent a chemotaxonomic key for identification of Cyanobacteria species. The results summarized here in a list of chemotaxonomical and numerical data for 23 strains, allow one to distinguish between the size and charge isomers of the investigated biliproteids. 相似文献
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Gisela P. Concepcion 《Bioscience Hypotheses》2009,2(1):24-26
Prions are multi-stable proteins that can assume at least two stable conformations: a normal one that is detergent-soluble and is protease-sensitive, and a pathogenic one that is protease-resistant and that forms detergent-insoluble fibrillar aggregates. The fibrillar aggregates have been implicated in various neurodegenerative disorders. The normal biological function of prions is not known, yet the high conservation of the sequences of prions among distantly related animals strongly suggests a common and important function. There is now increasing evidence that prions, which are abundant in nervous tissue, may in fact be involved in memory retention. We propose that electrical activity at the synapse induces the surrounding prion molecules to aggregate. The aggregates serve to hold together the synaptic connection between neurons which are interacting at the instant the sensory stimulus is received. The set of neurons connected in this manner then form the neuronal circuit which is associated with the particular stimulus. We propose that the stronger the electrical activity, the greater will be the aggregations. Long-lasting memory will result from traumatic, or exciting, experiences. Further, the aggregations will be maintained, or reinforced, by repeated stimulation of the same set of neurons. Memory loss will occur when those aggregates dissolve. 相似文献
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